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  • Springer  (200)
  • PANGAEA  (41)
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  • 1
    Digitale Medien
    Digitale Medien
    Effect of pH on the calcium metabolism of isolated rat kidney cells (1979)
    Springer
    The journal of membrane biology 48 (1979), S. 325-341 
    Details
    ISSN: 1432-1424
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary The effects of metabolic and respiratory acidosis and alkalosis on cellular calcium metabolism were studied in rat kidney cells dispersed with collagenase. In both types of acidosis, the intracellular pH, total cell calcium, and the cell relative radioactivity after 60 min of labeling are significantly depressed. Kinetic analysis of45Ca desaturation curves shows that acidosis decreases all three cellular calcium pools and depresses calcium fluxes between the superficial and cytosolic pools and between the cytosolic and mitochondrial pools. In alkalosis the intracelluar pH, the total cell calcium, and the cell relative radioactivity are significantly increased. Kinetic studies show that in alkalosis, only the mitochondrial pool is consistently increased. Calcium exchange between the mitochondrial and cytosolic pool is increased in metabolic alkalosis only. These results suggest that hydrogen ion is an important modulator of calcium metabolism, and that the intracellular pH rather than extracellular pH is the critical factor in determining the calcium status of cells during altered acid-base conditions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01869444
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Effects of calcium ionophores on the transport and distribution of calcium in isolated cells and in liver and kidney slices (1978)
    Springer
    The journal of membrane biology 38 (1978), S. 51-72 
    Details
    ISSN: 1432-1424
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Summary The effects of calcium ionophores on cellular calcium metabolism were studied in cultured kidney cells, in cells freshly isolated from rat kidney, and in liver and kidney slices. In isolated cells, these ionophores decreased the total cellular Ca content and the mitochondrial Ca.45Ca efflux from prelabelled cells was also stimulated even in the absence of extracellular Ca. In slices, the ionophore A23187 increased the total slice Ca and the uptake of45Ca. However, the mitochondria isolated from these slices treated with the ionophore had a lower total Ca and a depressed relative radioactivity. These results suggest that the increased cytosolic Ca produced by Ca ionophores may be due to mobilization of intracellular Ca stores rather than to a net shift of Ca from the extracellular fluids to the cell.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01875162
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Immunocytochemical localization of patatin, the major glycoprotein in potato (Solanum tuberosum L.) tubers (1989)
    Springer
    Planta 178 (1989), S. 176-183 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Glycoprotein ; Lipid acyl hydrolyse ; Patatin (immunocytochemical localization) ; Solanum (patatin localization) ; Vacuole
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Patatin is a family of glycoproteins with an apparent molecular weight of 40 kDa. The protein is synthesized as a pre-protein with a hydrophobic signal sequence of 23 amino acids. Using different immunocytochemical methods we determined the tissue-specific as well as subcellular localization of the patatin protein. Since antibodies raised against patatin showed crossreactivity with glycans of other glycoproteins, antibodies specific for the protein portion of the glycoprotein were purified. Using these antibodies for electron-microscopical immunocytochemistry, the protein was found to be localized mainly in the vacuoles of both tubers and leaves of potatoes (Solanum tuberosum L.) induced for patatin expression. Neither cell walls nor the intercellular space contained detectable levels of patatin protein. Concerning the tissue specificity, patatin was mainly found in parenchyma cells of potato tubers. The same distribution was observed for the esterase activity in potato tubers.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00393192
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    High-pressure freezing of soybean nodules leads to an improved preservation of ultrastructure (1992)
    Springer
    Planta 188 (1992), S. 155-163 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Bradyrhizobium ; Electron microscopy ; Glycine (root nodules) ; High-pressure freezing ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract High-pressure freezing of chemically untreated nodules of soybean (Glycine max (L.) Merr.), in sharp contrast to chemical fixation and prefixation, appears to preserve the ultrastructure close to the native state. This is supported by the observation that the peribacteroid membrane of high-pressure-frozen samples is tightly wrapped around the bacteroids, a finding that is fully consistent with the current views on the physiology of oxygen and metabolite transport between plant cytosol and bacteroids. In soybean root nodules, the plant tissue and the enclosed bacteria are so dissimilar that conventional aldehyde-fixation procedures are unable to preserve the overall native ultrastructure. This was demonstrated by high-pressure freezing of nodules that had been pre-fixed in glutaraldehyde at various buffer molalities: no buffer strength tested preserved all ultrastructural aspects that could be seen after high-pressure freezing of chemically untreated nodules.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216809
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Zur Molekulargewichtsverteilung von Blutersatzpräparaten aus Polyvinylpyrolidon (1951)
    Springer
    Cellular and molecular life sciences 7 (1951), S. 96-97 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The molecular weight distribution of two commercial blood substitutes of the polyvinyl-pyrrolidone type is determined. The width of this distribution is discussed in relation to excretion experiments. It is shown by histological methods that fractions of polyvinylalcohol of an average molecular weight of 75,000 are retained in the organs to a greater extent than fractions of an average molecular weight of 25,000.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02165556
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Beeinflussung tierexperimenteller Leukopenien durch Vitamin A (1948)
    Springer
    Cellular and molecular life sciences 4 (1948), S. 445-445 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary In high doses synthetic vitamin A proved to have a leucopoetic effect on the experimental leucopenia of rats. In the treatment of the succinylsulfathiazol leucopenia vitamin A does not equal the effect of folic acid, in the treatment of the milk leucopenia however it is superior to the latter.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02145005
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Zur Frage der Hemmung des Thiouracilkropfes der Ratte (1949)
    Springer
    Cellular and molecular life sciences 5 (1949), S. 362-364 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The inhibition of thiouracil goitre in the rat by the methyl ether of vitamin A reported in previous investigations is not to be referred to vitamin A, but to the iodine that is bound to vitamin A in the course of its synthesis. The relation between thiouracil and iodine compounds with different mobility of the iodine: NaI (II), the iodine-containing methyl ether of vitamine A (III), 2:4-dioxo-3:3-diethyl-5-iodine-tetrahydropyridin (IV),N-(4-oxy-3:5-diiodine-benzoyl)-diiodine-DL-tyrosine (V), and 1:4-dioxy-2:3-diiodine-buten-(2) were studied in the thyroid gland of the rat by histological investigation of the degree of development of the thiouracil goitre and by determination of the iodine content of the thyroid. The iodine deprivation caused by thiouracil is definitely inhibited by the iodine compounds named, regardless of the motility of the iodine. The histologically demonstrable thiouracil goitre is likewise inhibited by these compounds, and here the degree of inhibition increases with the motility of the iodine.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02174517
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    Hemmung des Thiouracilkropfes der Ratte durch synthetischen Vitamin-A-Methyläther (1948)
    Springer
    Cellular and molecular life sciences 4 (1948), S. 232-233 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The hyperplasia and hyperaemia of the thyreoidea of rats caused by thiouracil treatment can be largely avoided by simultaneous doses of vitamin-A methylether.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02155378
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    Changes in magnetic properties induced by heavy-ion irradiation in magnetic insulators as seen by Mössbauer spectroscopy (1996)
    Springer
    Il nuovo cimento della Società Italiana di Fisica 18 (1996), S. 337-340 
    Details
    ISSN: 0392-6737
    Schlagwort(e): Atoms and molecules ; Magnetic ordered materials: other intrinsic properties ; Magnetomechanical and magnetoelectric effects, magnetostriction ; Mössbauer effect: other γ-ray spectroscopy ; Conference proceedings
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Summary The high-energy heavy-ion irradiation of magnetic materials induces not only amorphous matter in the ion tracks but also improvement or even creation of new magnetic properties in a stressed shell surrounding the track core. Two examples are presented in this paper: magnetization creation in the spinel ZnFe2O4 after Kr, Xe and Pb irradiations and trapping of magnetic domains in another spinel, the magnetite Fe3O4, after lead irradiation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02458914
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Simple GA C T A repeats characterize the X chromosomal heterochromatin of Microtus agrestis, European field vole (Rodentia, Cricetidae) (1988)
    Springer
    Chromosoma 96 (1988), S. 213-219 
    Details
    ISSN: 1432-0886
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The sex chromosomes of Microtus agrestis are extremely large due to the accumulation of constitutive heterochromatin. We have identified two prominent satellite bands of 2.0 and 2.8 kb in length after HaeIII and HinfI restriction enzyme digestion of genomic DNA, respectively. These satellites are located on the heterochromatic long arm of the X chromosome as shown using Microtus x mouse somatic cell hybrids. By in-gel hybridization with oligonucleotide probes, the organization of the two satellites was studied: among the many copies of the simple tandem tetranucleotide repeat GATA are interspersed rare single GACA tetramers. One of the satellites also harbours related GGAT simple tandem repeats. In situ hybridizations with plasmid-carried or oligonucleotide GA C T A probes show clustered silver grains on the long and short arm of the X chromosome. Interspersion of differently organized (GATA)n elements is also demonstrable in the autosomal complement and on the Y chromosome. These results are discussed in the context of the evolution of vertebrate sex chromosomes in relation to heterochromatin and simple repetitive DNA sequences.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00302360
    Standort Signatur Erwartet Verfügbarkeit
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