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  • 1965-1969  (16)
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  • 1
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 9 (2002), S. 3402-3412 
    ISSN: 1089-7674
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: Measurements of the electron temperature, Te, and density, ne, during the energy quench of a major disruption showed that the onset of Te erosion in the neighborhood of the m/n=2/1 O point at the low field side (LFS) accelerates the well-known m/n=1/1 erosion of the core temperature. During this phase Te(r) is only partially flat in the region between the q=2 and the q=1 surfaces and ne(r) decreases in the core and increases inside the m/n=2/1 island. Immediately after the flattening of Te(r) a large peak in Te and to a lesser extent in ne has been observed. This peak is radially localized at the q=2 radius at the LFS, is very short lived and is poloidally asymmetric. Te profiles measured by the heterodyne radiometer and the Thomson scattering agree very well up to the time Te(r) flattens but afterwards can be a factor of two different. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 81 (2002), S. 592-594 
    ISSN: 1077-3118
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: By laser-interference lithography, reactive-ion etching, and selective wet-chemical etching using a citric acid-based solution, we have fabricated large periodic arrays of AlGaAs microdisks with periods of 4 μm and disk diameters between 1.5 and 2 μm. The arrays are characterized by temperature-dependent photoluminescence spectroscopy. Taking into account the below-threshold absorption of the quantum wells inside the disks, we get disk quality factors close to the theoretical maximum value. We demonstrate that our technique allows one also to produce one-dimensionally or two-dimensionally coupled arrays of microdisks. © 2002 American Institute of Physics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: A mutational analysis of the essential nuclear actin-related protein of Saccharomyces cerevisiae, Act3p/Arp4p, was performed. The five residues chosen for substitution were amino acids conserved between actin and Act3p/Arp4p, the tertiary structure of which most probably resembles that of actin. Two thermosensitive (ts) mutants, a single and a double point mutant, and one lethal double point mutant were obtained. Both ts mutants were formamide-sensitive which supports a structural relatedness of Act3p/Arp4p to actin; they were also hypersensitive against hydroxyurea and ultraviolet irradiation pointing to a possible role of Act3p/Arp4p in DNA replication and repair. Their ‘suppressor of Ty’ (SPT) phenotype, ob-served with another ts mutant of Act3p/Arp4p before, suggested involvement of Act3p/Arp4p in transcription regulation. Accordingly, genome-wide expression profiling revealed misregulated transcription in a ts mutant of a number of genes, among which increased expression of various stress-responsive genes (many of them requiring Msn2p/Msn4p for induction) was the most salient result. This provides an explanation for the mutant's enhanced resistance to severe thermal and oxidative stress. Thus, Act3p/Arp4p takes an important part in the repression of stress-induced genes under non-stress conditions.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    ISSN: 1520-5126
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    s.l. : American Chemical Society
    Journal of the American Chemical Society 90 (1968), S. 6071-6077 
    ISSN: 1520-5126
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    s.l. : American Chemical Society
    Journal of the American Chemical Society 90 (1968), S. 6077-6082 
    ISSN: 1520-5126
    Quelle: ACS Legacy Archives
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Upregulation of the ATP-binding cassette (ABC) transporter genes CDR1 and CDR2 (Candidadrug resistance 1 and 2) is a common mechanism observed in Candida albicans clinical isolates developing resistance to the class of azole antifungals. In this work, the regulatory elements of both genes were delimited using a reporter system in an azole-susceptible strain exposed to oestradiol, which allows transient induction of these genes. We found two regulatory elements in the CDR1 promoter: one responsible for basal expression (basal expression element; BEE) and the other required for oestradiol responsiveness (drug-responsive element I; DREI). In the CDR2 promoter, a single regulatory element responsible for oestradiol responsiveness (DREII) was detected. Both DREs shared a consensus of 21 bp with the sequence 5′-CGGA(A/T)ATCGGATATTTTTTTT-3′ having no equivalent to known eukaryotic regulatory sequence. Consistent with this finding, two other C. albicans genes identified by a search for the presence of DRE in the C. albicans genome sequence database were responsive to oestradiol. Finally, the regulatory elements found in CDR1 and CDR2 were also functional in an azole-resistant strain with constitutive high expression of both transporters. These results suggest that, although CDR1 and CDR2 upregulation can be obtained by transient drug-induced and constitutive upregulation, these two processes converge to the same regulatory elements and probably mobilize the same trans-acting factors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Structural genes of phospholipid biosynthesis in the yeast Saccharomyces cerevisiae are transcriptionally co-regulated by ICRE (inositol/choline-responsive element) promoter motifs. Gene activation by an ICRE is mediated by binding of the Ino2/Ino4 transcription factor, whereas repression in the presence of high concentrations of inositol and choline (IC) requires an intact Opi1 repressor. However, the mechanism of specific repression and the functional interplay among these regulators remained unclear from previous work. Using in vivo as well as in vitro interaction assays, we show binding of the pleiotropic repressor Sin3 to the pathway-specific regulator Opi1. The paired amphipathic helix 1 (PAH1) within Sin3 and OSID (Opi1–Sin3 interaction domain) in the N-terminus of Opi1 were mapped as contact sites. The regulatory significance of the Opi1–Sin3 interaction was shown by the obvious deregulation of an ICRE-dependent reporter gene in a sin3 mutant. Opi1 also interacts with a newly identified functional domain of the transcriptional activator Ino2 (RID, repressor interaction domain). These results define the molecular composition of the transcription complex mediating control of ICRE-dependent genes and allow a hypothesis on the flow of regulatory information in response to phospholipid precursors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1365-2958
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: In the yeast Saccharomyces cerevisiae, genes involved in phospholipid biosynthesis are activated by ICRE (inositol/choline-responsive element) up-stream motifs and the corresponding heterodimeric binding factor, Ino2 + Ino4. Both Ino2 and Ino4 contain basic helix–loop–helix (bHLH) domains required for ICRE binding, whereas transcriptional activation is mediated exclusively by Ino2. In this work, we describe a molecular analysis of functional minimal domains responsible for specific DNA recognition and transcriptional activation (TAD1 and TAD2). We also define the importance of individual amino acids within the more important activation domain TAD1. Random mutagenesis at five amino acid positions showed the importance of acidic as well as hydrophobic residues within this minimal TAD. We also investigated the contribution of known general transcription factors and co-activators for Ino2-dependent gene activation. Although an ada5 single mutant and a gal11 paf1 double mutant were severely affected, a partial reduction in activation was found for gcn5 and srb2. Ino2 interacts physically with the basal transcription factor Sua7 (TFIIB of yeast). Interestingly, interaction is mediated by the HLH dimerization domain of Ino2 and by two non-overlapping domains within Sua7. Thus, Sua7 may compete with Ino4 for binding to the Ino2 activator, creating the possibility of positive and negative influence of Sua7 on ICRE-dependent gene expression.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Plant foods for human nutrition 13 (1966), S. 137-149 
    ISSN: 1573-9104
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Beschreibung / Inhaltsverzeichnis: Summary The Federal Institute for Agricultural Chemistry in Vienna has for a number of years investigated the mineral composition of different varieties of wheat and the influence of the nutritional status and other chemical factors of the soil on the minerals in the wheat plant. In addition, the mineral composition of the wheat plants has been investigated as to how it is dependent on the developmental state and growth of the plants. To this end it was necessary to use a point of reference which is constant during all of the growing period. Dry matter and ash are therefore not suitable for this purpose. Useful points of reference were found to be “the average plant”, the “total nutrient uptake per unit area” or the mineral content of the seed used. The investigations are by no means yet completed but some of the many data so far obtained, have been analysed. They concern not only the major but also a number of minor elements and they are presented in a summarized form. It can be seen that comparatively little information can be obtained from data relating the mineral content of the plants to their dry matter content and that luxury consumption and varietal differences of nutrient uptake and nutrient storage come to light, using the suggested points of reference.
    Kurzfassung: Résumé Depuis plusieurs années à la station fédérale de chimie agricole de Vienne des recherches sont en cours pour déterminer dans quelle mesure la teneur en substances minérales du blé depend des variétés, de la fumure et des facteurs agropédologiques. Les variations taux de substances minérales dans les diverses parties de la plante, pendant de la phase de la développement de la végétation, ont également été suivies. Il est nécessaire pour cela de rapporter les résultats à une valeur de référence qui reste constante durant toute la période de végétation. Le poids sec le taux de cendres, ne peuvent servir de référence. Il s'est révélé objectif de rapporter les résultats à la plantule moyenne, et de ramener les prélèvements de minéraux à la surface cultivée; le taux de substances minérales d'un grain de semence est aussi une référence utilisable. Nos recherches ne sont nullement terminées; une partie de nos très nombreux résultats numériques, qui se rapportent aussi bien aux macroéléments qu'à divers oligoéléments, ont été exploités et présentés de facon synthétique. L'information pauvre de la valeur relative des taux rapportés au poids sec apparaît ainsi; on peut mettre en évidence une consommation de luxe, ainsi qu'une différence dans le pouvoir d'absorption et de stockage selon la variété.
    Notizen: Zusammenfassung Seit mehreren Jahren sind an der Wiener Landw.-chemischen Bundesversuchsanstalt in Wien Untersuchungen im Gange, die zeigen sollen, ob und wie der Mineralstoffgehalt von Weizen sortenabhängig ist und wie der Mineralstoffgehalt verschiedener Sorten vom Düngungszustand und sonstigen bodenchemischen Faktoren abhängt. Auch wurde geprüft, wie sich der Mineralstoffgehalt in verschiedenen Teilen der Pflanze und in der ganzen Pflanze im Laufe der Vegetationsperiode verändert. Hierbei ist es notwendig, die Ergebnisse auf eine während der ganzen Vegetationszeit gleichbleibende Bezugsgröße zu beziehen. Trockengewicht oder Aschegehalt kommen daher nicht in Frage. Als geeignete Bezugsgrößen erwiesen sich die durchschnittliche Einzelpflanze, der Gesamtentzug pro Flächeneinheit, aber auch der Mineralstoffgehalt eines Saatkornes scheint brauchbar zu sein. Die Untersuchungen sind noch keineswegs abgeschlossen, ein Teil des vorhandenen außerordentlich umfangreichen Zahlenmaterials, das sich sowohl auf die Massenelemente als zum Teil auch auf die Spurenelemente bezieht, konnte aber schon ausgewertet werden und wird in übersichtlicher Form dargestellt. Der verhältnismäßig geringe Informationsgehalt der auf Trockensubstanz bezogenen Zahlen kommt hierbei deutlich zum Ausdruck und Luxuskonsum und sortenabhängiges Speicher- und Aufnahmevermögen werden deutlich.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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