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  • 1
    Digitale Medien
    Digitale Medien
    Assessment of the effect of molecular size on the electrophoretic mobility of humic substances (1996)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of soil science 47 (1996), S. 0 
    Details
    ISSN: 1365-2389
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The basic principles of the electrophoretic behaviour of humic substances (HS) still need to be systematically investigated. HS extracted from a Cryorthod (HS–1) and from an Haplumbrept (HS–2) were first fractionated by ultrafiltration to obtain two series of fractions of reduced polydispersity with a nominal mean relative molecular mass (M) of 7500, 20000, 40000, 75000 and 200 000 Da. Fractions extracted from the same soil behaved like an homologous series when tested by size exclusion chromatography, showing a linear relation of M with molecular size. When plotted against their mean M, the elution volumes of HS fractions extracted from the two different soils originated lines of different slope. Absolute electrophoretic mobilities of the fractions were determined in polyacrylamide gel slabs of increasing polyacrylamide concentration using a single buffer system; in all gels there was a close linear relation between the electrophoretic mobility and the logarithm of the mean M of HS fractions. Extrapolation of mobilities of HS–1 at zero gel concentration gave intercepts that did not differ significantly, showing that there was a constant mobility for all fractions in free solution. These results mean that charge differences have little effect on the electrophoretic mobilities of HS extracted from the same soil and imply the theoretical possibility of determining M distributions of HS by polyacrylamide gel electrophoresis in a single buffer system. Mobilities of HS–2 fractions were compared with those of HS–1: the latter fitted the regression equations of mobility against logarithm of the molecular weight obtained from HS–1 humic substances only in gels of small acrylamide concentration. Deviations were larger at small M, probably because of the increasing fulvic character of the fractions, and increased in gels of greater acrylamide concentration, indicating that charge differences may not be negligible when comparing humic substances extracted from different soils.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2389.1996.tb01393.x
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  • 2
    Digitale Medien
    Digitale Medien
    Fast bistable nematic display using monostable surface switching (1997)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 70 (1997), S. 1179-1181 
    Details
    ISSN: 1077-3118
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: We present a novel surface-controlled bistable nematic liquid crystal display. This black and white device uses simple monostable planar anchorings and a dielectrically positive nematic. The two bistable textures are, respectively, quasiuniform U and almost half-turn twisted T. Short electric pulses break the surface anchorings. Hydrodynamically coupled breaking of both anchorings creates T. U is obtained by breaking only one anchoring. Write and erase times for high resolution video are achieved at voltages compatible with the usual drivers.© 1997 American Institute of Physics.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1063/1.118479
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  • 3
    Digitale Medien
    Digitale Medien
    Capillary electrophoretic behaviour of humic substances from Sphagnum peats of various geographical origin: relation with the degree of decomposition (1998)
    Oxford, UK : Blackwell Science Ltd
    European journal of soil science 49 (1998), S. 0 
    Details
    ISSN: 1365-2389
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Humic substances (HS) extracted from 14 Sphagnum peats of various geographical origin were submitted tocapillary electrophoresis (CE) in free solution and in entangled polymer solutions (25 gl-1 and 50 gl−1) of polyethylene glycol (PEG). Electrophoretic runs were made in uncoated capillaries (75 μm internal diameter) using a tris-hydroxymethylaminomethane (TRIS)-phosphate buffer (pH 8.3) eventually containing PEG and applying a voltage of 10 kV. The HS extract of a Lithuanian peat was fractionated by ultrafiltration into five fractions with a nominal mean relative molecular mass (M) of 3000, 7500, 20 000, 75 000 and 200 000 Da. Without PEG, migration times were not correlated with the logarithm of M, but linear relations were obtained with 25gl−1 and 50gl−1 PEG solutions. Both solutions separated the different fractions by size; the best regression and the largest interval of linearity, M from 3000 to 200 000 Da, was obtained with the 25gl−1 PEG solution. At 10kV and without PEG, migration times ranged from 6.5 to 8.1 min and did not differentiate the peats, whereas at 25gl−1 PEG, migration times ranged from 3.3 to 6.5 min and, when plotted as a function of either the r value or the von Post index (H), which are used to evaluate the degree of decomposition of horticultural peats, they separated samples on the basis of their geographical origin and probable type of mire complex. At 25gl−1 PEG, migration times also showed a well-defined linear decreasing trend with the increase of mean annual temperatures. These relations suggest the hypothesis that humification in mires goes along with an increase in the average size of humic molecules.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2389.1998.4940589.x
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  • 4
    Digitale Medien
    Digitale Medien
    Activation of the carbonnickel σ-bond by cathodic reduction of trans-bromo-bis(triphenylphosphine)phenylnickel(II) in the presence of triphenylphosphine
    Amsterdam : Elsevier
    Inorganica Chimica Acta 42 (1980), S. 211-215 
    Details
    ISSN: 0020-1693
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/S0020-1693(00)88914-8
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  • 5
    Digitale Medien
    Digitale Medien
    A possible additional site of action for ketoconazole as a steroid synthesis inhibitor
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 28 (1987), S. 177 
    Details
    ISSN: 0022-4731
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(87)91615-3
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  • 6
    Digitale Medien
    Digitale Medien
    Effects of cyproterone acetate on steroid synthesis in metastatic adrenal carcinoma
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 28 (1987), S. 177 
    Details
    ISSN: 0022-4731
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(87)91616-5
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  • 7
    Digitale Medien
    Digitale Medien
    Vitamin D-25-hydroxylation in chronic liver disease
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 25 (1986), S. 72 
    Details
    ISSN: 0022-4731
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(86)90657-6
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  • 8
    Digitale Medien
    Digitale Medien
    Observations on the fate of oral 4-hydroxytryptophan in man (1961)
    Springer
    Cellular and molecular life sciences 17 (1961), S. 351-352 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Riassunto È stato studiato nell'uomo il destino del 4-idrossitriptofano (4-HTP) somministrato per bocca. Fra i metaboliti urinari dell'aminoacido quello di gran lunga più importante è risultato l'acido 4-idrossiindolacetico (10–12% riferito alDL-4-HTP). Scarsa è stata l'eliminazione urinaria di 4-HTP inalterato (1,3–2,2%) e ancora più scarsa quella della 4-idrossitriptamina (meno dell'1%). Un ulteriore importante metabolita del 4-HTP, presumibilmente un coniugato acido, è in corso di identificazione.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02201756
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  • 9
    Digitale Medien
    Digitale Medien
    Bioavailability and effects of heavy metals on soil microbial biomass survival during laboratory incubation (1995)
    Springer
    Biology and fertility of soils 19 (1995), S. 103-108 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Bioavailability ; CO2−C evolution ; Heavy metals ; Microbial biomass C ; Metabolic quotient ; Soil incubation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract In this work we studied the influence of Pb, Zn, and Tl on microbial biomass survival and activity during a laboratory incubation of soil. In comparison to uncontaminated soil, the microbial biomass C decreased sharply in soil contaminated with Zn and Tl, whereas the addition of Pb did not have any significant inhibitory effect on the level of microbial biomass C. Zn displayed the greatest biocidal effect, confirmed by the measurement of the death rate quotient (q D). The microbial activity, measured as CO2 evolution, increased significantly in contaminated soils, emphasizing the need of living organisms to expend more energy to survive. The greater demand for energy by microorganisms in order to cope with the toxicity of pollutants was also confirmed by measurement of the metabolic quotient (q CO2). In order to determine whether soil microorganisms affect the bioavailability of these metals through their mobilization and release, we studied the relationships between available Pb, Zn, and Tl, and microbial biomass C. The water-soluble fraction of Tl, available Tl, and Zn, and microbial biomass C were related significantly, but not Pb.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00336144
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  • 10
    Digitale Medien
    Digitale Medien
    Water- and pyrophosphate-extractable humic substances fractions as a source of iron for Fe-deficient cucumber plants (1997)
    Springer
    Biology and fertility of soils 26 (1997), S. 23-27 
    Details
    ISSN: 1432-0789
    Schlagwort(e): Key words Humic substances ; Cucumber ; Iron nutrition ; Iron deficiency ; Root acidification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract The capacity of Fe-deficient cucumber plants to utilise water-extractable and pyrophosphate-extractable humic substances as a source of Fe was investigated. Plants were grown for 13 days in nutrient solution in the presence or absence of Fe and during the last 7 days water-extractable and pyrophosphate-extractable humic substances were added to the solution at a final concentration of 5 μg organic C ml–1. The water-extractable humic fraction did not significantly modify leaf area and dry matter accumulation, leaf total Fe or chlorophyll content of cucumber plants adequately supplied with Fe. In contrast, pyrophosphate-extractable humic substances caused a slight but significant decrease of all the leaf parameters considered, with the exception of the chlorophyll content. Root Fe content of Fe-sufficient plants was decreased by more than 50% in the presence of each humified fraction. Addition of each humic fraction to Fe-deficient plants led to a partial disappearance of leaf chlorosis symptoms with a significant increase in chlorophyll and leaf Fe content. Fe content of roots was also significantly increased in Fe-deficient plants by the addition of humic substances to the nutrient solution. These results show that Fe-deficient cucumber plants can utilise Fe contained in the two fractions of humified organic matter. However, by calculating the amount of total Fe accumulated per plant in the presence of water-extractable or pyrophosphate-extractable humic substances, it could be seen that Fe contained in the water-extractable humic fraction was almost totally used by Fe-deficient cucumber plants, while that present in the pyrophosphate-extractable fraction could only be partially absorbed. The results strongly support a role of humified organic matter in Fe nutrition of plants and are discussed in terms of a possible interaction between soil humic substances and the biochemical mechanisms involved in the plant response to Fe deficiency.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s003740050337
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