Publication Date:
2011-02-19
Description:
Daily oscillations of gene expression underlie circadian behaviours in multicellular organisms. While attention has been focused on transcriptional and post-translational mechanisms, other post-transcriptional modes have been less clearly delineated. Here we report mutants of a novel Drosophila gene twenty-four (tyf) that show weak behavioural rhythms. Weak rhythms are accompanied by marked reductions in the levels of the clock protein Period (PER) as well as more modest effects on Timeless (TIM). Nonetheless, PER induction in pacemaker neurons can rescue tyf mutant rhythms. TYF associates with a 5'-cap-binding complex, poly(A)-binding protein (PABP), as well as per and tim transcripts. Furthermore, TYF activates reporter expression when tethered to reporter messenger RNA even in vitro. Taken together, these data indicate that TYF potently activates PER translation in pacemaker neurons to sustain robust rhythms, revealing a new and important role for translational control in the Drosophila circadian clock.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073513/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3073513/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lim, Chunghun -- Lee, Jongbin -- Choi, Changtaek -- Kilman, Valerie L -- Kim, Juwon -- Park, Sung Mi -- Jang, Sung Key -- Allada, Ravi -- Choe, Joonho -- R01 MH067870/MH/NIMH NIH HHS/ -- R01 MH067870-05/MH/NIMH NIH HHS/ -- R01 NS052903/NS/NINDS NIH HHS/ -- R01 NS052903-04/NS/NINDS NIH HHS/ -- R01 NS059042/NS/NINDS NIH HHS/ -- R01 NS059042-04/NS/NINDS NIH HHS/ -- R01MH067870/MH/NIMH NIH HHS/ -- R01NS052903/NS/NINDS NIH HHS/ -- R01NS059042/NS/NINDS NIH HHS/ -- England -- Nature. 2011 Feb 17;470(7334):399-403. doi: 10.1038/nature09728.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Neurobiology and Physiology, Northwestern University, Evanston, Illinois 60208, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/21331043" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Circadian Clocks/*genetics/physiology
;
Circadian Rhythm/genetics/physiology
;
Drosophila Proteins/biosynthesis/genetics/*metabolism
;
Drosophila melanogaster/*genetics/*physiology
;
Genes, Insect/*genetics
;
Genes, Reporter/genetics
;
Mutation/genetics
;
Neurons/metabolism/physiology
;
Period Circadian Proteins/*biosynthesis/genetics/metabolism
;
Poly(A)-Binding Proteins/metabolism
;
Protein Binding
;
Protein Biosynthesis/genetics/*physiology
;
RNA, Messenger/genetics/metabolism
;
Up-Regulation
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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