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  • 11
    ISSN: 1617-4623
    Keywords: Key wordsNeurospora crassa ; Mitochondria ; Complex I ; Assembly ; Gene disruption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nuclear gene coding for the 20.8k-kDa subunit of the membrane arm of respiratory chain NADH:ubiquinone reductase (Complex I) from Neurospora crassa, nuo-20.8 was localized on linkage group I of the fungal genome. A genomic DNA fragment containing this gene was cloned and a duplication was created in a strain of N. crassa by transformation. To generate RIP (repeat-induced point) mutations in the duplicated sequence, the transformant was crossed with another strain carrying an auxotrophic marker on chromosome I. To increase the chance of finding an isolate with a non-functional nuo-20.8 gene, random progeny from the cross were selected against this auxotrophy since RIP of the target gene will only occur in the nucleus carrying the duplication. Among these, we isolated and characterised a mutant strain that lacks the 20.8 kDa mitochondrial protein, indicating that this cysteine-rich polypeptide is not essential. Nevertheless, the absence of the 20.8-kDa subunit prevents the full assembly of complex I. It appears that the peripheral arm and two intermediates of the membrane arm of the enzyme are still formed in the mutant mitochondria. The NADH:ubiquinone reductase activity of sonicated mitochondria from the mutant is rotenone insensitive. Electron microscopy of mutant mitochondria does not reveal any alteration in the structure or numbers of the organelles.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 25 (1969), S. 141-142 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Les auteurs étudient le contenu en ATP chez leStreptococcus faecalis, après l'addition de glucose et en présence de diverses substances. Les effets les plus évidents ont été ceux du dicumarol augmentant la valeur de la constante d'entrée et la quantité de l'ATP; du 2,4-dinitrophénol augmentant les 2 constantes (d'entrée et de décomposition); de l'arsénate réduisant la quantité de l'ATP; de l'azide augmentant la constante de décomposition; et de Ja gramicidine qui augmente non seulement la constante de décomposition mais réduit aussi la quantité de l'ATP dans le «pool».
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 10 (1954), S. 187-188 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé Le monoïodoacetate de sodium injecté à des poulets par voie intraveineuse en une dose unique de 40 mg/kg, provoque des lésions très précoces dans toutes les couches cellulaires de la rétine. Une dégénérescence plus ou moins complète survient 6 à 7 jours après l'injection. On peut conclure que le poulet présente une sensibilité au monoïodoacetate plus grande que celle des mammifères precédemment étudiés (lapin, chat, macaque).
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 17 (1961), S. 270-271 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Die Verarmung von Glykogen in der Leber als Ausdruck der toxischen Wirkung von Thioacetamid kann durch Hypophysektomie oder Thyroidektomie verhindert werden.
    Type of Medium: Electronic Resource
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 25 (1969), S. 249-250 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Résumé L'activité ATPasique après l'addition d'ATP et en présence de diverses substances est étudié chezStreptococcus faecalis. Les effets les plus évidents ont été les effects inhibiteurs de l'acide sodique et de la gramicidine.
    Type of Medium: Electronic Resource
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  • 16
    ISSN: 1349-9432
    Keywords: image classification ; random image field ; power spectrum ; synthetic discriminant function ; statistical estimation ; Fourier-optical technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The problem of classification of images that have a perfectly random nature is considered. We propose a new approach to solve this problem that is based on the use of the synthetic discriminant functions being synthesized to separate linearly the power spectra of random image fields to be classified. In the stage of both discriminant function synthesis and classification, the statistical technique of power spectrum estimation is employed. The realization of the proposed approach by means of a hybrid computer-optical technique is discussed, and its efficiency is illustrated by two examples of real-world texture classification.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 27 (1975), S. 145-149 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The possibility of linkage was tested in 3 large kindreds with Waardenburg syndrome type I against the AB0 locus. Loose linkage is probably present; the recombination fraction in males, females, and both sexes combined seems to be approximately $$\widehat{\theta '} = 0.175$$ , $$\hat \theta = 0.255$$ , and $$\widehat{\theta ',\theta } = 0.20$$ , respectively. There are still more informative matings to be studied in those pedigrees, as well as the inminent possibility of determining the phase status in several of them, for the AB0 locus and a few other loci.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 96 (1998), S. 621-627 
    ISSN: 1432-2242
    Keywords: Key words Cacao ; Theobroma cacao ; Genetic diversity ; Crop evolution ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Neotropical tree crops are affected by a combination of biological and human factors that complicate the study of genetic diversity and crop evolution. Genetic diversity and relationships among southern Mexican populations and horticultural collections of Theobroma cacao (chocolate, cocoa, cacao) are examined in light of the agricultural practices of the Maya. Collections of cacao were obtained from the extremes of its geographic range including archeological sites in southern Mexico where cacao was first domesticated. Genetic diversity was assayed by 57 informative random amplified polymorphic DNA (RAPD) marker loci. A unique sample of the total diversity found in this study exists in the southern Mexican populations. These populations are significantly different from all other cacao with regards to their profile of RAPD bands, including the ‘criollo’ variety, their morphological and geographical group. A population of cacao found in a sinkhole (cenote) in northern Yucatan with genetic affinities to populations in Chiapas suggests the Maya maintained plants far away from their native habitat. This finding concurs with known agroforestry practices of the Maya. Modern efforts to increase germplasm of tropical tree crops such as cacao should carefully examine archeological sites where genetic diversity, either deliberately or by chance, was collected and maintained by ancient cultures.
    Type of Medium: Electronic Resource
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical chemistry accounts 72 (1987), S. 325-331 
    ISSN: 1432-2234
    Keywords: Electron affinity ; MBPT-MP2-CIPSI
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Using a multireferent MBPT method (CIPSI) the electronic affinity (EA) of F, CN and HCC is computed. Results show how UMP2 gives unbalanced truncation of the MP series, while ROMP2 has the correct (balanced) behaviour. The good agreement with the experimental EA found for some compounds is accidental and associated to an error compensation. The good agreement with the experimental data found for the ROMP2 and CIPSI EAs is analysed.
    Type of Medium: Electronic Resource
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    The European physical journal 328 (1987), S. 177-187 
    ISSN: 1434-601X
    Keywords: 21.60Jz ; 21.10.Re ; 24.30.Cz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We derive the markovian limit of the collisional Random Phase Approximation (CRPA) by resort to the linearization of the Collisional Time-Dependent-Hartree-Fock (CTDHF) equation of motion for the one-body density matrix. The CRPA spectral problem is numerically solved in the frame of a model consisting of a finite fermion system with axial symmetry interacting by means of a separable force. Calculations are performed within a range of interaction strengths, temperature and size parameters and it is shown that both the centroids and the widths of the high energy collective modes exhibit the trend of experimental data.
    Type of Medium: Electronic Resource
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