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  • 11
    Electronic Resource
    Electronic Resource
    Improvement scheme for the determination of arsenic species in mussel and fish tissues (1999)
    Springer
    Fresenius' journal of analytical chemistry 363 (1999), S. 5-11 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Six interlaboratory studies were organised by the Standard, Measurement and Testing Programme of the European Commission on the determination of arsenic species (arsenobetaine, arsenocholine, monomethylarsonic acid, dimethylarsinic acid, As(III) and As(V)) in marine matrices and soil. A step-by-step approach was used and a meeting was held at the end of each study to help the participants to discover errors and to improve their analytical methods. The successive steps investigated the calibration procedures on various solutions, the separation and derivatisation techniques on solutions and extracts and the extraction on mussel and fish tissues. All materials used for the study were monitored for their stability. Verified calibration solutions and compounds were distributed to the participants in each exercise in order to trace calibration problems. The agreement between the results improved regularly and at the end of the six campaigns allowed the certification of a reference material of tuna-fish tissue (BCR-CRM 627) for its total arsenic, arsenobetaine and dimethylarsinic acid contents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051129
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  • 12
    Electronic Resource
    Electronic Resource
    Preparation of pure calibrants (arsenobetaine and arsenocholine) for arsenic speciation studies and certification of an arsenobetaine solution – CRM 626 (1999)
    Springer
    Fresenius' journal of analytical chemistry 363 (1999), S. 12-17 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Interlaboratory studies have been organised by the Standards, Measurement and Testing Programme in order to certify the content of some chemical forms of arsenic in fish and mussel tissue, soil and sediment. Among the six compounds of interest (arsenite, arsenate, dimethylarsinic and monomethylarsonic acids, arsenobetaine and arsenocholine) only four are commercially available. Arsenobetaine and arsenocholine of well defined purity had therefore to be prepared by the pilot laboratories in charge of the management of the project. The present work deals with the synthesis and characterisation of these two compounds and the certification of the content of arsenobetaine in a standard solution (BCR-CRM 626).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051130
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  • 13
    Electronic Resource
    Electronic Resource
    Certification of total arsenic, dimethylarsinic acid and arsenobetaine contents in a tuna fish powder (BCR-CRM 627) (1999)
    Springer
    Fresenius' journal of analytical chemistry 363 (1999), S. 18-22 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Tuna fishes were collected in the Straits of Messina (Italy), were dissected and dorsal muscles minced, freeze-dried, ground and sieved. The obtained powder was stabilised by γ-irradiation and filled into brown glass bottles. The homogeneity and stability at +20 °C and +40 °C were verified with regards to the total arsenic, dimethylarsinic acid and arsenobetaine contents. Ten laboratories participated in the certification study. All participants had demonstrated beforehand their ability to produce accurate results for the As speciation in fish tissue. The certified values are: total arsenic (4.8 ± 0.3) mg/kg, arsenobetaine (52 ± 3) μmol/kg, dimethylarsinic acid (2.0 ± 0.3) μmol/kg. The material is available from the BCR since early 1998.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160051131
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  • 14
    Electronic Resource
    Electronic Resource
    Certification of the contents of five polychlorodibenzo-p-dioxins and six polychlorodibenzo furans in milk powder (1997)
    Springer
    Fresenius' journal of analytical chemistry 359 (1997), S. 222-229 
    Details
    ISSN: 1432-1130
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  Preparation, homogeneity and stability studies of a natural milk powder material and subsequent certification of the mass fraction of five polychlorodi-benzo-p-dioxins (D48, D54, D66, D67, D70) and six polychlorodibenzo furans (F83, F94, F114, F118, F121, F130) are described. The material, CRM 607, is intended for use in the method validation and quality control of the complete analytical procedure for congener-specific determination of dioxins in milk powder and similar dairy products and was certified in an interlaboratory study for: 2,3,7,8-TCDD, 0.25 ng/kg; 1,2,3,7,8-PeCDD, 0.79 ng/kg; 1,2,3,4,7,8-HxCDD 0.42 ng/kg; 1,2,3,6,7,8-HxCDD, 0.98 ng/kg; 1,2,3,7,8,9-HxCDD, 0.34 ng/kg; 2,3,7,8-TCDF, 0.05 ng/kg; 1,2,3, 7,8-PeCDF, 0.054 ng/kg; 2,3,4,7,8-PeCDF, 1.81 ng/kg; 1,2,3,4,7, 8-HxCDF, 0.94 ng/kg; 1,2,3,6,7,8-HxCDF, 1.01 ng/kg and 2,3,4,6,7,8-HxCDF, 1.07 ng/kg. An indicative (non-certified) value was assigned to the mass fraction of the hepta- and octachlorinated congeners D73, D75, F131, F134, F135 and the HxCDF F124.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002160050564
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  • 15
    Electronic Resource
    Electronic Resource
    In vivo and in vitro studies on interactions between the components of the hemolysin (HlyA) secretion machinery of Escherichia coli (1997)
    Springer
    Molecular genetics and genomics 256 (1997), S. 306-319 
    Details
    ISSN: 1617-4623
    Keywords: Key words Hemolysin secretion ; HlyD mutants ; HlyD/HlyB suppressor mutants ; TolC ; Vancomycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The glycopeptide antibiotic vancomycin blocks cell wall synthesis in Escherichia coli only when it can reach its target site in the periplasm. In vivo, sensitivity to vancomycin is enhanced in the presence of the hemolysin (hly) determinant of E. coli or its translocator portion hlyBD. Two different mutations in hlyD alter the cell's susceptibility to vancomycin: mutations in the tolC-homologous region of hlyD increase vancomycin resistance, whereas mutations at the 3′-terminus of hlyD lead to hypersensitivity to vancomycin and to the accumulation of large periplasmic and cytoplasmic pools of this antibiotic in E. coli. These effects are only observed in the presence of functional HlyB and TolC, the two other components of the hemolysin secretion machinery. A defect in TolC causes hyperresistance to vancomycin, even when present together with a mutant HlyD protein which in the presence of TolC renders E. coli hypersensitive to vancomycin. Lipid bilayer experiments in vitro revealed specific interactions between TolC and vancomycin or HlyD protein. Second-site suppressor mutations in hlyD and hlyB were obtained, which abolish the hypersensitive phenotype caused by the 3′-terminal mutations in hlyD. Our results are compatible with the idea that (a) TolC, together with the TolC-homologous part of HlyD, forms a pore in the outer membrane through which hemolysin is released and vancomycin taken up; and (b) the C-terminal sequence of HlyD interacts with periplasmic loop(s) of HlyB to form a closed channel spanning the periplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050574
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  • 16
    Electronic Resource
    Electronic Resource
    To deal with the „Invisible” (1993)
    Springer
    Naturwissenschaften 80 (1993), S. 476-478 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01136043
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  • 17
    Electronic Resource
    Electronic Resource
    The spectral sensitivity of a passerine bird is highest in the UV (1989)
    Springer
    Naturwissenschaften 76 (1989), S. 82-83 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396716
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  • 18
    Electronic Resource
    Electronic Resource
    DNA content of haploid and diploid nuclei in a male XO type bug (1983)
    Springer
    Cellular and molecular life sciences 39 (1983), S. 417-418 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The DNA content of sperm, spermatide, and brain cell nuclei was determined by Feulgen microspectrophotometry inDysdercus intermedius. Both in haploid and diploid cells 2 classes of DNA content were found. The differences are due to a large X chromosome in this male XO type bug. The absolute amounts of DNA represented by the X chromosome and the complete genome were calculated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01963157
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  • 19
    Electronic Resource
    Electronic Resource
    Theorie des muldenförmigen Verschleißes im Blasversatzrohr (1953)
    Springer
    Archive of applied mechanics 21 (1953), S. 382-395 
    Details
    ISSN: 1432-0681
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00535862
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  • 20
    Electronic Resource
    Electronic Resource
    Energy dispersive X-ray fluorescence as a tool in biomedical analysis (1988)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 331 (1988), S. 58-60 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The energy dispersive X-ray fluorescence spectrometer (EDXRF) developped together with the Siemens Company (FRG) allows to perform rapid multi-elemental analysis of biological fluids without pretreatment of the samples. The performance of this technique and its features are reported in two different fields: blood analysis and pulmonary investigations. Blood and plasma analysis were performed for more than 2500 patients and a statistical treatment of the data lead to the normal concentrations of trace elements Se, Rb, Cu, Zn, Fe, Ca, Br, K. Toxic elements such as Hg or Pb and also drugs like Au and Pt compounds can be detected even after a long period after treatment. To illustrate the performance of the spectrometer we report the influence of Cis-Pt treatment on the trace element concentrations in plasma of patients with anemia. The EDXRF spectrometry was also used to investigate the inorganic composition of the broncho-alveolar lavage fluids. After determination of the normal concentration range, it was possible to characterize some typical pulmonary diseases due to occupational exposure. Some examples of detected pneumoconiosis and for some patients the presence in the alveoli of elements due to special medication (Amiodarone, Aurothiopropanol-sulfonate) are mentioned. Other fields are being investigated such as: follow-up of Mn dependent enzymes in neuronal and glial cells in culture; origin of lead in dental tissues or composition of lyophilized food powders. They will be briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00473898
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