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  • American Institute of Physics (AIP)  (20)
  • International Union of Crystallography (IUCr)  (11)
  • 11
    Electronic Resource
    Electronic Resource
    Direct-method-aided phasing of MAD data (2001)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 57 (2001), S. 250-253 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: The direct methods of breaking the phase ambiguity intrinsic in one-wavelength anomalous scattering (OAS) data and MAD phasing are powerful methods in their own rights. In a different context, in addition to their success in phasing OAS data, direct methods can also be useful in the treatment of MAD data. The idea has been tested with the MAD data at 2.5 Å resolution from the protein human adenosine kinase [Mathews et al. (1998), Biochemistry, 37, 15607–15620]. The results showed that the incorporation of direct methods in MAD phasing led to a significant improvement of phases over those obtained from the conventional MAD phasing method alone, as indicated by improved map correlation coefficients (with the existing model), reduced phase errors by 4.5° and improved map connectivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444900018606
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  • 12
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray diffraction studies of recombinant staphylokinase (1996)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 564-565 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Staphylokinase, a fibrin-specific plasminogen activator, was highly expressed in Escherichia coli and purified by ion-exchange and gel-filtration chromatography. The purified recombinant staphylokinase was fully active and readily crystallized against 1.2 M sodium citrate in 100 mM Tris–HCl buffer at pH 8.0 using the hanging-drop method. Crystals of staphylokinase diffract to better than 2.2 Å resolution. The crystal belongs to the tetragonal space group P41212 or its enantiomorph with unit-cell parameters a = b = 67.5, c = 150.1 Å. There are two molecules in the asymmetric unit. In this paper, we described the first crystallization of a kind of plasminogen activator and present the results of preliminary X-ray diffraction data from the native protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444995013552
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  • 13
    Electronic Resource
    Electronic Resource
    Solving crystal structures from two-wavelength X-ray powder diffraction data – breaking the phase ambiguity in the noncentrosymmetric case (2000)
    [S.l.] : International Union of Crystallography (IUCr)
    Acta crystallographica 56 (2000), S. 592-595 
    Details
    ISSN: 1600-5724
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Direct methods of breaking phase ambiguities in protein crystallography have been introduced in powder diffraction analysis. This is aiming at ab initio solution of noncentrosymmetric structures using two-wavelength anomalous powder diffraction data. The known structure of the hydrogen bromide salt of leotidine (C14H20O2N2·HBr) in space group P212121 was used for simulating two-wavelength anomalous powder diffraction with the Br atom as anomalous scatterer. X-ray wavelengths are selected at \lambda_1 = 0.920 and \lambda_2 = 1.500 Å. Unique reflections from the diffraction pattern of \lambda_2 were able to locate the Br atom accurately. All overlapping diffraction peaks were uniformly partitioned to decompose into single reflections. Structure-factor amplitudes were then extracted. With these and the substructure of Br atoms, unique phases for centric reflections (hk0, h0l and 0kl) and phase doublets for noncentric reflections were obtained. The direct method was used to break the phase ambiguity leading to an interpretable electron-density map, from which five cycles of Fourier iteration yielded the complete structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0108767300011417
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  • 14
    Electronic Resource
    Electronic Resource
    Photoelectron spectroscopy of the multiphoton ionization and above threshold ionization of HI at 532 and 355 nm (1994)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 101 (1994), S. 86-94 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Multiphoton ionization and fragmentation of the hydrogen iodide molecule have been examined at 355 and 532 nm and optical field intensities of 1011−5×1012 W cm−2 by photoelectron spectroscopy and time-of-flight mass spectrometry. At 532 nm, scission of the H–I bond is favored over direct photoionization of the molecule. Three and four photon ionization of HI at 355 nm ((h-dash-bar)ω≈3.5 eV), however, reveals clear vibrational progressions associated with the HI+ 2Π3/2 and 2Π1/2 ion product states. Analysis of these spectra yields vibrational frequencies for the 2Π3/2 and 2Π1/2 states that agree to better than 8% with those determined by Böwering et al. [Chem. Phys. Lett. 189, 467 (1992)] from single photon ionization experiments. Above threshold ionization (ATI) of both HI and atomic iodine are also observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.468490
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  • 15
    Electronic Resource
    Electronic Resource
    Magnetic properties of Fe/Pd multilayers grown by electron-beam evaporation (1995)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 77 (1995), S. 3965-3970 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The magnetic properties of [Fe(20 A(ring))/Pd(x A(ring))]25 multilayers prepared by ultrahigh vacuum electron-beam evaporation are presented. It is found that the crystal structure of Fe layers change from bcc to fcc when the thickness of Pd layers dPd≥36 A(ring). The saturation magnetization per unit Fe volume at 5 K is enhanced and oscillates with the increment of the thickness of Pd layers, due to the polarization of Pd atoms. The magnetic hysteresis loops of samples indicate low coercive forces, and ferromagnetic coupling between the Fe layers for all Pd thicknesses (6–60 A(ring)). The conversion electron Mössbauer spectra measurements proved that the magnetic moment of fcc Fe is the same as that of the bcc Fe. It is also found that the magnetic anisotropy dependence on dPd is similar to that of the saturation magnetization, and relates to the Fe layer structure transition. The Curie temperature of Fe/Pd multilayers decreases monotonously with the increasing of dPd. The low temperature magnetization measurement of Fe/Pd multilayers suggests that the interlayer coupling between Fe layers and polarization of Pd layers influences the temperature dependence of saturation magnetization. No evidence of antiferromagnetic coupling between Fe layers and giant magnetoresistance effect is found. The relationship among structure, polarization of Pd layers, and magnetic coupling is discussed. © 1995 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.358579
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  • 16
    Electronic Resource
    Electronic Resource
    Determination of surface roughness of InP (001) wafers by x-ray scattering (1994)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 76 (1994), S. 4154-4158 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: The surface roughness of polished InP (001) wafers were examined by x-ray reflectivity and crystal truncation rod (CTR) measurements. The root-mean-square roughness and the lateral correlation scale were obtained by both methods. The scattering intensities in the scans transverse to the specular reflection rod were found to contain two components. A simple surface model of surface faceting is proposed to explain the experimental data. The sensitivities of the two methods to the surface structure and the role of the resolution functions in the CTR measurements are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.357366
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  • 17
    Electronic Resource
    Electronic Resource
    Granular magnetoresistance ribbon material suitable for isotropic magnetic field transducers (1994)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 76 (1994), S. 4918-4920 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Magnetic granular CoCu alloy ribbons were prepared by the melt-spun method, and the structural, magnetotransport, magnetic properties, and the effects of annealing on these properties were studied by x-ray diffraction, vibrating sample magnetometer, and magnetoresistance (MR) measurements at room temperature. Giant magnetoresistance was observed over a broad range of Co concentrations with a maximum MR ratio of 7.8% (at x=0.20 and 500 °C annealing) under a magnetic field of 20 kOe at room temperature. It is found that the relationship between MR and magnetic field H depended strongly on the annealing temperature and a linear MR-H relation could be obtained at an optimum annealing condition. The MR ratio did not depend on the direction between H and electric current I. These features are very suitable for making isotropic magnetic field transducers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.358442
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  • 18
    Electronic Resource
    Electronic Resource
    e-beam probe potential diagnostic in spherical electrostatic-inertial plasma confinement (SEIC) (abstract) : Proceedings of the 9th topical conference on high temperature plasma diagnostics (1992)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 63 (1992), S. 4731-4731 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A new e-beam probe is developed to study the potential structure inside a SEIC device [G. H. Miley et al., Fusion Technol. 19, 840 (1991)]. The device has a spherical grid negatively biased inside a spherical vacuum chamber. Ions oscillate through the highly transparent grid, forming a single potential well inside the cathode. The potential in turn attracts electrons from ionization of background gas. The measurement of this complex potential profile is the objective of this work. The e-beam probe offers advantages over the e-emitting probes and the laser heterodyne method. It minimizes perturbations (versus a physical probe) and detects smaller charge density variations than possible with laser techniques. The present e-beam probe utilizes a higher voltage and a modified focus compared to the earlier version used by Swanson [Swanson et al., Phys. Fluids 16, 1939 (1973)]. The characteristic e-beam deflection patterns observed are compared to predictions from an impulse approximation model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1143622
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  • 19
    Electronic Resource
    Electronic Resource
    Potential profile measurements using a collimated proton detector in spherical inertial-electrostatic plasma confinement : Proceedings of the 9th topical conference on high temperature plasma diagnostics (1992)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 63 (1992), S. 4810-4812 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: A collimated proton detector has been developed for spatial resolved proton measurements in inertial-electrostatic plasma confinement (IEC) fusion experiments. These are the first proton measurements used to infer potential well profiles on an IEC device. This paper describes a new technique for investigating the existence of multiple potential wells inside IEC devices. Analysis of the observed proton energy and source profile indicates that (for a 12-mA cathode current, a 30-kV cathode voltage in a 4-mTorr D2 background) predominantly beam-background fusion occurs. Computer simulation suggests that a positive space charge potential approximately half that of the applied voltage is formed inside the cathode. These results establish the first measurement of a positive potential well structure inside an ion-injected IEC device.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1143844
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  • 20
    Electronic Resource
    Electronic Resource
    Biased probe for plasma diagnostics in spherical electrostatic inertial plasma confinement : Eight topical conference on high−temperature plasma diagnostics (1990)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 61 (1990), S. 2902-2904 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: The biased probe, as a unique extension of the Langmuir probe, offers several advantages in measuring the charged-particle flux versus position in a beam-dominated plasma such as encountered in the spherical electrostatic inertial confinement (SEIC) device. The probe operates in a non-Maxwellian and locally non-neutral plasma, where the Debye length is large compared to the probe itself. Its conical particle acceptance extension minimizes the measurement error caused by the distortion of the SEIC potential structures. Either the ion or electron flux can be measured using the proper bias on its inner collecting element.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1141768
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