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  • 11
    Digitale Medien
    Digitale Medien
    The Ultrastructure of the Cyst Wall of Giardia lamblia (2004)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 51 (2004), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Giardiasis is the most common human protozoal infection. In then: cystic phase, giardias are protected from the environment by a filamentous cyst wall made up of carbohydrates, proteins, and by two outer membranes separated from the plasma membrane of the parasite by a peripheral space. The present transmission electron microscope observations of G. lamblia cysts of human origin suggest that the extracellular peritrophic space originates from the growth, elongation, and fusion of large cytoplasmic vacuoles. As the large clear vacuoles grew in size, flattening against the inner face of the plasma membrane, they formed a single vacuole that surrounded the body of the parasite, eventually forming two outer membranes. In mature Giardia cysts, the original plasma membrane of the trophozoite becomes the outermost membrane of the cyst wall (CM1). The large vacuoles form a second membrane surrounding the cyst (CM2), and also form a third membrane (CM3), that becomes the new plasma membrane of the trophozoite. During excystation CM1 and CM2 attach to each other and fragment, leaving abundant membrane residues in the peritrophic space. Knowledge of the biochemical composition and functional properties of the complex outer membranous system of G. lamblia cysts here described will be of use to understand the survival of Giardia cysts in the environment, a major factor responsible for the high prevalence of giardiasis worldwide.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2004.tb00549.x
    Standort Signatur Erwartet Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    Molecular Characterization of Cryptosporidium sp. from Animals in Spain (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00626.x
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Two Trichomonas vaginalis Loci Encoding for Distinct Cysteine Proteinases Show a Genomic Linkage with Putative Inositol Hexakisphosphate Kinase (IP6K2) or an ABC Transporter Gene (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00695.x
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  • 14
    Digitale Medien
    Digitale Medien
    Metacystis borrori n. sp. (Ciliophora: Metacystidae) on the Seagrass Thalassia testudinum (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . A new epibiontic ciliate of the genus Metacystis is described on the seagrass Thalassia testudinum of the coral reef lagoons of Veracruz, Mexico. The ciliate was studied in living and stained specimens and under the scanning electron microscope. The cell body (10–35 × 10–18 μm in vivo) is transversely annulated (4–6 rings). The somatic ciliature consists of 22–30 longitudinal kineties, and patterned as 5–7 transverse kinetics. The circumoral kinety is composed of kinetosomes closely spaced. The macronucleus diameter measures about 3–7 μm. The lorica (18–61 × 11–26 μm) has the posterior end round to conical or irregular with mucoid filaments. This prostomatid colonizes both natural and artificial substrates placed in an aquarium. Metacystis borrori n. sp. is a species that forms part of the ciliate community on Thalassia testudinum with a temperature range of 21–26°C and a salinity of 32–40‰.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00118.x
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  • 15
    Digitale Medien
    Digitale Medien
    An Extracellular monoADP-ribosyl Transferase Activity in Entamoeba histolytica Trophozoites (2002)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 49 (2002), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Due to the important role of monoADP-ribosyl transferases in physiological and pathological events, we investigated whether the protozoan parasite Entamoeba histolytica had monoADP-ribosyl transferase activity. Reactions were initiated using amebafree medium as the source of both enzyme and ADP-ribosylation substrate(s) and [32P]NAD+ as source of ADP-ribose. Proteins were analyzed by electrophoresis, and [32P]-labeled proteins were detected by autoradiography. Using the crude extracellular medium, a major labeled product of Mr 37,000 was observed. The yield of this product was reduced markedly using medium from Brefeldin A-treated trophozoites, indicating that the extracellular monoADP-ribosyl transferase and/or its substrate depended on vesicular transport. The labeling of the 37-kDa substrate was dependent on reaction time, temperature, pH, and the ratio of unlabeled NAD+ to [32P]NAD+. After two purification steps, several new substrates were observed, perhaps due to their enrichment. The reaction measured ADP-ribosylation since [14C-carbonyl]NAD+ was not incorporated into ameba substrates and a 75-fold molar excess of ADP-ribose caused no detectable inhibition of the monoADP-ribosyl transferase reaction. On the basis of sensitivity to NH2OH, the extracellular monoADP-ribosyl transferase of E. histolytica may be an arginine-specific enzyme. These results demonstrate the existence in E. histolytica of at least one extracellular monoADP-ribosyl transferase, whose localization depends upon a secretion process.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2002.tb00228.x
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  • 16
    Digitale Medien
    Digitale Medien
    Trichomonas vaginalis: In Vitro Attachment and Internalization of HIV-1 and HIV-1-Infected Lymphocytes (2003)
    Oxford, UK : Blackwell Publishing Ltd
    The @journal of eukaryotic microbiology 50 (2003), S. 0 
    Details
    ISSN: 1550-7408
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: . Sexually transmitted diseases (STDs) caused by bacteria and protozoa play an important role in the epidemiology of human immunodeficiency virus (HIV-1) infection. Human trichomoniasis, produced by the protozoan parasite Trichomonas vaginalis, is one of the most common STDs, and is a cause of mucosal lesions in the urogenital tract, which may increase the risk for HIV infection. However, there are no reports concerning the outcome of in vitro interactions between HIV particles and trichomonads. Therefore, we incubated T. vaginalis with three subtypes of HIV-1 (A, B, and D), as well as with HIV-1-infected lymphocytes, and analyzed the interactions with immunofluorescence microscopy and transmission electron microscopy. Our results demonstrated that HIV-1 particles attach and are incorporated into T. vaginalis through endocytic vesicles and are degraded within cytoplasmic vacuoles in approximately 48 h. There was no ultrastructural evidence of HIV-1 replication in trichomonads. These results demonstrated that trichomonads may internalize and harbor HIV-1 particles for short periods of time. In addition, under in vitro conditions, T. vaginalis ingests and digests HIV-1-infected lymphocytes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1550-7408.2003.tb00104.x
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  • 17
    Digitale Medien
    Digitale Medien
    Novel variation for the tocopherol profile in a sunflower created by mutagenesis and recombination (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: α-Tocopherol is the main tocopherol in sunflower seeds (〉90%). Because it exerts a weak antioxidant action in vitro, its partial replacement by other tocopherols is an important breeding objective in this crop. The objective of this research was to develop novel tocopherol profiles in sunflower through mutagenesis and genetic recombination. Seeds of four ‘Peredovik’ accessions were used for chemical mutagenesis with ethyl methane sulfonate (EMS). Single-seed screening in the M2 generation resulted in two M2 seeds, derived from different M1 plants, with increased γ-tocopherol contents of 19.2% and 96.7%, respectively. M3 progeny from the M2 seed with the 96.7% content bred true for high c-tocopherol content, containing more than 90%γ-tocopherol. M3 progeny from the M2 seed with only 19.2%γ-tocopherol segregated in a range from 0 to 84.6%. Selection for high c-tocopherol content produced an M4:5 line, designated IAST-1, with a stable high concentration of γ-tocopherol. Crosses between IAST-1 and T589, with an increased b-tocopherol content, produced F2 segregants with trans-gressive levels of up to 77%β-tocopherol or up to 68% d-tocopherol. Both novel tocopherol profiles were confirmed in the F3 generation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.01012.x
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  • 18
    Digitale Medien
    Digitale Medien
    Breeding for resistance to begomovirus in tropic-adapted tomato genotypes (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Four tomato lines introgressed from Lycopersicon chilense were compared with the commercial F1 hybrids ‘ARO 8479’ and ‘HA 3108’, which are tolerant to Tomato yellow leaf curl virus, and the cv. ‘Campbell 28’ as a susceptible control. Resistance was evaluated by the use of grafted diseased scions as well as in a field trial where plants infected by viruliferous whiteflies and disease-free plants were transplanted in paired rows. The new lines LD 3, LD 4, LD 5 and LD 6 showed no disease symptoms after grafting or in the field trial. Virus accumulation at 60 days after transplanting was low in the infected plants: 0.09, 0.60, 1.00 and 0.50 ng, respectively. No fruit-set or yield losses were registered under the high temperature conditions prevalent in the trial, in which lines LD 5 and LD 6 were better adapted to tropical conditions. Viral DNA concentrations were over 1000 ng in the cvs.‘Campbell 28′,‘ARO 8479’ and ‘HA 3108’. The last two are considered tolerant as they were asymptomatic or had mild symptoms, respectively, but achieved acceptable yields in the trial. By contrast, virus had a negative effect on fruit-set, number of fruit per plant and total yield in the cv.‘Campbell 28’.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00959.x
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  • 19
    Digitale Medien
    Digitale Medien
    1-MCP Preserves the Firmness of Stored Persimmon ‘Rojo Brillante’ (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: : We studied the effects of 1-methylcyclopropene (1-MCP), an ethylene inhibitor, on quality of persimmon cv.‘Rojo Brillante.’Two assays were carried out, applying deastringency treatment before or after storage at 15 °C. In both experiments, fruit was treated with 0 (control), 0.5, or 1 μL/L of 1-MCP after harvest. Additionally, the effect of 2 applications of 0.5 μ L/L, one after harvest and the 2nd after a storage period, was evaluated. In all experiments, 1-MCP delayed softening and color evolution of persimmons. Ethanol and acetaldehyde levels in treated fruit were lower than those in control fruit. 1-MCP had no effects on weight loss, °Brix, or ethylene and CO2 production.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb15516.x
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  • 20
    Digitale Medien
    Digitale Medien
    Improving the Nutritive Value of Homogenized Infant Foods Using Response Surface Methodology (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 69 (2004), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: : Response surface methodology was used to develop infant foods with a goal of achieving low amounts of antinutritive substances and high trace element content. The effects detected highlight the importance of both the amount and the proportion of each ingredient. For example, whereas carrot exerted a strong negative effect (-5.18) on soluble dietary fiber content in chicken with rice (CR) beikost, its effect was positive in the veal with carrot (VC) beikost (2.51). Similarly, contrasting results were found for the effect of carrot on phytic acid content. Liver had a particularly marked effect on Cu, Zn, and Fe content in the CR beikost and on Cu in the VC beikost.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.2004.tb17884.x
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