ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

feed icon rss

Ihre E-Mail wurde erfolgreich gesendet. Bitte prüfen Sie Ihren Maileingang.

Leider ist ein Fehler beim E-Mail-Versand aufgetreten. Bitte versuchen Sie es erneut.

Vorgang fortführen?

Exportieren
Filter
  • K+-ATPase  (1)
  • isothermal microcalorimetry  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Journal of thermal analysis and calorimetry 48 (1997), S. 437-445 
    ISSN: 1572-8943
    Schlagwort(e): cardiac glycoside binding ; DSC ; membrane protein ; membrane receptor binding ; Na+ ; K+-ATPase ; thermal unfolding ; titration calorimetry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract DSC studies are carried out to characterize Na+,K+-ATPase isolated from pig kidney in its natural membrane environment as well as in its purified state upon detergent treatment. The transition temperatures of the investigated thermal protein unfolding process, observed between 43 and 64.5° C, depend on the local membrane environment as well as onpH. In addition, the transition temperature is significantly increased upon binding of different cations and ligands which are known to interact with the enzyme. Evidence for a lipid phase transition around 23 °C in the original biological membrane is reported. The application of a calorimeter equipped with removable cells appears to be more suitable for the investigation of this type of membrane sample than an instrument with fixed capillary cells. Filling the sample capillary cell with an usual syringe, consisting of a long and thin needle, can influence the experimental results. Na+, K+ -ATPase acts as the receptor for cardiac glycoside binding. The thermodynamic parameters of this binding process are determined by titration calorimetry. The binding of ouabain, as a typical example, is unusually slow and is enthalpy driven. The enthalpy change upon binding enthalpy is −75 kJ mol−1 at 25 °C. The surprisingly low stoichiometric coefficient, resulting from an evaluation based on a simple one step binding model, is interpreted in terms of a dimeric receptor unit.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of thermal analysis and calorimetry 57 (1999), S. 75-85 
    ISSN: 1572-8943
    Schlagwort(e): amorphous phases ; Desferal ; gravimetry ; isothermal microcalorimetry ; recrystallization ; relative humidity ; water vapor sorption
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract The amorphous content of different Desferal samples was quantified by recording its recrystallization using isothermal microcalorimetry in a static as well as in a flowing humid atmosphere. Furthermore water vapor sorption gravimetry was performed for the same purpose. These analytical methods result in a quantitative signal directly dependent on the content of the amorphous phase (recrystallization, water sorption equilibrium). Their sensitivity allows the detection of amorphous content below 1%. Methods are compared and advantages and disadvantages are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
Schließen ⊗
Diese Webseite nutzt Cookies und das Analyse-Tool Matomo. Weitere Informationen finden Sie hier...