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  • 1
    Digitale Medien
    Digitale Medien
    Analysis of antigenic reactivity of synthetic monosaccharide lipid A analogues with monoclonal antibodies (1987)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 44 (1987), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: In vitro antigenic reactivity of chemically synthesized monosaccharide analogues of nonreducing sugar moiety of lipid A with 4 monoclonal antibodies against Salmonella minnesota R595 lipid A was studied by the inhibition test of enzyme-linked immunosorbent assay (ELISA). In the assays with 2 monoclonal antibodies (mAb 5G and mAb36G), which have been suggested to recognize the Salmonella type lipid A structure, no antigenic reactivity of the tested analogues was observed. However, several monosaccharide analogues exhibited antigenic reactivities with other monoclonal antibodies (mAb161M and mAb19M), especially some structural differences of hydrophobic and/or hydrophilic parts among monosaccharide analogues were recognized by mAb161M.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1987.tb02273.x
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  • 2
    Digitale Medien
    Digitale Medien
    Adenosine kinase-deficient mutant of Saccharomyces cerevisiae (1995)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 127 (1995), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract A cordycepin-resistant mutant strain of Saccharomyces cerevisiae (CD-R2) was found to be deficient in adenosine kinase. This mutant accumulated S-adenosylhomocysteine during growth in the presence of exogenous adenosine and it grew in a pseudohyphal manner in the presence of this nucleotide.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1995.tb07444.x
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  • 3
    Digitale Medien
    Digitale Medien
    Molecular cloning and characterization of the genes encoding the immunoreactive major cell-surface proteins of Porphyromonas gingivalis (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 120 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract A 72-kDa major cell-surface protein (72K-CSP) was purified from the wash fluid of Porphyromonas gingivalis OMZ409. Using the synthetic oligonucleotide probes corresponding to the determined amino-terminal amino acid sequence of 72K-CSP, recombinant plasmid clones carrying approx. 3.4-kb KpnI-XhoI fragments in XL1-Blue libraries of P. gingivalis OMZ409 and 381 were obtained. The premature form proteins of 558 and 563 amino acids led by putative signal sequences were thought to be processed to form the mature proteins of a predicted size of 55 655 Da for strain OMZ409 and of 55 654 for strain 381. Both proteins had unusual proline-rich regions in their carboxyl-terminal regions. No homologous sequences could be found in protein databases. Examination of antigen-specific antibody responses in the serum of patients with adult periodontitis by ELISA revealed that 72K-CSP had a different immunoreactivity from that of P. gingivalis 381 fimbriae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb07002.x
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  • 4
    Digitale Medien
    Digitale Medien
    Transformation as a tool for genetic fine mapping of closely located mutations in Thermus thermophilus (1994)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 117 (1994), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract Recombination indexes were measured among six Trp− mutants of Thermus thermophilus HB27 by reciprocal transformation among the mutants. Based on the index values, the order and distance of four closely located mutation sites were predicted. Cloning and sequence analysis of the mutants revealed that the order and distance among the mutation sites predicted from the index values were correct. A similar relationship between the index value and real distance was also obtained for two Pro− mutants. These results suggest that transformation can be used as a tool for genetic fine mapping in T. thermophilus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1994.tb06761.x
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  • 5
    Digitale Medien
    Digitale Medien
    Hemolytic toxin produced by Porphyromonas gingivalis (1993)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 114 (1993), S. 0 
    Details
    ISSN: 1574-6968
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: Abstract Porphyromonas gingivalis no. 381 and ATCC 33277 produced an extracellular hemolytic toxin which was heat-labile, trypsin-sensitive, and lytic to human, horse, sheep and rabbit erythrocytes. The hemolytic toxin is a ‘hot-cold’, thiol-independent toxin. The production of the hemolytic toxin was greatly enhanced by addition of hemoglobin to the culture medium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1574-6968.1993.tb06585.x
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  • 6
    Digitale Medien
    Digitale Medien
    Limonoid Debittering of Citrus Juice Sera By Immobilized Cells of Corynebacterium fascians (1985)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 50 (1985), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Corynebacterium fascians cells capable of metabolizing limonoids were prepared conveniently with inexpensive carbon sources such as fructose, galactose and citric acid. Cells thus obtained were immo-bilized in acrylamide gel and used in a biological debittering process previously developed. The process significantly reduced limonin and nomilin contents of citrus juice sera. It was particularly effective toward the reduction of nomilin. The debittering treatment did not have adverse effects on the composition of other citrus constituents such as citric, malic, ascorbic acids, fructose, glucose and sucrose.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.1985.tb13393.x
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  • 7
    Digitale Medien
    Digitale Medien
    A New Pectic Acid Transeliminase Produced Exocellularly by a Bacillus (1966)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 31 (1966), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A new type of endo-pectic acid transeliminase was isolated from the culture medium of a strain of Bacillus. The enzyme attacked pectic substances randomly and produced un saturated trigalacturonic acid as the major end product. The optimum pH on either acid-soluble pectic acid or tetragalaeturonic acid was 9.3–9.7, and the enzyme required calcium ions for maximum activity. Strontium was the only other divalent cation which stimulated activity. Trigalacturonic acid was attacked very slowly. The major site of attack of tetragalacturonic acid was the glycosidic bond on the nonreducing end. Unsaturated tetragalacturonic acid was also attacked at the central bond. The cleavage of pentagalacturouic acid occurred preferentially at bond 3, followed by 4 and 2, in order. The glycosidic bond on the reducing end is called bond 1. With unsaturated pentagalacturonic acid, it was concluded that the major site of cleavage was at bond 3, with a much slower rate at bond 2.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.1966.tb03259.x
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  • 8
    Digitale Medien
    Digitale Medien
    SHRINKAGE IN DEHYDRATION OF ROOT VEGETABLES (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The shrinkage in dehydration of root vegetables such as carrots, potatoes, sweet potatoes and radishes was investigated. The samples were dried in flowing hot air. The surface areas were measured by photographing the samples. Three drying models were postulated for the formulation of the relation between the changes of the surface area and the moisture contents. The uniform drying model, which in the case of drying is that the shrinkage in volume equals the volume of water lost by the evaporation, agreed with the measured values during the early stages of the drying. The core drying model by assuming the formation of the dried layer at outer side of material was better in agreement with the experimental results. The semicore drying model, which is the intermediate model between the uniform and the core drying model, and the empirical equations of the shrinkage were also considered.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.1976.tb14414.x
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  • 9
    Digitale Medien
    Digitale Medien
    PRODUCTION OF LIMONOATE DEHYDROGENASE BY Pseudomonas (1976)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 41 (1976), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The growth conditions of Pseudomonas sp. 321–18 were studied to maximize yields of limonoate dehydrogenase, which prevents the development of limonin bitterness in citrus juices. The organism grew well on 0.5% or less limonoate media, but it did not grow on media containing 1.0% or more limonoate. Galactose and fructose were excellent substrates for its growth, but sucrose and glucose were found to be poor. The organism grew best at 25°C and pH 7–7.5. Cells grown on substrates other than those containing limonoids did not produce limonoate dehydrogenase, but the enzyme was induced with low concentrations of limonoids. Enzyme activity was increased five to sixfold by use of the induction method on galactose grown cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1095-8649.2007.volcontents_1.x-i1
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  • 10
    Digitale Medien
    Digitale Medien
    EFFECT OF MICROBIAL GROWTH UPON MYOFIBRILLAR PROTEINS (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 35 (1970), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: SUMMARY— Aseptic samples from pig and rabbit muscles were inoculated with Achromobacter liquefaciens, Micrococcus luteus, Pediococcus cerevisiae, Pseudomonas fluorescens, Streptococcus faecal is end a mixed culture obtained from commercial hamburger. Some difficulty was encountered in getting the organisms to grow, and good growth was achieved only with A. liquefaciens end mixed culture from commercial meat. Both inoculated and uninoculated control samples were incubated at 3 and 10°C for 0, 8 and 20 days. The salt soluble proteins were extracted with Weber-Edsall solution and subjected to sucrose density gradient centrifugation, gel filtration and disc gel electrophoresis. The microorganisms utilized in this study had no measurable effect upon the myofibrillar proteins from either pig or rabbit muscle. However, bacterial growth decreased the amount of certain non-protein ultra-violet absorbing components in the ultracentrifugal pattern of Weber-Edsall extract. These components did not appear to be of myofibrillar origin. Disc gel patterns of Weber-Edsall extracts from pig muscle produced a more intensely staining band than those from rabbit muscle at Rm, = 0.59.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.1970.tb00971.x
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