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  • 1
    Publication Date: 2020-06-24
    Description: The synthetic production of monodisperse single magnetic domain nanoparticles at ambient temperature is challenging. In nature, magnetosomes--membrane-bound magnetic nanocrystals with unprecedented magnetic properties--can be biomineralized by magnetotactic bacteria. However, these microbes are difficult to handle. Expression of the underlying biosynthetic pathway from these fastidious microorganisms within other organisms could therefore greatly expand their nanotechnological and biomedical applications. So far, this has been hindered by the structural and genetic complexity of the magnetosome organelle and insufficient knowledge of the biosynthetic functions involved. Here, we show that the ability to biomineralize highly ordered magnetic nanostructures can be transferred to a foreign recipient. Expression of a minimal set of genes from the magnetotactic bacterium Magnetospirillum gryphiswaldense resulted in magnetosome biosynthesis within the photosynthetic model organism Rhodospirillum rubrum. Our findings will enable the sustainable production of tailored magnetic nanostructures in biotechnologically relevant hosts and represent a step towards the endogenous magnetization of various organisms by synthetic biology.
    Type: Article , PeerReviewed
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  • 2
    Publication Date: 2017-06-09
    Description: The new aromatic polyketides genoketide A1, genoketide A2 and prechrysophanol glucuronide are biosynthetic intermediates of the octaketide chrysophanol. They were isolated from the alkaliphilic strain Streptomyces sp. AK 671 together with the new metabolite chrysophanol glucuronide. The structures of the compounds were elucidated by mass spectrometry and NMR methods. Genoketide A2 exhibited a slight and prechrysophanol glucuronide a more pronounced inhibition of the proliferation of L5178y lymphoma cells.
    Type: Article , PeerReviewed
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  • 3
    Publication Date: 2016-11-03
    Description: Cold-water corals (CWC) are widely distributed around the world forming extensive reefs at par with tropical coral reefs. They are hotspots of biodiversity and organic matter processing in the world’s deep oceans. Living in the dark they lack photosynthetic symbionts and are therefore considered to depend entirely on the limited flux of organic resources from the surface ocean. While symbiotic relations in tropical corals are known to be key to their survival in oligotrophic conditions, the full metabolic capacity of CWC has yet to be revealed. Here we report isotope tracer evidence for efficient nitrogen recycling, including nitrogen assimilation, regeneration, nitrification and denitrification. Moreover, we also discovered chemoautotrophy and nitrogen fixation in CWC and transfer of fixed nitrogen and inorganic carbon into bulk coral tissue and tissue compounds (fatty acids and amino acids). This unrecognized yet versatile metabolic machinery of CWC conserves precious limiting resources and provides access to new nitrogen and organic carbon resources that may be essential for CWC to survive in the resource-depleted dark ocean.
    Type: Article , PeerReviewed , info:eu-repo/semantics/article
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  • 4
    Publication Date: 2019-09-23
    Description: Ocean islands, seamounts and volcanic ridges are thought to form above mantle plumes. Yet, this mechanism cannot explain many volcanic features on the Pacific Ocean floor and some might instead be caused by cracks in the oceanic crust linked to the reorganization of plate motions. A distinctive bend in the Hawaiian–Emperor volcanic chain has been linked to changes in the direction of motion of the Pacific Plate, movement of the Hawaiian plume, or a combination of both. However, these links are uncertain because there is no independent record that precisely dates tectonic events that affected the Pacific Plate. Here we analyse the geochemical characteristics of lava samples collected from the Musicians Ridges, lines of volcanic seamounts formed close to the Hawaiian–Emperor bend. We find that the geochemical signature of these lavas is unlike typical ocean island basalts and instead resembles mid-ocean ridge basalts. We infer that the seamounts are unrelated to mantle plume activity and instead formed in an extensional setting, due to deformation of the Pacific Plate. 40Ar/39Ar dating reveals that the Musicians Ridges formed during two time windows that bracket the time of formation of the Hawaiian–Emperor bend, 53–52 and 48–47 million years ago. We conclude that the Hawaiian–Emperor bend was formed by plate–mantle reorganization, potentially triggered by a series of subduction events at the Pacific Plate margins.
    Type: Article , PeerReviewed
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  • 5
    Publication Date: 2020-10-26
    Description: The origin of the Christmas Island Seamount Province in the northeast Indian Ocean is enigmatic. The seamounts do not form the narrow, linear and continuous trail of volcanoes that would be expected if they had formed above a mantle plume1, 2. Volcanism above a fracture in the lithosphere3 is also unlikely, because the fractures trend orthogonally with respect to the east–west trend of the Christmas Island chain. Here we combine 40Ar/39Ar age, Sr, Nd, Hf and high-precision Pb isotope analyses of volcanic rocks from the province with plate tectonic reconstructions. We find that the seamounts are 47–136 million years old, decrease in age from east to west and are consistently 0–25 million years younger than the underlying oceanic crust, consistent with formation near a mid-ocean ridge. The seamounts also exhibit an enriched geochemical signal, indicating that recycled continental lithosphere was present in their source. Plate tectonic reconstructions show that the seamount province formed at the position where West Burma began separating from Australia and India, forming a new mid-ocean ridge. We propose that the seamounts formed through shallow recycling of delaminated continental lithosphere entrained in mantle that was passively upwelling beneath the mid-ocean ridge. We conclude that shallow recycling of continental lithosphere at mid-ocean ridges could be an important mechanism for the formation of seamount provinces in young ocean basins.
    Type: Article , PeerReviewed
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  • 6
    Publication Date: 2019-01-21
    Description: Circumpolar surface waters dominate the circulation of the Southern Ocean and sustain one of the ocean's largest standing stocks of biomass thereby producing a significant output of biogenic components, mainly diatoms, to the bottom sediments. Generally transit of biogenic matter from the sea surface to the sea floor affects nutrient regeneration fuels benthic life and transfers signals to the sediment record1–5. Reliable quantification of the relationship between biological production, fractionation of skeletal and tissue components and bottom sediment accumulation depends on direct vertical flux measurements from sediment trap deployments6–9, which have proved to be most scientifically productive10–13. We now present data on vertical mass fluxes from the Southern Ocean and evidence for strong biogeochemical fractionation between organic carbon-, nitrogen- and phosphorus-containing compounds, siliceous and calcareous skeletal remains, and refractory aluminosilicates.
    Type: Article , PeerReviewed
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  • 7
    Publication Date: 2019-03-05
    Description: Coccolithophores have influenced the global climate for over 200 million years. These marine phytoplankton can account for 20 per cent of total carbon fixation in some systems. They form blooms that can occupy hundreds of thousands of square kilometres and are distinguished by their elegantly sculpted calcium carbonate exoskeletons (coccoliths), rendering them visible from space. Although coccolithophores export carbon in the form of organic matter and calcite to the sea floor, they also release CO 2 in the calcification process. Hence, they have a complex influence on the carbon cycle, driving either CO 2 production or uptake, sequestration and export to the deep ocean. Here we report the first haptophyte reference genome, from the coccolithophore Emiliania huxleyi strain CCMP1516, and sequences from 13 additional isolates. Our analyses reveal a pan genome (core genes plus genes distributed variably between strains) probably supported by an atypical complement of repetitive sequence in the genome. Comparisons across strains demonstrate that E. huxleyi, which has long been considered a single species, harbours extensive genome variability reflected in different metabolic repertoires. Genome variability within this species complex seems to underpin its capacity both to thrive in habitats ranging from the equator to the subarctic and to form large-scale episodic blooms under a wide variety of environmental conditions. © 2013 Macmillan Publishers Limited. All rights reserved.
    Type: Article , PeerReviewed
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