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  • Articles  (17)
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  • 1
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    Genes, Vol. 9, Pages 639: De Novo Analysis Reveals Transcriptomic Responses in Eriobotrya japonica Fruits during Postharvest Cold Storage (2018)
    MDPI
    In: Genes
    Details
    Publication Date: 2018
    Description: Cold storage is the primary preservation method of postharvest loquat fruits. However, cold storage also results in many chilling injury physiological disorders called lignification, which decreases the quality and economic value of the fruits. Few studies to date have focused on the transcriptomic responses associated with lignification except lignin synthesis pathways. This study aimed to explore the changes of loquat transcriptome during long-term cold storage. Our results showed that the gene expression patterns were differed among the five stages. The differentially expressed genes (DEGs) in response to cold storage were more intense and complex in earlier stage. The membrane-related genes preferentially responded to low temperature and were followed by intracellular-located genes. The cold-induced pathways were mainly concerned with signal transduction and secondary metabolism (i.e., lignin, pectin, cellulose, terpenoid, carotenoid, steroid) in the first three stages and were chiefly related to primary metabolism in the later two stages, especially energy metabolism. Further investigation suggested that 503 protein kinases, 106 protein phosphatases, and 40 Ca2+ signal components were involved in the cold signal transduction of postharvest loquat fruits. We predicted a pathway including 649 encoding genes of 49 enzymes, which displayed the metabolisms of major sugars and polysaccharides in cold-stored loquat fruits. The coordinated expression patterns of these genes might contribute to the changes of saccharides in the pathway. These results provide new insight into the transcriptomic changes of postharvest loquat fruits in response to cold storage environment, which may be helpful for improving the postharvest life of loquat in the future.
    Electronic ISSN: 2073-4425
    Topics: Biology
    Published by MDPI
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  • 2
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    Alkaloids from the Stems of Glycosmis pentaphylla (2012)
    Wiley
    Details
    Publication Date: 2012-08-18
    Description: A new simple carbazole alkaloid, 4-(7-hydroxy-3-methoxy-6-methyl-9 H -carbazol-4-yl)but-3-en-2-one ( 1 ), and two new dimeric carbazole alkaloids, bisglybomine B ( 2 ) and biscarbalexine A ( 3 ), together with seven known alkaloids, were isolated from the stems of Glycosmis pentaphylla. Their structures were elucidated by spectroscopic methods, especially 2D-NMR techniques.
    Print ISSN: 0018-019X
    Electronic ISSN: 1522-2675
    Topics: Chemistry and Pharmacology
    Published by Wiley
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  • 3
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    A novel high-yield synthesis of aminoacyl p-nitroanilines and aminoacyl 7-amino-4-methylcoumarins: Important synthons for the synthesis of chromogenic/fluorogenic protease substrates (2011)
    Beilstein-Institut
    Details
    Publication Date: 2011-07-28
    Description: Aminoacyl p -nitroaniline (aminoacyl- p NA) and aminoacyl 7-amino-4-methylcoumarin (aminoacyl-AMC) are important synthons for the synthesis of chromogenic/fluorogenic protease substrates. A new efficient method was developed to synthesize aminoacyl- p NA and aminoacyl-AMC derivatives in excellent yields starting from either amino acids or their corresponding commercially available N -hydroxysuccinimide esters. The method involved the in situ formation of selenocarboxylate intermediate of protected amino acids and the subsequent non-nucleophilic amidation with an azide. Common protecting groups used in amino acid/peptide chemistry were all well-tolerated. The method was also successfully applied to the synthesis of a dipeptide conjugate, indicating that the methodology is applicable to the synthesis of chromogenic substrates containing short peptides. The method has general applicability to the synthesis of chromogenic and fluorogenic peptide substrates and represents a convenient and high-yield synthesis of N α -protected-aminoacyl- p NAs/AMCs, providing easy access to these important synthons for the construction of chromogenic/fluorogenic protease substrates through fragment condensation or stepwise elongation. Beilstein J. Org. Chem. 2011, 7, 1030–1035. doi:10.3762/bjoc.7.117
    Keywords: 7-amino-4-methylcoumarinp-nitroanilineproteolytic substrateselenocarboxylate/azide amidationsynthon
    Electronic ISSN: 1860-5397
    Topics: Chemistry and Pharmacology
    Published by Beilstein-Institut
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  • 4
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    Energies, Vol. 12, Pages 1239: An Adaptive Hybrid Learning Paradigm Integrating CEEMD, ARIMA and SBL for Crude Oil Price Forecasting (2019)
    MDPI
    In: Energies
    Details
    Publication Date: 2019
    Description: Crude oil is one of the main energy sources and its prices have gained increasing attention due to its important role in the world economy. Accurate prediction of crude oil prices is an important issue not only for ordinary investors, but also for the whole society. To achieve the accurate prediction of nonstationary and nonlinear crude oil price time series, an adaptive hybrid ensemble learning paradigm integrating complementary ensemble empirical mode decomposition (CEEMD), autoregressive integrated moving average (ARIMA) and sparse Bayesian learning (SBL), namely CEEMD-ARIMA&SBL-SBL (CEEMD-A&S-SBL), is developed in this study. Firstly, the decomposition method CEEMD, which can reduce the end effects and mode mixing, was employed to decompose the original crude oil price time series into intrinsic mode functions (IMFs) and one residue. Then, ARIMA and SBL with combined kernels were applied to predict target values for the residue and each single IMF independently. Finally, the predicted values of the above two models for each component were adaptively selected based on the training precision, and then aggregated as the final forecasting results using SBL without kernel-tricks. Experiments were conducted on the crude oil spot prices of the West Texas Intermediate (WTI) and Brent crude oil to evaluate the performance of the proposed CEEMD-A&S-SBL. The experimental results demonstrated that, compared with some state-of-the-art prediction models, CEEMD-A&S-SBL can significantly improve the prediction accuracy of crude oil prices in terms of the root mean squared error (RMSE), the mean absolute percent error (MAPE), and the directional statistic (Dstat).
    Electronic ISSN: 1996-1073
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by MDPI
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  • 5
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    Sensors, Vol. 17, Pages 1348: Rapid Waterborne Pathogen Detection with Mobile Electronics (2017)
    MDPI Publishing
    In: Sensors
    Details
    Publication Date: 2017-06-10
    Description: Pathogen detection in water samples, without complex and time consuming procedures such as fluorescent-labeling or culture-based incubation, is essential to public safety. We propose an immunoagglutination-based protocol together with the microfluidic device to quantify pathogen levels directly from water samples. Utilizing ubiquitous complementary metal–oxide–semiconductor (CMOS) imagers from mobile electronics, a low-cost and one-step reaction detection protocol is developed to enable field detection for waterborne pathogens. 10 mL of pathogen-containing water samples was processed using the developed protocol including filtration enrichment, immune-reaction detection and imaging processing. The limit of detection of 10 E. coli O157:H7 cells/10 mL has been demonstrated within 10 min of turnaround time. The protocol can readily be integrated into a mobile electronics such as smartphones for rapid and reproducible field detection of waterborne pathogens.
    Electronic ISSN: 1424-8220
    Topics: Chemistry and Pharmacology , Electrical Engineering, Measurement and Control Technology
    Published by MDPI Publishing
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  • 6
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    Association between serum free fatty acid levels and nonalcoholic fatty liver disease: a cross-sectional study (2014)
    Springer Nature
    Details
    Publication Date: 2014-07-26
    Description: High serum free fatty acid (FFA) levels are associated with metabolic syndrome (MS). This study aimed to assess the association of fasting serum FFAs with nonalcoholic fatty liver disease (NAFLD) in a Chinese population. A total of 840 subjects fulfilled the diagnostic criteria of NAFLD and 331 healthy control participants were enrolled in this cross-sectional study. Fasting serum FFA levels and other clinical and laboratory parameters were measured. NAFLD patients had significantly higher serum FFA levels than controls (P 〈 0.001). Serum FFA levels were significantly and positively correlated with parameters of MS, inflammation indexes, and markers of hepatocellular damage. Elevated serum FFA levels were found in NAFLD subjects with individual components of MS (obesity, hypertriglyceridaemia, and hyperglycaemia). Stepwise regression showed that serum FFA levels were an independent factor predicting advanced fibrosis (FIB-4 ≥ 1.3) in NAFLD patients. Serum FFA levels correlated with NAFLD and could be used as an indicator for predicting advanced fibrosis in NAFLD patients. Scientific Reports 4 doi: 10.1038/srep05832
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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  • 7
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    The significance of serum uric acid level in humans with acute paraquat poisoning (2015)
    Springer Nature
    Details
    Publication Date: 2015-03-17
    Description: Hyperuricemia is a strong and independent predictor of all-cause mortality in cardiovascular disease and has been found to play a role in diseases exacerbated by oxidative stress and inflammation. This study aimed to evaluate whether serum uric acid (UA) level is an indicator of outcome in patients with acute paraquat poisoning. A total of 205 subjects who had attempted suicide by oral ingestion of paraquat were admitted to the emergency room between January 2009 and June 2014. Initial serum UA level and other laboratory parameters were measured. A total of 66 patients died during the 30 days after admission, corresponding to a 32.2% cumulative incidence of mortality. UA levels were higher in non-survivors than survivors (P 〈 0.001) and 30-day mortality increased with increasing baseline serum UA level (P 〈 0.001). In a prediction analysis for 30-day mortality, the serum UA level had a cut-off concentration of 284 µmol/L in female patients and 352 µmol/L in male patients. Multivariate Cox proportional hazards regression analyses showed that white blood cell counts and UA were independent prognostic factors. In conclusion, we showed that serum UA may be an independent predictor of 30-day mortality in patients with paraquat poisoning. Scientific Reports 5 doi: 10.1038/srep09168
    Electronic ISSN: 2045-2322
    Topics: Natural Sciences in General
    Published by Springer Nature
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  • 8
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    3D Printing of Amino Resin-based Photosensitive Materials on Multi-parameter Optimization Design for Vascular Engineering Applications (2019)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2019-08-24
    Description: Cardiovascular diseases are currently the most common cause of death globally and of which, the golden treatment method for severe cardiovascular diseases or coronary artery diseases are implantations of synthetic vascular grafts. However, such grafts often come with rejections and hypersensitivity reactions. With the emergence of regenerative medicine, researchers are now trying to explore alternative ways to produce grafts that are less likely to induce immunological reactions in patients. The main goal of such studies is to produce biocompatible artificial vascular grafts with the capability of allowing cellular adhesion and cellular proliferation for tissues regeneration. The Design of Experimental concepts is employed into the manufacturing process of digital light processing (DLP) 3D printing technology to explore near-optimal processing parameters to produce artificial vascular grafts with vascular characteristics that are close to native vessels by assessing for the cause and effect relationships between different ratios of amino resin (AR), 2-hydroxyethyl methacrylate (HEMA), dopamine, and curing durations. We found that with proper optimization of fabrication procedures and ratios of materials, we are able to successfully fabricate vascular grafts with good printing resolutions. These had similar physical properties to native vessels and were able to support cellular adhesion and proliferation. This study could support future studies in exploring near-optimal processes for fabrication of artificial vascular grafts that could be adapted into clinical applications.
    Electronic ISSN: 2073-4360
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Process Engineering, Biotechnology, Nutrition Technology
    Published by Molecular Diversity Preservation International
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  • 9
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    An Idealized Landslide Failure Surface and Its Impacts on the Traveling Paths (2020)
    Frontiers Media
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    Publication Date: 2020-08-21
    Electronic ISSN: 2296-6463
    Topics: Geosciences
    Published by Frontiers Media
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  • 10
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    Predicting the HMA-LMA Status in Marine Sponges by Machine Learning (2017)
    Frontiers Media
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    Publication Date: 2017-05-08
    Electronic ISSN: 1664-302X
    Topics: Biology
    Published by Frontiers Media
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