ISSN:
0021-9304
Keywords:
ceramics
;
calcium phosphates
;
surface reactions
;
surface transformations
;
in vitro
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
,
Technology
Notes:
Changes occurring at the surface of a calcium phosphate coating when in contact with osteoblasts versus those in acellular solutions were analyzed. The coating studied is one with a well-documented extensive effect on short-term bone growth stimulation. Precipitates associated with original crystals and organized in a weblike structure were observed after a 3-week culture with osteoblasts. The precipitates were identified as carbonated hydroxyapatite (c-HA). In contrast, no significant surface changes were detected after immersion in an acellular serum-containing solution. However, in an acellular serum-free solution simulating the ionic composition of plasma, precipitates, identified as c-HA, were abundantly formed. Dissolution of the original coating preceded precipitation. The data support the hypothesis that dissolution of synthetic calcium phosphate ceramics is an initial step in their transformation to a biologically equivalent apatite, and suggest that both solution-mediated (dissolution-precipitation) and cell-mediated mechanisms are involved in the surface transformation. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 234-243, 1998.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
Permalink
