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Functional expression of Aquaspirillum magnetotacticum genes in Escherichia coli K12 (1988)

Waleh, Nahid S.

Springer

Molecular genetics and genomics 214 (1988), S. 592-594

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ISSN: 1617-4623

Keywords: Gene library ; Recombinant cosmid ; Aquaspirillum magnetotacticum ; magnetotaxis

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Gene libraries from the magnetotactic bacterium, Aquaspirillum magnetotacticum were constructed in Escherichia coli with cosmids pLAFR3 and c2RB as vectors. Recombinant cosmids able to complement the thr-1, leuB, and proA mutations of the host were identified. The Pro+ recombinant cosmid restored wild-type phenotype in proA and proB but not in the proC mutants of E. coli. The results of restriction endonuclease digestion and Southern hybridization analysis indicate that the relevent leu and pro biosynthetic genes of A. magnetotacticum are not closely linked on the chromosome.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00330500

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