ISSN:
1573-5044
Keywords:
antibiotic
;
kanamycin
;
Linum usitatissimum
;
selection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Flax (Linum usitatissimum L.) hypocotyls were cultivated on regeneration media containing various concentrations of kanamycin (an aminoglycoside antibiotic commonly used to select transgenic plant material) solidified with three different gelling agents: gellan gum, agar and a mixture of both. The inhibitory effect of kanamycin on bud regeneration was analyzed. A significant interaction was observed between the nature of the gelling agent and the kanamycin concentration. The antibiotic concentration needed to strongly inhibit bud production varied greatly with the nature of the gelling agent. Gellan gum lowered the inhibitory effect of kanamycin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1006455918041
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