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  • 1
    Electronic Resource
    Electronic Resource
    Cytological changes in the pars distalis of the female rat hypophysis grafted under the kidney capsule (1979)
    Springer
    Cell & tissue research 199 (1979), S. 539-543 
    Details
    ISSN: 1432-0878
    Keywords: Pars distalis ; Graft ; Ultrastructure ; Prolactin ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Intact female rats received transplants of two hypophysial partes distales under the kidney capsule. The plasma levels of prolactin were determined, and the ultrastructure of the grafted gland was studied 15, 45 and 90 days after the operation. Although prolactin levels in the three experimental groups were significantly higher than those in control rats, a decrease in prolactin level was detected in the 45-day samples. Parallel ultrastructural changes suggest that between the 45th and 90th postoperative day a process is initiated leading to hyperplasia and hypertrophy of prolactotrophs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236089
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  • 2
    Electronic Resource
    Electronic Resource
    Herring bodies; an electron microscopic study of local degeneration and regeneration of neurosecretory axons (1970)
    Springer
    Cell & tissue research 111 (1970), S. 293-315 
    Details
    ISSN: 1432-0878
    Keywords: Herring bodies ; Ultrastructure ; Degeneration ; Regeneration ; Neurosecretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Herring bodies in the posterior lobe of the bovine hypophysis are very large (2–600 μ) and can be classified into three types. The type I Herring body contains an accumulation of neurosecretory granules. These Herring bodies are very scarce and should not be confused with the numerous, but small, axonal swellings which also contain neurosecretory granules. The type II Herring body is characterized by the presence of a varying number of normal, moderately electron dense and “empty” vesicles, autophagic vacuoles, multilamellate bodies and occasional mitochondria. These Herring bodies are frequently observed. The type III Herring body is typified by the presence of dense vesicles connected to tubular formations which contain material of variable electron density, of filaments, and of long slender and very numerous mitochondria. The presence of multilamellate bodies and autophagic vacuoles suggests that the type II Herring body is in a degenerating phase. This concept is further substantiated by the similarity between this type of Herring body and transected neurosecretory axons in which degeneration is occurring. A similar comparison suggests that the type III Herring body is undergoing a regenerative process. Our current concept of the structure and function of Herring bodies is revised in the discussion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00342485
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  • 3
    Electronic Resource
    Electronic Resource
    Ultrastructure of the rat pineal gland grafted under the kidney capsule (1977)
    Springer
    Cell & tissue research 176 (1977), S. 131-142 
    Details
    ISSN: 1432-0878
    Keywords: Pineal gland ; Graft ; Ultrastructure ; Innervation ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pineal glands were grafted under the kidney capsule of mature male rats for periods of 20, 40, 60 and 100 days. Each grafted gland was then excised and divided into two halves. One half was processed for conventional electron microscopy and the other was fixed in aldehydes and then incubated in a zinc iodide-osmium tetroxide mixture at pH 4.4 (A-ZIO-4.4). During the forty days following the operation pinealocytes showed the typical ultrastructural features associated with cells with a high protein and/or peptide secretory activity. On the other hand, during this period, the number of granular vesicles decreased progressively. From day 40 on, the grafted pinealocytes lacked granular vesicles. During the second half of the experimental period the ultrastructure of the pinealocytes indicated that their secretory activity was considerably decreased. During the acute phase of the experimental period numerous structures regarded as the tip of growing axons as well as typical nerve fibres appeared around blood vessels and within the parenchyma of the grafted gland. In the transplanted tissue obtained 60 and 100 days after the operation the growth cones were scarce, whereas typical nerve endings became numerous. These endings contained small clear vesicles which reacted positively when the tissue was treated with A-ZIO-4.4. The secretory activity of the grafted pineal gland and the nature of the nerve fibres which innervate the graft are discussed. The authors wish to thank Mrs. E.M. Rodríguez de Calderón for her valuable help
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220349
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  • 4
    Electronic Resource
    Electronic Resource
    Immunocytochemistry and ultrastructure of the neuropil located ventral to the rat supraoptic nucleus (1984)
    Springer
    Cell & tissue research 236 (1984), S. 171-180 
    Details
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Ultrastructure ; Supraoptic nucleus ; Neuropil ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The neuropil located ventral to the SON was investigated by the use of immunoperoxidase staining for neurophysins, oxytocin and vasopressin, and electron miroscopy. The study was performed in six groups of rats: 1) control; 2) infusion of isotonic saline into the CSF; 3) infusion of hypertonic saline into the CSF; 4) drinking hypertonic saline for 4 days; 5) same as group 4 but injection of colchicine into the CSF on second day of dehydration; 6) salt loading for 3 months. In the control rats the ventral neuropil contained a few immunoreactive processes, the general morphology of which was completely different from that of the neurosecretory axons emerging from the SON at its dorsal aspect. In rats of groups 3 to 6 the ventral processes (VP) became loaded with neurosecretory granules, whereas the perikarya and axons were depleted. Based on their general morphology and reactivity pattern it is suggested that the VP are dendrites. Most of these “dendrites” were embedded in a glial cushion formed by the processes of a particular type of marginal glia. Some of these “dendrites” enveloped an arteriole penetrating the optic tract. All VP were rich in synaptic contacts. The possibility that the VP of neurosecretory cells may be functionally related to the subarachnoid CSF and the arteriolar blood flow is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216528
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  • 5
    Electronic Resource
    Electronic Resource
    Control of the pars intermedia of the lizard, Anolis carolinensis (1978)
    Springer
    Cell & tissue research 186 (1978), S. 241-258 
    Details
    ISSN: 1432-0878
    Keywords: Neural lobe ; Anolis carolinensis ; Ultrastructure ; Intermediate lobe control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of the neural lobe of the lizard, Anolis carolinensis, was studied after fixation in a threefold aldehyde solution. The neural lobe appeared as narrow vertical diverticula separated from one another and from the pars intermedia by a continuous vascular septum. No nerves passed through this septum. The ependymal, fibrous and external layers were readily recognized. Peptidergic fibres were the main component of the fibrous layer. The peptidergic endings were in intimate contact with the ependymal cells, suggesting that the ependyma mediates the release of neural lobe peptides. The external layer contained ependymal end-feet and numerous aminergic terminals, ending directly on the perivascular basal lamina and/or on the ependymal end-feet. The functional aspects are discussed in terms of intermediate lobe control. The findings suggest that aminergic substances take part in the control of the intermedia, but do not exclude the involvement of peptide hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225534
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  • 6
    Electronic Resource
    Electronic Resource
    Ultrastructure and hormonal content of the proximal stump of the transected hypothalamo-hypophysial tract of the frog (Rana pipiens) (1970)
    Springer
    Cell & tissue research 104 (1970), S. 449-470 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamo-hypophysial tract ; Rana pipiens ; Transection-hormonal content ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several types of neurosecretory fibers were observed in the normal infundibulum of the frog. After transection of the median eminence, these neurosecretory fibers of the proximal stump reacted asynchronously, but followed approximately the same pattern: a “passive accumulation” of granules observed early after the transection was followed by an “active axonal reaction” with the appearance of numerous tubular formations which are thought to be related to the Golgi apparatus. They filled the axon almost completely, and then became dilated and filled with an electron dense material. Subsequently these dilatations pinched off and gave origin to new neurosecretory granules. These locally packed granules plus others which were probably formed in more proximal parts of the axon, and the perikaryon and then transported distally, accumulated in the proximal axonal stumps and started to fill the fibers retrogradely. There was a parallelism between the increase of tubular formations and neurosecretory granules larger then 1,500 Å in diameter, on one side, and the vasopressor activity of the proximal stump, on the other. The latter increased at an approximate rate of 1 mU/stump/day. The regeneration of the fibers of the hypothalamo-median eminence system is suggested by the presence in the proximal stump of fibers filled with granules smaller than 1,000 Å in diameter (normally seen in the median eminence) and the fact that 40% of the vasopressor activity of the extracts was not abolished by the thioglycollate treatment, which could be due to the presence of vasopressor amines other than adrenaline. The appearance towards the end of the observation period of a few “nerve endings” of several types contacting the perivascular basement membrane of vessels of the proximal stump would indicate that the neural lobe and median eminence functions were being reestablished, at least partially.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00335370
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