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  • Ultrastructure  (5)
  • ENERGY PRODUCTION AND CONVERSION  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 236 (1984), S. 693-697 
    ISSN: 1432-0878
    Keywords: Intermediate filaments ; Microtubules ; Caveolae ; Bullfrog ; Arachnoid mater ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the bullfrog, the meninges surrounding the central nervous system include an arachnoid mater that contains layers of cells with abundant intermediate filaments (IFs) having unique organizational characteristics. This membrane contains an inner lamina of cells that resemble fibroblasts and an outer lamina of flattened cells that are almost filled with IFs. The IFs of the outer arachnoid are arranged in compact, arching bundles that lie parallel to the outer surface of the central nervous system. Thus, sections cut tangentially to the membrane reveal bending of filament bundles, whereas transverse sections do not. In some cells bordering the subdural space, bundles of filaments are organized into highly-ordered spiral arrays. Attachments to the numerous desmosomes and, apparently, to the nuclear envelope suggest anchoring of cytoplasmic structures by the IF system. Microtubules occur primarily near the plasma membrane and the nucleus. Numerous caveolae also are associated with the plasma membrane. The unusual abundance, organization, and cytoplasmic relations of IFs in the bullfrog arachnoid suggest that this membrane may serve as an important model for study of fundamental cytoskeletal relations and function.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 202 (1979), S. 479-491 
    ISSN: 1432-0878
    Keywords: Ependyma ; Ultrastructure ; Posterior tela choroidea ; Rhombencephalon ; Rana catesbeiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The posterior rhombencephalic tela choroidea of the bullfrog was examined by electron microscopy. This membrane, the pia-ependymal roof of the caudal hindbrain, contains a large central region characterized by cuboidal ependymal cells which surround sizable microscopic apertures — the interependymal pores. Ultrastructurally ependymal cells of this area are characterized by infrequent apical microvilli and cilia. They contain irregularly shaped nuclei and few cytoplasmic organelles that are largely apical in position. The most striking feature is an abundance of cytoplasmic filaments forming an extensive cytoskeleton. Laterally these cells are joined by numerous elaborate desmosomes. The majority of the ependymal cells have a basal lamina consisting of single, double, or triple laminae lying parallel to the basal plasma membrane. Several unusual specializations are seen at the margins of the interependymal pores. The ependymal cells have lateral cytoplasmic processes that form the actual border of each pore. These processes originate from the apical surface of the cell and partially enclose an elaborate network of basal lamina associated with the interependymal pores. These findings demonstrate microscopic apertures in the roof of the fourth ventricle in the bullfrog that are associated with an unusual form of supportive ependyma.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 210 (1980), S. 121-132 
    ISSN: 1432-0878
    Keywords: Glycoproteins ; Ultrastructure ; Tunicamycin ; Colonic epithelium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Glycoproteins are associated with several structures of colonic absorptive cells of the mouse. These include the cell coat, Golgi apparatus and vesicles that transport the glycoproteins from the apparatus to the cell surface (Michaels and Leblond 1976). In many in vitro systems, the antibiotic tunicamycin inhibits the glycosylation of asparagine residues yielding carbohydrate-poor glycoproteins. In the present in vivo study, tunicamycin was injected into mice. The murine colonic epithelial cells were prepared routinely for electron microscopy and cytochemistry. Cells from the experimental and control animals were similar morphologically. However, staining by the periodic acid-chromic acid-silver methenamine technique, revealed differences in the distribution of glycoproteins. In animals that received the higher dosages of tunicamycin there was a substantial reduction in silver staining in both the Golgi apparatus and the vesicles of colonic epithelial cells compared to these structures in cells of identically treated control tissues, whereas the staining over the cell coat was not significantly altered. Possible explanations for the staining of the cell coat in the treated animals were provided in the text. This report demonstrates the feasibility of using tunicamycin in vivo and detection of the changes obtained by the silver methenamine method.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 228 (1983), S. 323-335 
    ISSN: 1432-0878
    Keywords: Ultrastructure ; Colonic epithelium ; Colchicine ; Vesicles ; Glycoproteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In murine colonic epithelial cells, cell-coat glycoproteins are transported to the cell surface in vesicles that originate at the Golgi apparatus. To determine the role of microtubules in the movement of these vesicles the antimicrotubule agent colchicine was injected into mice at several time intervals prior to sacrifice. In the mice that were treated with colchicine for 4.5 h it was observed that the polarity of the cells was disturbed. The Golgi apparatus and nucleus often appeared interchanged in their positions. The glycoprotein-containing vesicles, normally located apically, were sparse in that location, but abundant near the lateral plasma membranes of the cells at the level of the nucleus and Golgi apparatus. Staining by the periodic acid-chromic acid-silver methenamine technique for glycoproteins clearly revealed the reduction of vesicles apically and accumulation of vesicles laterally. The mechanism responsible for the movement of the vesicles to this location is unclear. It is suggested that the accumulation of vesicles in the lateral region may reflect some hindrance in the fusion of the vesicles with the lateral cell membranes.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 211 (1980), S. 449-463 
    ISSN: 1432-0878
    Keywords: Ependyma ; Choroid plexus ; Leptomeninges ; Ultrastructure ; Bullfrog ; Tela choroidea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The lateral areas of the rhombencephalic tela of the bullfrog contain long, irregular islands of ependymal cells that are similar in fine structure to the epithelium of the rhombencephalic choroid plexus. These cells are characterized by apical microvilli, numerous mitochondria and pinocytotic vesicles, and basal infoldings of the plasma membrane. Dorsally a basal lamina and varying amounts of collagen occur. The pia mater associated with this ependyma includes two cell types. Fibroblast-like, loosely arranged cells without organized junctions line the subarachnoid space. The most abundant cells of the pia in this area, however, contain numerous intermediate filaments and frequent desmosomes. Caveolae lie along their plasma membranes. Closely organized sheets of similar filament-containing cells are also seen in the arachnoid mater of this animal. These findings demonstrate ependymal cells in the lateral areas of the rhombencephalic tela of the bullfrog that have the essential features of choroid plexus epithelium, with ultrastructural characteristics that suggest transport function. They are, however, usually separated from neighboring, nonfenestrated vessels by several layers of leptomeningeal cells joined by desmosomes. The relationship between structure and function of these cells is enigmatic.
    Type of Medium: Electronic Resource
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  • 6
    Publication Date: 2019-06-28
    Description: A study was conducted to define characteristics of a Regenerative Fuel Cell System (RFCS) for low earth orbit Space Station missions. The RFCS's were defined and characterized based on both an alkaline electrolyte fuel cell integrated with an alkaline electrolyte water electrolyzer and an alkaline electrolyte fuel cell integrated with an acid solid polymer electrolyte (SPE) water electrolyzer. The study defined the operating characteristics of the systems including system weight, volume, and efficiency. A maintenance philosophy was defined and the implications of system reliability requirements and modularization were determined. Finally, an Engineering Model System was defined and a program to develop and demonstrate the EMS and pacing technology items that should be developed in parallel with the EMS were identified. The specific weight of an optimized RFCS operating at 140 F was defined as a function of system efficiency for a range of module sizes. An EMS operating at a nominal temperature of 180 F and capable of delivery of 10 kW at an overall efficiency of 55.4 percent is described. A program to develop the EMS is described including a technology development effort for pacing technology items.
    Keywords: ENERGY PRODUCTION AND CONVERSION
    Type: NASA-CR-174802 , FCR-6128 , NAS 1.26:174802
    Format: application/pdf
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  • 7
    Publication Date: 2019-07-13
    Description: Design parameters for two large area, low cost solar cells are presented, and electron irradiation testing, thermal alpha testing, and cell processing are discussed. The devices are a 2 ohm-cm base resistivity silicon cell with an evaporated aluminum reflector produced in a dielectric wraparound cell, and a 10 ohm-cm silicon cell with the BSF/BSR combination and a conventional contact system. Both cells are 5.9 x 5.9 cm and require 200 micron thick silicon material due to mission weight constraints. Normalized values for open circuit voltage, short circuit current, and maximum power calculations derived from electron radiation testing are given. In addition, thermal alpha testing values of absorptivity and emittance are included. A pilot cell processing run produced cells averaging 14.4% efficiencies at AMO 28 C. Manufacturing for such cells will be on a mechanized process line, and the area of coverslide application technology must be considered in order to achieve cost effective production.
    Keywords: ENERGY PRODUCTION AND CONVERSION
    Type: Photovoltaic Specialists Conference; May 12, 1981 - May 15, 1981; Kissimmee, FL
    Format: text
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  • 8
    Publication Date: 2019-06-28
    Description: A process sequence for a large area ( or = 25 sq. cm) silicon solar cell was investigated. Generic cell choice was guided by the expected electron fluence, by the packing factors of various cell envelope designs onto each panel to provide needed voltage as well as current, by the weight constraints on the system, and by the cost goals of the contract.
    Keywords: ENERGY PRODUCTION AND CONVERSION
    Type: NASA-CR-167725 , NAS 1.26:167725
    Format: application/pdf
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