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  • 1
    Electronic Resource
    Electronic Resource
    Heterotrophic growth of Thiobacillus A2 on sugars and organic acids (1977)
    Springer
    Archives of microbiology 113 (1977), S. 257-264 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus A2 ; Facultative heterotrophy ; Diauxic growth ; Sugar metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thiobacillus A2 grew on a number of organic acids, pentoses, hexoses and α-linked disaccharides, but not on β-linked disaccharides or galactosides. Growth was slow on glucose, although fast-growing strains were selectively isolated. Additive growth rates occurred on glucose and galactose; growth on glucose with fructose, pyruvate or gluconate was biphasic rather than diauxic; fructose was used preferentially over glucose; slow growth on glucose was accelerated by some disaccharides; growth on acetate, fumarate or succinate with glucose gave diauxic growth with preferential use of the acid and repression of glucose incorporation. Acetate and succinate tended to be used preferentially even with cultures grown on them in mixture with fructose or sucrose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492033
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  • 2
    Electronic Resource
    Electronic Resource
    Simultaneous operation of three catabolic pathways in the metabolism of glucose by Thiobacillus A2 (1977)
    Springer
    Archives of microbiology 113 (1977), S. 265-274 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus A2 ; Glucose metabolism ; Regulation ; Enzymology ; Radiorespirometry ; Multiple catabolic pathways
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Enzymes essential to the operation of the Embden-Meyerhof glycolytic pathway, the Entner-Doudoroff pathway and oxidative pentose phosphate pathway were present in Thiobacillus A2 grown on glucose and other sugars. Radiorespirometry under various conditions with Thiobacillus A2 oxidising glucose specifically labelled with 14C in carbon atoms 1, 2, 3, 3+4, 6 or universally labelled demonstrated the simultaneous operation of the Embden-Meyerhof (48%), Entner-Doudoroff (28%), and pentose phosphate (24%) pathways in release of carbon dioxide from glucose. Growth on succinate, or autotrophically on formate or thiosulphate resulted in repression of most enzymes of the pathways, but high aldolase levels were retained indicating its role in gluconeogenesis and the Calvin cycle. Different fructose diphosphatase activities were found in succinate- and thiosulphate-grown organisms. The results indicate that all three major catabolic pathways for glucose function in Thiobacillus A2 grown on sugars. Thiobacillus acidophilus showed a different radiorespirometric pattern and apparently used the Entner-Doudoroff (64.5%) and pentose phosphate (35.5%) pathways, but showed unusually high release of carbon atom 6, as was also found for T. ferrooxidans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492034
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  • 3
    Electronic Resource
    Electronic Resource
    Glucose catabolism by Thiobacillus A2 grown in chemostat culture under carbon or nitrogen limitation (1979)
    Springer
    Archives of microbiology 122 (1979), S. 307-312 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus A2 ; Radiorespirometry ; Chemostat culture ; Glucose metabolism ; Multiple metabolic pathways ; Metabolic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thiobacillus A2 was grown in glucose- or ammonium-limited chemostats and relative contributions of the Embden-Meyerhof (EM), Entner-Doudoroff (ED) and pentose phosphate (PP) pathways to glucose catabolism estimated by 14C-glucose radiorespirometry. In fast growing strain GFI, the EM pathway predominated (41–79%) under all growth conditions with the PP pathway contributing 18–30%. The ED pathway was apparently absent under some conditions of glucose limitation. In contrast, wild type Thiobacillus A2 exhibited predominance of the EM pathway (43–48%) under ammonium-limitation but apparent predominance of the PP pathway (43–55%) under glucose-limitation, although all three pathways were calculated to operate. Under some conditions of glucose limitation the EM pathway was possibly considerably depressed. No clear pattern of response of the three pathways to altered environmental conditions could be deduced, although marked change in pathway activities were obviously induced. Growth yield was apparently unaffected by variation in pathways. The problems of interpreting such complex radiorespirometric data are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411296
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  • 4
    Electronic Resource
    Electronic Resource
    Regulation of glucose catabolism in Thiobacillus A2 grown in the chemostat under dual limitation by succinate and glucose (1980)
    Springer
    Archives of microbiology 128 (1980), S. 91-97 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus A2 ; Chemostat culture ; Glucose oxidation ; Succinate metabolism ; Dual substrate limitation ; Metabolic regulation ; Radiorespirometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In contrast to its diauxic behaviour in batch culture, Thiobacillus A2 grew in chemostat culture using glucose and succinate as dual limiting substrates. Biomass production under dual limitations was the sum of that on single substrates with each substrate being oxidized and assimilated to similar extents in single and dual substrate-limited cultures. In glucose and glucose + succinate-limited cultures glucose was oxidized largely by the Entner-Doudoroff and pentose phosphate pathways, but other mechanisms also contributed and the ratios of pathways depended on substrate ratios and the previous substrate-history of the culture. Variations in specific activities of enzymes of carbohydrate metabolism following switches from single to mixed substrates were considerable, ranging from fourfold for fructose diphosphate aldolase to more than 200-fold for hexokinase, fructose diphosphatase, glucose 6-phosphate and 6-phosphogluconate dehydrogenases. Changes in specific activities occurred only over prolonged time periods in the chemostat, probably reflecting low concentrations of free substrates in carbon-limited cultures and consequent low levels of catabolite repression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422311
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