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  • 1
    ISSN: 1570-7458
    Keywords: Insecta ; Tephritidae ; attractant ; behavior ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Male Mediterranean fruit flies,Ceratitis capitata (Wiedemann), produce volatile chemicals thought to be attractive sex pheromones. We demonstrated for the first time that male odor elicits upwind flight and zigzagging upwind flight patterns in mature unmated females. Such flight patterns indicate the mechanisms involved in female location of the pheromone source and arrival at that source. Similar female oriented upwind flight responses occurred with a three component blend comprised of ethyl-(E)-octenoate, geranyl acetate, andE,E-alpha farnesene. These findings clarify the role of male sex pheromone in mate-location strategies in this species and provide new bioassay criteria for evaluating attraction responses to male pheromone and synthetic blends.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-1561
    Keywords: Toxotrypana curvicauda ; Diptera ; Tephritidae ; papaya fruit fly ; male sex pheromone ; pheromone ; 2-methyl-6-vinylpyrazine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A male-produced sex pheromone of the papaya fruit fly,Toxotrypana curvicauda Gerstaecker, was isolated from volatiles collected from air passed over calling males and was identified as 2-methyl-6-vinylpyrazine by comparative gas-liquid chromatographic and spectroscopic evidence. Synthetic 2-methyl-6-vinylpyrazine elicited typical pheromonal responses from unmated mature female flies such as walking, running, and flying in an arena bioassay; flying upwind with a zigzag flight pattern; and hovering in the pheromone plume in a wind-tunnel bioassay. These responses were similar quantitatively and qualitatively to responses to naturally occurring pheromone from calling male papaya fruit flies.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-1561
    Keywords: Insecta ; Diptera ; Tephritidae ; Mediterranean fruit fly ; pheromone ; attractant ; Ceratitis capitata ; ethyl-(E)-3-octenoate ; geranyl acetate ; (E,E)-α-farnesene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three major components, ethyi-(E)-3-octenoate, geranyl acetate, and (E,E)-α-farnesene, emitted as volatiles by laboratory-reared and wild male medflies were collected and analyzed qualitatively and quantitatively. Peak emission of these compounds occurred during the third to fifth hours of the photophase and differences were observed in the ratios of the three components emitted by male laboratory-reared and wild flies. These three compounds were synthesized, and a method was developed to formulate a synthetic blend that released the compounds in a ratio similar to that emitted by wild male medflies. Attractiveness of the blend to female medflies was demonstrated under field conditions by comparing trap catches. Black spherical traps, baited with the synthetic blend to release 1.6 male equivalents, caught significantly more females than blank traps and traps from which the blend released was 0.3, 3.2 or 6.4 male equivalents.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-1561
    Keywords: Bioassay system ; flight tunnel ; fruit flies ; Caribbean fruit fly ; Anastrepha suspensa ; Diptera ; Tephritidae ; pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A bioassay system was developed that permits the testing of various substrates for biological activity in a flight tunnel, while simultaneously collecting a portion of the volatiles from the attractive source for subsequent chemical identification and quantification. Bioassays of the response of virgin female Caribbean fruit flies,Anastrepha suspensa (Loew) (Diptera: Tephritidae), to volatiles released by calling males were conducted in a greenhouse under natural light cycles and fluctuating environmental conditions, similar to those in the field. Using this system, the periodicity of response of the female flies between 1300 and 1845 hr (EST) was tested. Fifty to 75% response occurred between 1700 and 1845 hr. Male pheromone release was greatest between 1500 and 1800 hr. Videotaped records of insects, taken between 1700 and 1800 hr as flies approached and entered the traps, were analyzed to interpret the communicative role of the volatiles released. Significantly more flies landed on and entered the pheromone-emitting trap than the control trap. There was no difference in the amount of time spent on the trap face, an indication that volatiles were attractants. The system described should be of general utility in determination of the attraction of pest fruit flies to suspected attractants.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Journal of chemical ecology 23 (1997), S. 1253-1262 
    ISSN: 1573-1561
    Keywords: Attractants ; Mexican fruit fly ; Diptera ; Tephritidae ; Anastrepha ludens ; host odors ; ammonia ; amines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Combinations of the previously developed attractants CEHO from host fruit aroma and AMPu from volatile metabolites of amino acids were evaluated for attractiveness to gamma-irradiated Mexican fruit flies, Anastrepha ludens, in a citrus orchard. In one experiment, McPhail traps with polyvinyl chloride (PVC) lures loaded with CEHO (10:1:1:50 mixture of 1,8-cineole, ethyl hexanoate, hexanol, and ethyl octanoate) were more attractive than blank traps. McPhail traps with AMPu (10:10:1 mixture of ammonium bicarbonate, methylamine HCl, and putrescine) were more attractive than blank and CEHO traps. Traps containing both AMPu and CEHO lures were less attractive than traps containing AMPu alone. In another experiment, sticky yellow panels and spheres were used to compare attractiveness of the same two attractants in different formulations. Sticky traps baited with membrane formulations of CEHO (10:1:1:10 mixture of the chemicals listed above) were not significantly attractive. Sticky traps with polypropylene tubes containing an agar formulation of AMPu (6:10:1 mixture of ammonium carbonate, methylamine HCl, and putrescine) were more attractive than blank and CEHO baited traps. As in the first experiment, traps with both AMPu and CEHO lures were less attractive than traps with AMPu alone. Results indicate that attractive host-odor volatiles and attractive amino-acid metabolites interact negatively with regard to attractiveness to the Mexican fruit fly.
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