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  • Temperature  (15)
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  • 1
    Publication Date: 2021-05-19
    Description: breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Coldwater ; Temperature ; Fertilization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 2
    Publication Date: 2021-05-19
    Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Population ; Survey ; Coldwater ; Egg ; Temperature ; Sperm ; Motility ; Fertilization
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 51pp.
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  • 3
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25512 | 18721 | 2018-10-05 15:26:26 | 25512 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Breeding has moved beyond the point of simply propagating random stock. Genetic potential can be improved by establishing and crossing genetically defined strains, crossing species and manipulating gametes. Some of these activities require cryopreservation of semen. Cryopreserved sperm could also be a means of exchange of genetic material between locations and populations when transportation of live fish is restricted. In this study we had a survey on rainbow trout sperm quality in Genetic and Breeding Center for Coldwatwr Fishes, Shahid Motahari, Yasouj. Then Cryopreservation trials on rainbow trout (Oncorhynchus mykiss) sperm were carried out using six basic extenders. Egg batches of 25 g were inseminated with semen frozen in five 0.5-ml straws. Thowing temperature of cryopreserved sperm also was tested. In anather trial we investigate different cryoprotectant. The result showd that the best period for sperm collection in this center was from Novamber to January. The best extender based on sperm motility parameters were extender 2 and 1 with 57.2 and 56.9% fertilisatin rates, respectively. So this two extender were used in the further experiments. The best thowing temperathre was 25°C in 30s. Testing different cryoprotectant, adding 10% methanol to extender 1 gave the highest fertilization rate (64.6%) among cryoprotectant that used with this extender. In the other extender (2) adding 5% DMSO and mixture of 5% DMSO and 1% glycerol gave the highest fertilization rates (64.8% and 67.0%, respectively). In conclusion using extender 1 and 2 with mentioned cryoprotectant and thowing rate of 25°C in 30 s was recammended for rainbow trout sperm cryopreservation.
    Keywords: Aquaculture ; Iran ; Yasouj ; Sperm cryopreservation ; Rainbow trout ; Extender ; Cryoprotectant ; Onchorhynchus mykiss ; Breeding ; Genetic ; Coldwater ; Temperature ; Fertilization
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 51
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  • 4
    Publication Date: 2021-05-19
    Description: Due to approaching the method of marine algae (G. corticata) mariculture in the Chabahar waters (located at NE of Oman Sea), a series of feasibility studies were carried out to identify the suitable areas for growth of this algae. In this regard, different regions of shallow waters, intertidal zones, and also natural habitats of them were selected for either collecting sample or as culture regions. During the growth periods in autumn, winter and spring (the culture and plantation were not carried out during summer time synchoronized with monsoon season). Some acceptable results were achieved that indicated the meaningful growth rate of plants in the selected stations. According to the obtained results, at the end of culture period the weight growth was 3 times more and the mean length growth of C. coricata talls was from 68 to 134mm at the end of culture period. On the other hand, this statistical analysis didn't show any significant differences in different seasons from point of weight growth. It should be mentioned that some physical and chemical factors of sea water were measured consist of salinity, temperature, DO., pH, nitrate, phosphate and turbidity.
    Description: Published
    Keywords: Physical ; Chemical ; Gracilaria corticata ; Culture ; Growth ; Coastal waters ; Marine algae ; G. corticata ; Mariculture ; Salinity ; Temperature ; pH ; Algae
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.61-72
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  • 5
    Publication Date: 2021-05-19
    Description: The effect of essential fatty acids (EFA) and vitamin C-enriched Artemia nauplii on growth, survival, and resistance to temperature (high) stress in rainbow trout larvae reared in tanks were investigated. The larvae (average weight 120.43mg±13.5) were fed 6 times daily starting at the onset of exogenous feeding for 1 week. Triplicate groups of fish were offered one of four treatments (1) commercial starter food for rainbow trout، (2) newly hatched Artemia nauplii (unenriched) ،(3) highly unsaturated fatty acid (HUFA) + vitamin C-enriched Artemia nauplii and (4) combination of 10 % HUFA+ vitamin C enriched nauplii and commercial starter food. After 1 week, all groups of fish were switched to the commercial diet for an additional period of 3 weeks. Statistical analysis of growth after the first week and at the end of the experiment, showed that growth of larvae in various treatments was significantly different (P〈0.05). After 4 weeks, the larvae in treatment 3 with the average weight of 657.50 ± 57.93 mg had the highest body weight (P〈0.05). The highest percentage of survival (96%) was observed in treatment 3 (P〈0.05). Proximate compositions of trout larvae after one week feeding with experimental diets showed that the protein in the larvae of treatments 3 and 4 was significantly different compared to other treatments (P〈0.05). The best result of resistance to temperature (up to 24ºC) was observed in larvae reared on treatment 3 with 91.34 ± 1.52 percent (P〈0.05).
    Description: Published
    Keywords: Artemia nauplii ; Rainbow trout ; Essential fatty acid ; Vitamin C ; Oncorhynchus mykiss ; Stress resistance ; Temperature ; Growth ; Survival ; Feeding ; Larvae ; Performance
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.557-569
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  • 6
    Publication Date: 2021-05-19
    Description: Possible effects of varying densities of cultured red algae Gracilaria corticata on algal growth parameters were studied using three density treatments 500, 1000 and 20002 g/m2 in nine culture tanks. We conducted the experiment in April to June 2002 and carried out daily tests of salinity, pH and temperature which were in the range 37-40ppm. 0.5-8.04 and 23-32 degrees centigrade. Epiphytes such as Nitzschia, Navicula and Hypnea and grazers such as Amphipoda, Isopoda and Nereis were detected in the tanks during the experiment. We observed the highest daily growth and net production in treatment one (500g/m2) to be 3.74±0.31 and 38.3±5.4 respectively and the lowest measurements were seen in treatment three (2000 g/m2) recorded as 3.74±0.31 and 38.3±5.4 respectively. Daily growth measurements were significantly different among treatments (P〈0.05). Net production as significantly different between treatment one as a group and other treatments (P 0.05). This was not the case between treatments and three.
    Description: Published
    Keywords: Red algae ; Gracilaria corticata ; Algal growth ; Density ; Salinity ; pH ; Temperature ; Epiphytes ; Nitzschia ; Navicula ; Hypnea ; Growth ; Parameters
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.13-24
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  • 7
    Publication Date: 2021-05-19
    Description: Samples for monthly variation of phytoplankton species were collected in two stations from October 1996 till September 1997. In this study 65 species of phytoplankton related to diatoms, dinoflagellates and blue-green algae were identified. Diatoms represent 44 species (67%) related to 25 genera, followed by 18 species (26%) related to 7 genera dinoflagellates and only 3 genus (7%) belong to the blue-green algae. The diversity of diatoms species mainly composed of Rhizosolenia (12 species) and (6 species) were markedly greater than the diversity of dinoflagellates which were composed Ceratium (11 species). The diversity of both groups were significantly greater than the blue-green algae. Diatoms and dinoflagellates were abundant during periods of low salinity and temperature, meanwhile this situation was vise-versa for blue-green algae with high abundance in warm months.
    Description: Published
    Keywords: Phytoplankton ; Species ; Diatoms ; Dinoflagellates ; Blue-green algae ; Genera ; Diversity ; Rhizosolenia ; Salinity ; Temperature ; Algae
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.29-40
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  • 8
    Publication Date: 2021-05-19
    Description: In order to investigate the effect of different temperature of anesthetic (clove tree) and recover (anesthetic-free water) solutions on recovery process, The anesthetic induction times and recovery times of 540 fingerlings of rainbow trout (Oncorhynchus mykiss) weighing 3.2 ± 0.2 g in anesthesia/ recovery solutions with cross-combined of temperature of 5,11 and 17 °C as experimental treatments (9 treatments) were compared. The anesthesia was induced by170 ppm of aqueous clove (Eugenia caryophyllata) solution. Results showed that both induction and recovery times were adversely temperature-dependent. Recovery times of treatments, related to temperature of anesthetic solutions and their temperature, were significantly different (P 〈 0.05). Our results indicated that mean recovery time (three temperature treatments) of the fish exposed to the lower temperature anesthetic solutions generally decreased opposing with higher anesthetic solutions. The lowest recovery time (61±11.1 seconds) in17°C anesthetic-free water occurred in fishes induced to 5°C anesthetic solution. In contrast, the highest recovery time (523±154 seconds) in 5°C water happened in fishes induced to 17°C anesthetic solution. Warm and cold thermal difference in anesthetic and recover solutions could extend recovery time 1 up to 8 minutes. Regarding to aim and demanded time, by selected favorite temperatures can control anesthesia/or recovery time in order to prevent the use of drug over-dosing.
    Description: Published
    Keywords: Clove tree ; Eugenia caryophyllata ; Rainbow trout ; Oncorhynchus mykiss ; Fish ; Temperature
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.117-123
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  • 9
    Publication Date: 2021-05-19
    Description: The objective of this study was to evaluate 4 levels of feeding defined as 1.ad libitum to satiation, 2.Standard feeding (based on fish size and water temperature), 3.10% less than that of standard feeding and 4.10% more than that of standard feeding on growth, feed conversion rate (FCR) and carcass quality of rainbow trout (Oncorhynchus mykiss) reared in 12 aerated flow-through fiber glass tanks (2500l rearing volume, n=150 fish per tank, body weight average 45 g) comprised of 3 replicates under the condition of brackish water (salinity 8.4 g/l) in Bafgh Fisheries Research Station in center of Iran. The increase in average body weight (BW) and size of fish in different feeding groups was significantly different (P〈0.01) during the experiment (101 days) and the highest average BW and size increase occurred in the group fed ad libitum to satiation. On the contrary, FCR (average±SE: 1.27±0.09) in this group was statistically lower than that of the other groups (P〈0.01). The chemical composition of carcass was significantly influenced by the level of feeding (P〈0.01) and the percent of moisture, crude protein and ash was the lowest in the group fed ad libitum to satiation while the crude fat percent in this group was the highest of all. According to the results obtained in this study, an ad libitum feeding regime is suggested for rainbow trout production in fish culture systems of central desert regions in Iran under the condition of brackish water. However, further investigations are needed to draw firm conclusions, especially in earth pond cultures with different conditions, enriched in live food.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Feeding levels ; Growth ; Rainbow trout ; FCR ; Fiberglass tank ; Brackish water ; Temperature ; Oncorhynchus mykiss ; Weight ; Size ; Culture ; Evaluation ; Level ; Body weight
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 36pp.
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  • 10
    Publication Date: 2021-05-19
    Description: This study was conducted in order to optimizing of the biological condition and enhancing of the harvest efficiency for cultured shrimps of Guater site. Experimental design was consisted of three treatments and each treatment with three replications (totally 9 ponds with 600 m2 area for each pond). After preparing and watering of ponds, the ponds was stocked with shrimp post larves in a density of 0.007±0.001 g (or 20 numbers per m2) per m2. After 35 days, the fingerlings of gray mullet were released to shrimp ponds in densities of 0/100 m2 (T1/), 2/100 m2 (T2) and 4/100 m2 (T3). During experiment, the physicochemical parameters of water (temperature, O2, pH, water transparency and salinity) were measured daily for two times (morning and afternoon). Also, the nitrate, nitrite, ammonia, phosphate and silicate concentrations and BOD5 as well as chlorophyll (a), phytoplanktons and zooplanktons were measured every 15 days one time. To determine the organic values of the bottom sediment of the ponds, monthly sampling was conducted. Health status of shrimps was investigated monthly too. Growth rate, mean weight, survival value, Food conversion ratios (FCR) and total shrimp harvest (Kg) were measured after 107 days rearing in ponds. ANOVA analysis showed significant differences between treatments. The highest (212 Kg, 3533 Kg; weight mean was18.4 g for each shrimp) and lowest (187 Kg, 3116 Kg; weight mean was16.23 g for each shrimp) of harvested shrimps were yielded in T3 and T1 respectively. There was not significant differences between treatments in terms of survival rate (P〉0.05). After 107 days rearing, the highest (1.27 0.2) and lowest (1.2 0.1) of FCR were observed in T3 and T1 respectively. Although, there were no significant differences between T2 and T3 in ammonia, O2, Total organic material, pH and BOD5, but, T2 and T3 had significant differences with T1 in these parameters. In this study, 27 genus of phytoplanktons belonged to Diatoms (Bacillariophyceae), blue-green algal (Cyanophyceae) and dinoflagellates (Dinophyceae) were identified. Diatoms with 20 genus had more abundance than dinoflagellates (6 genus) and blue-green algal (1 genus). Also, 7 orders of zooplanktons including copepod, mollusks, nauplius of crustacean and rhizopoda were identified. Health investigation of T1 (without shrimp) showed more pathogenic pollution (parasites and bacteria) than in treatments with shrimp. In this regard, among bacteria, the Vibrio genus (V.Alginoliticus and V.Fluvialis) had more abundance and among parasites zoothamnium sp and Epistylis sp were identified which more abundance was for zoothamnium sp. In conclusion, our results concluded that mixed culturing of white shrimp and grey mullet in optimum densities is possible and is caused more production of shrimp.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Biological ; Physicochemical ; Experimental ; Litopenaeus vannamei ; Mugil cephalus ; Mixed culturing ; Polyculture ; Earthen ponds ; Shrimp ; Fingerlings ; Gray mullet ; Temperature ; O2 ; pH ; Water transparency ; Salinity ; Chlorophyll a ; Phytoplanktons ; Zooplanktons ; ANOVA ; Diatoms ; Bacillariophyceae ; Blue-green algae ; Cyanophyceae ; Copepod ; Investigation
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 92pp.
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