ISSN:
1612-1112
Keywords:
Microcolumn liquid chromatography
;
Supercritical fluid chromatography
;
Cyclodextrin bonded phase
;
Aromatic compounds
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The analytical potential of coarse-particles (dρ=20μm) of preparative-grade, bonded β-cyclodextrin packing material (CD) was investigated by applying them as the stationary phase for liquid chromatographic (LC) and supercritical fluid chromatographic (SFC) microcolumns. A comparative study of the retention behaviour of a number of polycyclic aromatic hydrocarbons (PAHs) and alkylbenzenes in LC and SFC modes was studied with the CD column. The selectivity of the CD column in different chromatographic modes was also investigated. For a number of selected PAH pairs, the experimentally obtained selectivity data with the CD column was compared with those from octadecylsilica (ODS) columns in the literature. The CD column showed unique selectivity both in normal-phase and reversed-phase LC, as well as in SFC. The observed peculiarities of solute retention in the various chromatographic modes is explained based on the structural characteristics of the CD molecule and the solutes. Effective separation of a good number of isomeric compounds belonging to different chemical classes was realized both in LC and SFC modes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02279476
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