ISSN:
1432-1432
Keywords:
Subunit hybridization
;
Equilibrium dissociation constants
;
Amino acid substitutions
;
Subunit contact domains
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The theoretical basis of the quantitative subunit hybridization technique and its ability to measure evolutionary amino acid substitutions is examined. Homospecific:heterospecific enzyme ratios found after subunit reassociation depend upon K1.2, the equilibrium constant for the dissociation of the heterospecific enzyme. It is shown that if this constant is near the geometric mean of the two homospecific enzyme dissociation constants, as it should be in enzymes whose subunits pair isologously, the quantitative subunit hybridization method will not detect most changes in the subunit contact regions of homologous proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01733266
Permalink
