ISSN:
1432-072X
Keywords:
Key words Nitrogen-fixing bacteria
;
Stringent response
;
ppGpp
;
RelA
;
ATP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Following amino acid or ammonium starvation, ppGpp is accumulated by Rhizobium meliloti strain 1021 but not by R. meliloti strain 41 or Rhizobium tropici. Azorhizobium caulinodans ORS571 produced ppGpp following amino acid deprivation; however, the free-living nitrogen-fixing bacteria Azotobacter vinelandii and Azomonas agilis did not produce ppGpp. Western blot analysis using anti-RelA antibody demonstrated that R. meliloti strain 1021, Azotobacter vinelandii and Azorhizobium caulinodans cross-reacted under conditions that detected RelA in Escherichia coli CF1648. Cross-reaction was not observed in R. meliloti strain 41, R. tropici, or Azomonas agilis. All strains that accumulated ppGpp also produced high intracellular levels of ATP.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050689
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