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  • 1
    Electronic Resource
    Electronic Resource
    Accumulation of ppGpp in symbiotic and free-living nitrogen-fixing bacteria following amino acid starvation (1999)
    Springer
    Archives of microbiology 171 (1999), S. 131-134 
    Details
    ISSN: 1432-072X
    Keywords: Key words Nitrogen-fixing bacteria ; Stringent response ; ppGpp ; RelA ; ATP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Following amino acid or ammonium starvation, ppGpp is accumulated by Rhizobium meliloti strain 1021 but not by R. meliloti strain 41 or Rhizobium tropici. Azorhizobium caulinodans ORS571 produced ppGpp following amino acid deprivation; however, the free-living nitrogen-fixing bacteria Azotobacter vinelandii and Azomonas agilis did not produce ppGpp. Western blot analysis using anti-RelA antibody demonstrated that R. meliloti strain 1021, Azotobacter vinelandii and Azorhizobium caulinodans cross-reacted under conditions that detected RelA in Escherichia coli CF1648. Cross-reaction was not observed in R. meliloti strain 41, R. tropici, or Azomonas agilis. All strains that accumulated ppGpp also produced high intracellular levels of ATP.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050689
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  • 2
    Electronic Resource
    Electronic Resource
    The distribution of and interactions between GABA-immunoreactive and non-immunoreactive processes presynaptic to afferents from campaniform sensilla on the trochanter of the locust leg (1991)
    Springer
    Cell & tissue research 266 (1991), S. 331-341 
    Details
    ISSN: 1432-0878
    Keywords: Campaniform sensilla ; Ganglia, invertebrate ; Sensory projections ; GABA ; Schistocerca gregaria (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Campaniform sensilla on the trochanter of the mesothoracic legs of the locust were backfilled with cobalt salts or horseradish peroxidase for light and electron microscopy. The distribution of the terminal branches of afferent neurones in the thoracic ganglia were described from wholemount preparations and from thick slices through the ganglia. Ultrathin sections of identified branches were processed with GABA antibodies using a post-embedding immunogold technique and examined in the electron microscope. Input synapses were observed on fine varicose branches in all regions of the terminal arborisations close to the sites of afferent output. The major branches neither make nor receive synapses. Seventy-two percent of the input synapses are made by processes immunoreactive for GABA. Immunoreactive and non-immunoreactive processes synapse onto afferent terminals in close proximity. In some instances GABA-immunoreactive processes presynaptic to an afferent are also presynaptic to a non-immunoreactive presynaptic processes strongly suggesting that different presynaptic influences can interact directly with each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318189
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