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  • 1
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 195-199 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Linkage map ; Helianthus annuus ; Sunflower cDNAs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed linkage map of Helianthus annuus was constructed based on segregation at 234 RFLP loci, detected by 213 probes, in an F2 population of 289 individuals (derived from a cross between the inbred lines HA89 and ZENB8). The genetic markers covered 1380 centiMorgans (cM) of the sunflower genome and were aranged in 17 linkage groups, corresponding to the haploid number of chromosomes in this species. One locus was found to be unlinked. Although the average interval size was 5.9 cM, there were a number of regions larger than 20 cM that were devoid of markers. Genotypic classes at 23 loci deviated significantly from the expected ratios (1∶2∶1 or 3∶1), all showing a reduction in the ZENB8 homozygous class. The majority of these loci were found to map to four regions on linkage groups G, L and P.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220877
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular marker analysis of Helianthus annuus L. 1. Restriction fragment length polymorphism between inbred lines of cultivated sunflower (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 435-441 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Helianthus annuus ; Cytoplasmic male sterility (CMS) ; Restorer ; Maintainer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract cDNA and PstI genomic clones have been used to assess levels of restriction fragment length polymorphism (RFLP) in Helianthus annuus and to determine the inter-relationships between a diverse set of 24 inbred lines. Of the cDNA clones screened 45% were useful as RFLP probes, compared to less than 20% from the PstI library, which showed high levels of redundancy for high copy sequences. Fifty-seven low-copy DNA probes (23 PstI and 34 cDNA clones) were used to fingerprint 12 maintainer (B) lines and 12 restorer (R) lines. The average number of RFLP variants per probe was found to be 3.2, with a mean polymorphic index of 0.49, indicating that high levels of nuclear DNA polymorphism are to be found in cultivated sunflower. Cluster and principal coordinate analysis of the fingerprinting data clearly separated the maintainer and restorer lines, but there was a degree of association between 2 unbranched R-lines and the B-line germ plasm pool.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225378
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    Paper (German National Licenses)
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  • 3
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    Incompressible boundary-layer stability analysis of LFC experimental data for sub-critical Mach numbers (1986)
    In:  CASI
    Details
    Publication Date: 2019-06-28
    Description: An incompressible boundary-layer stability analysis of Laminar Flow Control (LFC) experimental data was completed and the results are presented. This analysis was undertaken for three reasons: to study laminar boundary-layer stability on a modern swept LFC airfoil; to calculate incompressible design limits of linear stability theory as applied to a modern airfoil at high subsonic speeds; and to verify the use of linear stability theory as a design tool. The experimental data were taken from the slotted LFC experiment recently completed in the NASA Langley 8-Foot Transonic Pressure Tunnel. Linear stability theory was applied and the results were compared with transition data to arrive at correlated n-factors. Results of the analysis showed that for the configuration and cases studied, Tollmien-Schlichting (TS) amplification was the dominating disturbance influencing transition. For these cases, incompressible linear stability theory correlated with an n-factor for TS waves of approximately 10 at transition. The n-factor method correlated rather consistently to this value despite a number of non-ideal conditions which indicates the method is useful as a design tool for advanced laminar flow airfoils.
    Keywords: AERODYNAMICS
    Type: NASA-CR-3999 , NAS 1.26:3999
    Format: application/pdf
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    NASA TECHNICAL REPORTS
  • 4
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    A unique measurement technique to study laminar-separation bubble characteristics on an airfoil (1987)
    In:  Other Sources
    Details
    Publication Date: 2019-06-28
    Description: A 'nonintrusive', multielement heat-transfer sensor was designed to study laminar-separation bubble characteristics on a NASA LRN (1)-1010 low-Reynolds number airfoil. The sensor consists of 30 individual nickel films, vacuum-deposited on a thin substrate (0.05 mm) that was bonded to the airfoil model with the sensor array placed streamwise on the airfoil upper surface. Experiments were conducted on a 15-cm chord model in the 50,000-300,000 chord Reynolds number range. Time history as well as spectral analysis of signals from surface film gauges were simultaneously obtained to determine the location of laminar separation and the subsequent behavior of the separated shear layer. In addition to the successful determination of laminar separation, a new phenomenon involving a large phase shift in dynamic shear stresses across the separation and reattachment points was observed.
    Keywords: AERODYNAMICS
    Type: AIAA PAPER 87-1271
    Format: text
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    NASA TECHNICAL REPORTS
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