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Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower

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Abstract

A detailed linkage map of Helianthus annuus was constructed based on segregation at 234 RFLP loci, detected by 213 probes, in an F2 population of 289 individuals (derived from a cross between the inbred lines HA89 and ZENB8). The genetic markers covered 1380 centiMorgans (cM) of the sunflower genome and were aranged in 17 linkage groups, corresponding to the haploid number of chromosomes in this species. One locus was found to be unlinked. Although the average interval size was 5.9 cM, there were a number of regions larger than 20 cM that were devoid of markers. Genotypic classes at 23 loci deviated significantly from the expected ratios (1∶2∶1 or 3∶1), all showing a reduction in the ZENB8 homozygous class. The majority of these loci were found to map to four regions on linkage groups G, L and P.

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References

  • Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898

    Google Scholar 

  • Berry ST, Allen RA, Barnes SR, Caligari PDS (1994) Molecular marker analysis of Helianthus annuus L. 1. Restriction fragment length polymorphisms between inbred lines of cultivated sunflower. Theor Appl Genet 89:435–441

    Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in human using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    CAS  PubMed  Google Scholar 

  • Choumane W, Heizmann P (1988) Structure and variability of nuclear ribosomal genes in the genus Helianthus. Theor Appl Genet 76:481–489

    Google Scholar 

  • Diers BW, Keim P, Fehr WR, Shoemaker RC (1992) RFLP analysis of soybean seed protein and oil content. Theor Appl Genet 83:608–612

    Google Scholar 

  • Edwards MD, Stuber CW, Wendel JF (1987) Molecular marker-facilitated investigations of quantitative trait loci in maize. I. Numbers, genomic distribution, and types of gene action. Genetics 116:113–125

    CAS  PubMed  Google Scholar 

  • Feinberg AP, Vogelstein B (1983) A technique for radiolabelling endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    CAS  PubMed  Google Scholar 

  • Gebhardt RE, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75

    Google Scholar 

  • Gentzbittel L, Perrault A, Nicolas P (1992) Molecular phylogeny of the Helianthus genus, based on nuclear restriction fragment length polymorphism (RFLP). Mol Biol Evol 9:872–892

    Google Scholar 

  • Graner A, Jahoor A, Schondelmaier J, Seidler H, Pillen K, Fischbeck G, Wenzel G (1991) Construction of an RFLP map of barley. Theor Appl Genet 83:250–256

    Google Scholar 

  • Heiser CB, Smith DM (1955) New chromosome numbers in Helianthus and related genera. Proc Ind Acad Sci 64:250–253

    Google Scholar 

  • Helentjaris T (1987) A genetic map for maize based on RFLPs. Trends Genet 3:217–221

    Google Scholar 

  • Helentjaris T, Weber D, Wright S (1988) Identification of the genomic location of duplicate nucleotide sequences in maize by analysis of restriction fragment length polymorphisms. Genetics 118:353–363

    CAS  Google Scholar 

  • Heun M, Kennedy AE, Anderson JA, Lapitan NLV, Sorrells ME, Tanksley SD (1991) Construction of a restriction fragment length polymorphism map for barley (Hordeum vulgare). Genome 34:437–447

    Google Scholar 

  • Jackson RC, Murray BG (1983) Colchicine induced quadrivalent formation in Helianthus: Evidence for ancient polyploidy. Theor Appl Genet 64:219–222

    Google Scholar 

  • Kahler AL, Lay CL (1985) Genetics of electrophoretic variants in he annual sunflower. J Hered 76:335–340

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Krauter R, Steinmetz A, Friedt W (1991) Efficient interspecific hybridisation in the genus Helianthus via “embryo rescue” and characterisation of the hybrids. Theor Appl Genet 82:521–525

    Google Scholar 

  • Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    CAS  PubMed  Google Scholar 

  • Landry BS, Kesseli RV, Farrara B, Michelmore R (1987) A genetic linkage map of lettuce (Lactuca sativa L.) with restriction fragment length polymorphism, isozyme, disease resistance and morphological markers. Genetics 116:331–337

    Google Scholar 

  • Landry BS, Hubert N, Etoh T, Harada JJ, Lincoln SE (1991) A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genomics 34:543–552

    Google Scholar 

  • Leclerq P (1966) Une sterilite male utilisable pour la production d'hybrids simples de tournesol. Ann Amelior Plant 16:135–144

    Google Scholar 

  • Liu YG, Tsunewaki K (1991) Restriction fragment length polymorphism (RFLP) analysis in wheat. II. Linkage maps of the RFLP sites in common wheat. Jpn J Genet 66:617–633

    Google Scholar 

  • McCouch SR, Kochert G, Yu ZH, Wang ZY, Khush GS, Coffman WR, Tanksley SD (1988) Molecular mapping of rice chromosomes. Theor Appl Genet 76:815–829

    CAS  Google Scholar 

  • Miller JF (1992) Update on inheritance of sunflower characteristics. In: Proc 13TH Int Sunflower Conf, vol. 2, Pisa, Italy, pp 905–945

  • Nodari RO, Tsai SM, Gilbertson RL, Gepts P (1993) Towards an integrated map of common bean. 2. Development of an RFLP-based linkage map. Theor Appl Genet 85:513–520

    Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Peterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors by using a complete linkage map of restriction fragment length polymorphisms. Nature 335:721–726

    Article  CAS  PubMed  Google Scholar 

  • Rieseberg LH, Choi H, Chan R, Spore C (1993) Genomic map of a diploid hybrid species. Heredity 70:285–293

    Google Scholar 

  • Rogers SO, Bendich AJ (1985) Extracting DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76

    Google Scholar 

  • Shoemaker RC, Guffy RD, Lorenzen LL, Specht JE (1992) Molecular genetic mapping of soybean: Map utilization. Crop Sci 32:1091–1098

    Google Scholar 

  • Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64

    Google Scholar 

  • Song KM, Suzuki JY, Slocum MK, Williams PH, Osborn TC (1991) A linkage map of Brassica rapa (syn. campestris) based on restriction fragment length polymorphism loci. Theor Appl Genet 82:296–304

    Google Scholar 

  • Vallejos CE, Sakiyama NS, Chase CD (1992) A molecular markerbased map of Phaseolus vulgaris L. Genetics 131:733–740

    Google Scholar 

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Authors and Affiliations

  1. ZENECA Seeds, Plant Biotechnology, Jealott's Hill Research Station, RG12 6EY, Bracknell, Berkshire, UK

    S. T. Berry, C. C. Hanfrey & P. Challis

  2. Research Department, ZENECA Seeds-Argentina, 7620, Balcarce, Argentina

    A. J. Leon

  3. ZENECA Seeds-SES, Industriepark 15, B-3300, Tienen, Belgium

    A. Burkholz & S. R. Barnes

  4. Research Department, ICI Seeds, 50244, Slater, IA, USA

    G. K. Rufener

  5. Department of Agronomy, Iowa State University, 50011, Ames, IA, USA

    M. Lee

  6. Department of Agricultural Botany, School of Plant Sciences, University of Reading, Whiteknights, P.O. Box 221, RG6 2AS, Reading, Berkshire, UK

    P. D. S. Caligari

Authors
  1. S. T. Berry
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  2. A. J. Leon
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  3. C. C. Hanfrey
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  4. P. Challis
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  5. A. Burkholz
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  6. S. R. Barnes
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  7. G. K. Rufener
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  8. M. Lee
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  9. P. D. S. Caligari
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Communicated by J. W. Snape

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Berry, S.T., Leon, A.J., Hanfrey, C.C. et al. Molecular marker analysis of Helianthus annuus L. 2. Construction of an RFLP linkage map for cultivated sunflower. Theoret. Appl. Genetics 91, 195–199 (1995). https://doi.org/10.1007/BF00220877

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  • DOI: https://doi.org/10.1007/BF00220877

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