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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 21 (1996), S. 309-313 
    ISSN: 1432-0789
    Keywords: Nodulation ; Competitiveness ; Rhizobium sp. (Cicer) ; lacZ fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ+ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ+ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ+) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1997), S. 96-101 
    ISSN: 1432-0789
    Keywords: Pyrites ; Pyrite oxidation ; Gypsum Alkali soil ; Reclamation ; Soluble sulfur Welland rice ; Wheat ; Thiobacillus thioxidans ; Thiobacillus ferrooxidans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We evaluated the effect of agricultural-grade (AG) pyrites (total sulfur 22%) varying in water-soluble sulfur (1–8%) and gypsum on the soil properties and yields of rice and wheat in alkali soils during the years 1993–1995 at the Gudha and Saraswati experimental farms at the Central Soil Salinity Research Institute, Karnal, India. Gypsum and pyrites were applied on the basis of gypsum requirement (GR) of the soils. Results showed that the efficiency of AG pyrites in decreasing soil pH and exchangeable sodium percentage (ESP) and increasing crop yields was dependent on their water-soluble sulfur content at the time of application to the field. Pyrites with 5.5% and 8% soluble sulfur were as effective as gypsum. The freshly mined pyrite (water-soluble S 1%) was found to be inefficient in reclaiming alkali soils. We also explored the possibility of increasing the water-soluble sulfur content of pyrite by optimizing its storage conditions. When pyrite (1% water-soluble S) was stored under moist conditions by sprinkling water over the bags under a rain shelter, there was an enrichment of indigenous iron- and sulfur-oxidizing bacteria of pyrite, and the water-soluble sulfur increased to 5% within a period of 6 months. However no such increase occurred when pyrite was stored dry. We conclude that the soluble sulfur content of pyrite increased during its storage under moist conditions and should be between 6% and 8% at the time of its application to the field.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 21 (1996), S. 309-313 
    ISSN: 1432-0789
    Keywords: Key words Nodulation ; Competitiveness ; Rhizobium sp. (Cicer) ; lacZ fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We constructed lacZ fusions in Rhizobium sp. (Cicer) by random Tn5-lacZ mutagenesis. The lacZ+ fusants formed blue colonies on a Rhizobial minimal medium containing 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal). Rhizobium sp. (Cicer) fusant HSL-2 was identified in nodules and soil in a mixed population on the basis of the lacZ+ phenotype. Nodule occupancy of inoculated Rhizobium sp. (Cicer) HSL-2 (lacZ+) was assessed by directly streaking the nodule sap on X-gal plates. This method revealed differences between rhizobia carrying identical antibiotic markers. The rhizobial population in soil was estimated by direct plate counts using a medium containing X-gal. Introduction of lacZ into the Rhizobium sp. thus provided a simple and direct method for identifying strains from nodules and soil.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 24 (1996), S. 96-101 
    ISSN: 1432-0789
    Keywords: Key words Pyrites ; Pyrite oxidation ; Gypsum ; Alkali soil ; Reclamation ; Soluble sulfur ; Wetland rice ; Wheat ; Thiobacillus thioxidans ; Thiobacillus ferrooxidans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We evaluated the effect of agricultural-grade (AG) pyrites (total sulfur 22%) varying in water-soluble sulfur (1–8%) and gypsum on the soil properties and yields of rice and wheat in alkali soils during the years 1993–1995 at the Gudha and Saraswati experimental farms at the Central Soil Salinity Research Institute, Karnal, India. Gypsum and pyrites were applied on the basis of gypsum requirement (GR) of the soils. Results showed that the efficiency of AG pyrites in decreasing soil pH and exchangeable sodium percentage (ESP) and increasing crop yields was dependent on their water-soluble sulfur content at the time of application to the field. Pyrites with 5.5% and 8% soluble sulfur were as effective as gypsum. The freshly mined pyrite (water-soluble S 1%) was found to be inefficient in reclaiming alkali soils. We also explored the possibility of increasing the water-soluble sulfur content of pyrite by optimizing its storage conditions. When pyrite (1% water-soluble S) was stored under moist conditions by sprinkling water over the bags under a rain shelter, there was an enrichment of indigenous iron- and sulfur-oxidizing bacteria of pyrite, and the water-soluble sulfur increased to 5% within a period of 6 months. However no such increase occurred when pyrite was stored dry. We conclude that the soluble sulfur content of pyrite increased during its storage under moist conditions and should be between 6% and 8% at the time of its application to the field.
    Type of Medium: Electronic Resource
    Location Call Number Expected Availability
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