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  • 1
    Digitale Medien
    Digitale Medien
    DNA variation in tissue-culture-derived rice plants (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 673-679 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Rice ; Tissue culture ; Somaclonal variation ; RFLP ; Methylation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Regenerants of rice were examined by RFLP analysis to determine the occurrence and extent of somaclonal variation. DNA polymorphisms were observed both among plants regenerated from different callus cultures as well as among sibling plants derived from a single callus. Regardless of the basal medium, a higher degree of genetic instability was found among plants regenerated from callus cultures maintained for longer incubation periods (67 days) than among those from shorter incubation periods (28 days). Detailed analysis showed that in several regenerants, there was a close correlation among those plants exhibiting DNA rearrangements and those with apparent methylation changes. Such alterations were observed with both structural and housekeeping genes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224228
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    RFLP analysis of Zea mays callus cultures and their regenerated plants (1991)
    Springer
    Theoretical and applied genetics 81 (1991), S. 227-232 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Callus cultures ; RFLP ; Zea mays ; Oryza sativa ; Regeneration ; Somaclonal variation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Tissue culture of the Zea mays inbred line A188 resulted in the regeneration of plants having a high level of phenotypic variation compared to seed-grown control plants. To determine how such variation was induced and whether this could be related to specific in vitro culture methods, callus cultures were established and maintained on different, commonly used culture media. Plants were regenerated and the genomic DNA of callus cultures and regenerants analysed for RFLP differences. The results show that regardless of the gene probe used, callus formation resulted in significant deviations from the DNA pattern normally found in seed-grown control plants. Alterations in gene copy number also occurred. As differentiation and organogenesis began, the level of DNA variation fell, and most of the regenerated plants showed a genetic similarity to the controls; those with RFLP differences were the somaclonal variants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00215727
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Molecular changes in protoplast-derived rice plants (1990)
    Springer
    Molecular genetics and genomics 223 (1990), S. 324-328 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Rice ; Protoplast ; RFLP ; Methylation ; Polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary To determine whether regeneration of rice plants from protoplast culture induces DNA polymorphisms, progeny plants from direct regenerants of such cultures were examined for restriction fragment length polymorphisms (RFLP analysis). Significantly increased levels of DNA polymorphism were found compared with those in non-tissue culture control plants. Analysis with gene sequences representative of different functional domains, revealed that such polymorphisms are apparently widespread and not associated with any particular region. Analysis by comparative digestion with both methylation-sensitive and insensitive restriction enzymes revealed that methylation changes cannot be regarded as a major factor in the induction of these DNA polymorphisms.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00265070
    Standort Signatur Erwartet Verfügbarkeit
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    Artikel (Nationallizenzen)
    Volltext
  • 4
    facet.materialart.
    Unbekannt
    Origins of serotonin innervation of forebrain structures (1977)
    In:  Other Sources
    Details
    Publikationsdatum: 2011-08-17
    Beschreibung: The tryptophan hydroxylase activity and high-affinity uptake of (3H) serotonin ((3H)5-HT) were measured in five discrete brain regions of rats following lesions of the dorsal or median raphe nuclei. Dorsal raphe lesions reduced enzyme and uptake activity in the striatum only. Median raphe lesions reduced activities in the hippocampus, septal area, frontal cortex, and, to a lesser extent, in the hypothalamus. These data are consistent with the suggestion that the dorsal and median raphe nuclei are the origins of two separate ascending serotonergic systems - one innervating striatal structures and the other mesolimbic structures, predominantly. In addition, the data suggest that measurements of high-affinity uptake of (3H)5-HT may be a more reliable index of innervation than either 5-HT content or tryptophan hydroxylase activity.
    Schlagwort(e): LIFE SCIENCES (GENERAL)
    Materialart: Experimental Neurology; 56; July 197
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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    NASA TECHNICAL REPORTS
  • 5
    facet.materialart.
    Unbekannt
    Histamine H2 receptor - Involvement in gastric ulceration (1976)
    In:  Other Sources
    Details
    Publikationsdatum: 2011-08-16
    Beschreibung: The involvement of the H1 and H2 receptors for histamine in the pathogenesis of gastric ulcers was investigated in rats. Metiamide, an H2 receptor antagonist, reliably reduced ulceration produced by stress alone or by a combination of stress and aspirin. In contrast, pyrilamine, which blocks only the H1 receptor, was without effect under these same conditions. The results support the hypothesis that histamine mediates both stress and stress plus aspirin induced ulceration by a mechanism involving the H2 receptor.
    Schlagwort(e): LIFE SCIENCES (GENERAL)
    Materialart: Life Sciences; 18; 1976
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
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    NASA TECHNICAL REPORTS
  • 6
    facet.materialart.
    Unbekannt
    Synaptosomal uptake of hypothalamic monoamines and recovery of pituitary-adrenal activity following medial forebrain bundle lesions in rats (1979)
    In:  Other Sources
    Details
    Publikationsdatum: 2011-08-17
    Schlagwort(e): LIFE SCIENCES (GENERAL)
    Materialart: Neuroendocrinology; 28; 1979
    Format: text
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    NASA TECHNICAL REPORTS
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