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  • RFLP  (7)
  • Springer  (7)
  • 1995-1999  (7)
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  • Springer  (7)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Tagging and mapping the thermo-sensitive genic male-sterile gene in rice (Oryza sativa L.) with molecular markers (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1111-1114 
    Details
    ISSN: 1432-2242
    Keywords: Rice TGMS gene ; RAPD ; RFLP ; Molecular markers ; Gene tagging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The thermo-sensititve genic male-sterile (TGMS) gene in rice can alter fertility in response to temperature and is useful in the two-line system of hybrid rice production. However, little is known about the TGMS gene at the molecular level. The objective of this study was to identify molecular markers tightly linked with the TGMS gene and to map the gene onto a specific rice chromosome. Bulked segregant analysis of an F2 population from 5460s (a TGMS mutant line) x ‘Hong Wan 52’ was used to identify RAPD markers linked to the rice TGMS gene. Four hundred RAPD primers were screened for polymorphisms between the parents and between two bulks representing fertile and sterile plants; of these, 4 primers produced polymorphic products. Most of the polymorphic fragments contained repetitive sequences. Only one singlecopy sequence fragment was found, a 1.2-kb fragment amplified by primer OPB-19 and subsequently named TGMS1.2. TGMS1.2 was mapped on chromosome 8 with a RIL population and confirmed by remapping with a DHL population. Segregation analysis using TGMS1.2 as a probe indicated that TGMS1.2 both consegregated and was lined with the TGMS gene in this population. It is located about 6.7 cM from the TGMS gene. As TGMS1.2 is linked to the TGMS gene, the TGMS gene must be located on chromosome 8.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223928
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  • 2
    Electronic Resource
    Electronic Resource
    RFLP mapping of QTLs for yield and related characters in rice (Oryza sativa L.) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 920-927 
    Details
    ISSN: 1432-2242
    Keywords: Oryza sativa L. ; RFLP ; Yield traits ; QTLs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative triat loci (QTLs) for yield and related traits in rice were mapped based on RFLP maps from two indica/indica F2 populations, Tesanai 2/CB and Waiyin 2/CB. In Tesanai 2/CB, 14 intervals carrying QTLs for eight traits were detected, including 3 for grain weight per plant (GWT), 2 for number of panicles per plant (NP), 2 for number of grains per panicle (NG), 1 for total number of spikelets per panicle (TNS), 1 for spikelet fertility (SF), 3 for 1000-grain weight (TGWT), 1 for spikelet density (SD), and 1 for number of first branches per main panicle. The 3 QTLs for GWT were located on chromosomes 1, 2, and 4, with 1 in each chromosome. The additive effect of the single locus ranged from 2.0 g to 9.1 g. A major gene (np4) for NP was detected on chromosome 4 within the interval of RG143–RG214, about 4cM for RG143, and this locus explained 26.1% of the observed phenotypic variance for NP. The paternal allele of this locus was responsible for reduced panicles per plant (3 panicles per plant). In another population, Waiyin 2/CB, 12 intervals containing QTLs for six of the above-mentioned traits were detected, including 3 for GWT, 2 for each of NP, TNS, TGWT and SD, 1 for SF. Three QTLs for GWT were located on chromosome 1, 4, and 5, respectively. The additive effect of the single locus for GWT ranged from 6.7 g to 8.8 g, while the dominance effect was 1.7–11.5 g. QTL mapping in two populations with a common male parent is compared and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224031
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  • 3
    Electronic Resource
    Electronic Resource
    Construction of RFLP-based maps of foxtail millet, Setaria italica (L.) P. Beauv. (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 31-36 
    Details
    ISSN: 1432-2242
    Keywords: Key words Foxtail millet ; Genetic map ; Primary trisomics ; RFLP ; Setaria italica ; Setaria viridis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An RFLP-based map consisting of 160 loci was constructed in an intervarietal cross of foxtail millet [Setaria italica (L.) P. Beauv.], Longgu 25×Pagoda Flower Green. The map comprises nine linkage groups, which were aligned with the nine foxtail millet chromosomes using trisomic lines, and spans 964 cM. The intraspecific map was compared to an interspecific map, constructed in a S. italica×S. viridis cross. Both the order of the markers and the genetic distances between the loci were highly conserved. Deviations from the expected 1 : 2 : 1 Mendelian segregation ratios were observed in both the intra- and inter-specific populations. The segregation data indicate that chromosome VIII in the Longgu 25×Pagoda Flower Green cross carries a gene that strongly affects gamete fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050705
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  • 4
    Electronic Resource
    Electronic Resource
    RFLP-based estimates of parental contribution to F2- and BC1-derived maize inbreds (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 652-656 
    Details
    ISSN: 1432-2242
    Keywords: Key words Bootstrap ; Genetic drift ; Parental contribution ; RFLP ; Selection ; Zea mays L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Selection and genetic drift during inbreeding may cause differences between the actual and expected proportions of the genome derived by an inbred from each of its parents. We used 70 RFLP loci to determine the frequency and magnitude of deviations from the expected parental contribution among F2- and BC1-derived maize (Zea mays L.) inbreds. Assuming inbreds i and j were the parents of inbred k, the parental contribution of i to k was estimated as p=(Sik−Sij)/ (1−Sij), where Sik and Sij were the average proportions, across the ten linkage groups in maize, of RFLP loci with alleles common to the inbreds in subscript. Bootstrap confidence intervals (CIs) were obtained for p by re-sampling RFLP similarity for each linkage group. Among 62 F2-derived inbreds, 13 had estimates of p that deviated significantly from the expected value of 0.5. One F2-derived inbred obtained p=0.801 of its genome from a parent. Among 34 BC1-derived inbreds, eight had estimates of p that deviated significantly from the expected contribution of 0.75 from the recurrent parent. Two inbreds, both from the same BC1 population, had an estimated p?0.94. The results suggested that selection during backcrossing generally favored the recurrent parent over the donor parent. Among the inbreds with significant deviations from the expected p, the width of 95% CIs with 70 RFLP loci was 〉0.20. Inbreds selfed from the same F2 or BC1 population varied in p, indicating that coefficients of co-ancestry calculated from pedigree records may give erroneous estimates of genetic relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050462
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  • 5
    Electronic Resource
    Electronic Resource
    The structure of the Aegilops tauschii genepool and the evolution of hexaploid wheat (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 657-670 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Phylogeny ; Genetic distance ; Genome ; Introgression ; Allopolyploidy ; RFLP ; Glutenin ; rRNA ; Non-transcribed spacers ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Polymorphism in the lengths of restriction fragments at 53 single-copy loci, the rRNA locus Nor3, and the high-molecular-weight glutenin locus Glu1 was investigated in the D genome of hexaploid Triticum aestivum and that of Aegilops tauschii, the source of the T. aestivum D genome. The distribution of genetic variation in Ae. tauschii suggests gene flow between Ae. tauschii ssp. strangulata and ssp. tauschii in Iran but less in Transcaucasia. The “strangulata” genepool is wider than it appears on the basis of morphology and includes ssp. strangulata in Transcaucasia and southeastern (SE) Caspian Iran and ssp. tauschii in north-central Iran and southwestern (SW) Caspian Iran. In the latter region, Ae. tauschii morphological varieties ‘meyeri’ and ‘typica’ are equidistant to ssp. strangulata in Transcaucasia, and both belong to the “strangulata” genepool. A model of the evolution of Ae. tauschii is presented. On the geographic region basis, the D genomes of all investigated forms of T. aestivum are most closely related to the “strangulata” genepool in Transcaucasia, Armenia in particular, and SW Caspian Iran. It is suggested that the principal area of the origin of T. aestivum is Armenia, but the SW coastal area of the Caspian Sea and a corridor between the two areas may have played a role as well. Little genetic differentiation was found among the D genomes of all investigated free-threshing and hulled forms of T. aestivum, and all appear to share a single D-genome genepool, in spite of the fact that several Ae. tauschii parents were involved in the evolution of T. aestivum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050942
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  • 6
    Electronic Resource
    Electronic Resource
    RFLP mapping of QTLs for yield and related characters in rice (Oryza sativa L.) (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 920-927 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza sativa L. ; RFLP ; Yield traits ; QTLs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Quantitative triat loci (QTLs) for yield and related traits in rice were mapped based on RFLP maps from two indica/indica populations, Tesanai 2/CB and Waiyin 2/CB. In Tesanai 2/CB, 14 intervals carrying QTLs for eight traits were detected, including 3 for grain weight per plant (GWT), 2 for number of panicles per plant (NP), 2 for number of grains per panicle (NG), 1 for total number of spikelets per panicle (TNS), 1 for spikelet fertility (SF), 3 for 1000-grain weight (TGWT), 1 for spikelet density (SD), and 1 for number of first branches per main panicle. The 3 QTLs for GWT were located on chromosomes 1, 2, and 4, with 1 in each chromosome. The additive effect of the single locus ranged from 2.0 g to 9.1 g. A major gene (np4) for NP was detected on chromosome 4 within the interval of RG143-RG214, about 4 cM for RG143, and this locus explained 26.1% of the observed phenotypic variance for NP. The paternal allele of this locus was responsible for reduced panicles per plant (3 panicles per plant). In another population, Waiyin 2/CB, 12 intervals containing QTLs for six of the above-mentioned traits were detected, including 3 for GWT, 2 for each of NP, TNS, TGWT and SD, 1 for SF. Three QTLs for GWT were located on chromosome 1, 4, and 5, respectively. The additive effect of the single locus for GWT ranged from 6.7 g to 8.8 g, while the dominance effect was 1.7–11.5 g. QTL mapping in two populations with a common male parent is compared and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050212
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  • 7
    Electronic Resource
    Electronic Resource
    Comparative analyses of RFLP diversity in landraces of Triticum aestivum and collections of T. tauschii from China and Southwest Asia (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 312-318 
    Details
    ISSN: 1432-2242
    Keywords: Key words Genetic diversity ; Triticum tauschii ; Triticum aestivum ; RFLP ; Landrace wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Chinese accessions of Triticum tauschii and T. aestivum L. from the Sichuan white (SW), Yunnan hulled (YH), Tibetan weedrace (TW), and Xinjiang rice (XR) wheat groups were subjected to RFLP analysis. T. tauschii and landraces of T. aestivum from countries in Southwest Asia were also evaluated. For T. tauschii, a west to east gradient was apparent where the Chinese accessions exhibited less diversity than those from Southwest Asia. Compared to the Southwest Asian gene pool, the Chinese T. tauschii was highly homogeneous giving a low frequency of polymorphic bands (16%) and banding patterns (1.33 per probe) with 75 RFLP probe-HindIII combinations. Accessions of T. tauschii from Afghanistan and Pakistan were genetically more similar to the Chinese T. tauschii than those from Iran. Of 368 bands found for 39 Chinese hexaploid wheat accessions with 63 RFLP probe-HindIII combinations, 28.3% were polymorphic with an average of 2.6 banding patterns per probe and 5.0 bands per genotype. The individual Chinese landrace wheat groups revealed less variation than those from Afghanistan, Iran, and Turkey. When classified into country based groups, however, the diversity level over all Chinese landraces was greater than that of some Southwest Asian landraces, especially those from Afghanistan and Iran . The XR wheat group was genetically distinct from the other three Chinese landrace groups and was more related to the Southwest Asian landraces. The TW group was genetically similar to, but more diverse than, the SW and YH groups. The Chinese landraces had a higher degree of genetic relatedness to the Southwest Asian T. tauschii, particularly to accessions from Iran, rather than to the Chinese T. tauschii. ‘Chinese Spring’ was most related to ‘Chengdu-guang-tou’, a cultivar from the SW wheat group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050742
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