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  • Polymer and Materials Science  (13)
  • Life Sciences (General)  (7)
  • Candida albicans  (6)
  • 1
    Digitale Medien
    Digitale Medien
    Changes in lipid composition during starvation and germ-tube formation inCandida albicans
    Amsterdam : Elsevier
    Experimental Mycology 5 (1981), S. 140-147 
    Details
    ISSN: 0147-5975
    Schlagwort(e): Candida albicans ; germ tubes ; germination ; lipids ; phospholipids ; sterols
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1016/0147-5975(81)90014-1
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  • 2
    Digitale Medien
    Digitale Medien
    The alternate respiratory pathway of Candida albicans (1978)
    Springer
    Archives of microbiology 116 (1978), S. 61-67 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Respiration ; Alternate oxidase ; Cytochrome c oxidase ; Candida albicans ; Cyanide and antimycin A insensitive respiration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Candida albicans contains a cryptic cyanide and antimycin A insensitive respiratory system. This alternate oxidase was found (i) at all growth rates from μ=0.05 to 0.26 in a chemostat culture and (ii) in both mycelial and yeast forms of the organism. Neither chloramphenicol nor cycloheximide prevented the expression of the alternate oxidase. Salicyl-hydroxamic acid was a potent inhibitor of the cyanide insensitive respiration. The respiration of mitochondria grown in the presence of antimycin A was not inhibited by cyanide or antimycin A but was inhibited by salicylhydroxamic acid.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00408734
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  • 3
    Digitale Medien
    Digitale Medien
    Regulation of chitin synthesis during germ-tube formation in Candida albicans (1980)
    Springer
    Archives of microbiology 125 (1980), S. 97-104 
    Details
    ISSN: 1432-072X
    Schlagwort(e): l-glutamine-d-fructose-6-phosphate aminotransferase ; Chitin synthase ; Chitin ; Candida albicans ; Germ-tube formation ; Dimorphism ; Polyoxin D
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The synthesis of chitin during germ-tube formation in Candida albicans may be regulated by the first and last steps in the chitin pathway: namely l-glutamine-d-fructose-6-phosphate aminotransferase and chitin synthase. Induction of germ-tube formation with either glucose and glutamine or serum was accompanied by a 4-fold increase in the specific activity of the aminotransferase. Chitin synthase in C. albicans is synthesized as a proenzyme. N-acetyl glucosamine increased the enzymic activity of the activated enzyme 3-fold and the enzyme exhibited positive co-operativity with the substrate, UDP-N-acetylglucosamine. Although chitin synthase was inhibited by polyoxin D (K i =1.2μM) this antibiotic did not affect germination. During germ-tube formation the total chitin synthase activity increased 1.4-fold and the expressed activity (in vivo activated proenzyme) increased 5-fold. These results could account for the reported 5-fold increase in chitin content observed during the yeast to mycelial transformation.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00403204
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  • 4
    Digitale Medien
    Digitale Medien
    The Effect of Cetylpyridinium Chloride (CPC) on the Cell Surface Hydrophobicity and Adherence of Candida albicansto Human Buccal Epithelial Cells in Vitro (1995)
    Springer
    Pharmaceutical research 12 (1995), S. 1896-1900 
    Details
    ISSN: 1573-904X
    Schlagwort(e): Candida albicans ; cetylpyridinium chloride ; reduced adherence ; reduced cell surface hydrophobicity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract Purpose. This study examined the effects of cetylpyridinium chloride (CPC) on cell surface hydrophobicity (CSH) and adherence of blastospores of Candida albicans(MEN strain) to human buccal epithelial cells (EEC) in vitro. Methods. The effect of CPC treatment of either C. albicans blastospores or BEC on their subsequent adherence was determined using 35SO4 labelled blastospores in association with a Percoll™ gradient. The effects of CPC treatment of blastospores on their CSH was determined using Hydrophobic Interaction Chromatography. Results. Treatment of exponential and stationary phase blastospores with CPC (50 µg mL−1) for 0.5–30 minutes, or with CPC (0.5–50 µg mL−1) for 15 minutes resulted in significant reductions in both blastospore CSH and adherence to BEC in vitro. No correlation was apparent (r 〈 0.8) between reduced CSH and reduced blastospore adherence following treatment with CPC (0.5–50 µg mL−1). Significantly reduced adherence of C. albicans (stationary or exponential growth phases) to human EEC was also observed following treatment of BEC with CPC (50 µg mL−1) for 0.5–30 minutes or with CPC (0.5–50 µg mL−1) for 15 minutes. Antiadherence effects were observed at both sub and super-minimum inhibitory concentrations of CPC. Conclusions. It is suggested that, whilst the ability of CPC to reduce the CSH of C. albicans may contribute to its reduced adherence to human BEC in vitro, reduced CSH is only one of several possible factors that contribute to the observed antiadherence effects.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1016231620470
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  • 5
    Digitale Medien
    Digitale Medien
    Cloning and expression of Candida albicans ADE2 and proteinase genes on a replicative plasmid in C. albicans and in Saccharomyces cerevisiae (1992)
    Springer
    Molecular genetics and genomics 235 (1992), S. 453-457 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Autonomously replicating plasmid ; Proteinase ; Candida albicans
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A plasmid vector (denoted pRC2312) was constructed, which replicates autonomously in Escherichia coli, Saccharomyces cerevisiae and Candida albicans. It contains LEU2, URA3 and an autonomously replicating sequence (ARS) from C. albicans for selection and replication in yeasts, and bla (ampicillin resistance) and ori for selection and replication in E. coli. S. cerevisiae AH22 (Leu−) was transformed by pRC2312 to Leu− at a frequency of 1.41 × 105 colonies per μg DNA. Transformation of C. albicans SGY-243 (Ura-) to Ura+ with pRC2312 resulted in smaller transformant colonies at a frequency of 5.42 × 103 per μg DNA where the plasmid replicated autonomously in transformed cells, and larger transformant colonies at a frequency of 32 per μg DNA, in which plasmid integrated into the genome. Plasmid copy number in yeasts was determined by a DNA hybridization method and was estimated to be 15±3 per haploid genome in S. cerevisiae and 2–3 per genome in C. albicans replicative transformants. Multiple tandem integration occurred in integrative transformants and copy number of the integrated sequence was estimated to be 7–12 per diploid genome. The C. albicans ADE2 gene was ligated into plasmid pRC2312 and the construct transformed Ade− strains of both C. albicans and S. cerevisiae to Ade+. The vector pRC2312 was also used to clone a fragment of C. albicans genomic DNA containing an aspartic proteinase gene. C. albicans transformants harboring this plasmid showed a two-fold increase in aspartic proteinase activity. However S. cerevisiae transformants showed no such increase in proteinase activity, suggesting the gene was not expressed in S. cerevisiae.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00279393
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  • 6
    Digitale Medien
    Digitale Medien
    Isolation and nucleotide sequence of an autonomously replicating sequence (ARS) element functional in Candida albicans and Saccharomyces cerevisiae (1990)
    Springer
    Molecular genetics and genomics 221 (1990), S. 210-218 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Candida albicans ; Autonomously replicating sequence (ARS) ; 5S rRNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary An 8.6-kb fragment was isolated from an EcoRI digest of Candida albicans ATCC 10261 genomic DNA which conferred the property of autonomous replication in Saccharomyces cervisiae on the otherwise non-replicative plasmid pMK155 (5.6 kb). The DNA responsible for the replicative function was subcloned as a 1.2-kb fragment onto a non-replicative plasmid (pRC3915) containing the C. albicans URA3 and LEU2 genes to form plasmid pRC3920. This plasmid was capable of autonomous replication in both S. cerevisiae and C. albicans and transformed S. cerevisiae AH22 (leu2 −) to Leu+ at a frequency of 2.15 × 103 transformants per pg DNA, and transformed C. albicans SGY-243 (Δura3) to Ura+ at a frequency of 1.91 × 103 transformants per μg DNA. Sequence analysis of the cloned DNA revealed the presence of two identical regions of eleven base pairs (5′TTTTATGTTTT3′) which agreed with the consensus of autonomously replicating sequence (ARS) cores functional in S. cerevisiae. In addition there were two 10/11 and numerous 9/11 matches to the core consensus. The two 11/11 matches to the consensus, CaARS1 and CaARS2, were located on opposite strands in a non-coding AT-rich region and were separated by 107 bp. Also present on the C. albicans DNA, 538 by from the ARS cores, was a gene for 5S rRNA which showed sequence homology with several other yeast 5S rRNA genes. A sub-fragment (494 bp) containing the 5S rRNA gene (but not the region containing the ARS cores) hybridized to genomic DNAs from a number of yeast species, including S. cerevisiae, C. tropicalis, C. pseudotropicalis, C. parapsilosis, C. kruseii, C. (Torulopsis) glabrata and Neurospora crassa. The 709-bp ARS element (but not the 5S rRNA gene) was necessary for high-frequency transformation and autonomous plasmid replication in both S. cerevisiae and C. albicans.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00261723
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  • 7
    Digitale Medien
    Digitale Medien
    The curing reaction of an epoxide resin with 2-ethyl-4-methylimidazole, a calorimetric study of the kinetics of formation of epoxide-imidazole adducts (1975)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 176 (1975), S. 919-930 
    Details
    ISSN: 0025-116X
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Beschreibung / Inhaltsverzeichnis: Die Kinetik der Bildung von 1:1 und 2:1-Addukten (bezogen auf die Äquivalente Epoxid) von 2,3-Epoxipropyloxybenzol (2) und 2,2-Bis[p-(2,3-epoxypropyloxy)phenyl]-propan (1) mit 2-Äthyl-4-methylimidazol (3) wurde mit Hilfe der Differentialkalorimetrie (DSC) untersucht. Ein Vergleich isothermer und dynamischer Werte des 1:1-Systems 2/3 zeigte, daß die dynamische Geschwindigkeit nicht einfach mit der isothermen Geschwindigkeit gleichgesetzt werden kann, wohingegen die wahre dynamische Geschwindigkeitsgleichung dα/dt = (∂α/∂tT)+(∂α/∂Tt) (dT/dt), gute Übereinstimmung mit den isothermen Werten ergab. Die dynamischen DSC-Messungen deuten darauf hin, daß alle Reaktionen angenähert nach der 1. Ordnung verlaufen und ähnliche Werte für die Aktivierungsenergie (E = 81-93 kJ/mol) und den Frequenzfaktor (log A ≈ 9-10) ergeben. Die Ergebnisse stützen den Vorschlag, daß die Adduktbildung wahrscheinlich die erste Stufe der durch 3 katalysierten Harzvernetzung darstellt.
    Notizen: The kinetics of formation of the 1:1 and 2:1 adducts of (in terms of equivalents of epoxide) 2,3-epoxypropyloxybenzene (2) and 2,2-bis[p-(2,3-epoxypropyloxy)phenyl]-propane (1) with 2-ethyl-4-methylimidazole (3) have been investigated by differential scanning calorimetry (DSC). A comparison of isothermal and dynamic data for the 1:1 system 2/3 showed that the dynamic rate cannot simply be equated to the isothermal rate, but the true dynamic rate equation, dα/dt = (∂α/∂tT)+(∂α/∂Tt) (dT/dt), gave good agreement with the isothermal data. The dynamic DSC measurements indicate that all of the reactions approximate to first order kinetics and have similar values for activation energy (E = 81-93 kJ/mol) and pre-exponential factor (log A ≈ 9-10). The results support the suggestion that adduct formation is likely to be the first stage of the resin curing reaction catalyzed by 3.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/macp.1975.021760408
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  • 8
    Digitale Medien
    Digitale Medien
    Development of in situ blends of poly(p-oxybenzoate-co-p-phenyleneisophthalate) with other thermotropic liquid crystalline polymers (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 53 (1994), S. 575-586 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: A new in situ polymerization method is discussed wherein monomers of the major component are polymerized in the presence of a preformed thermotropic liquid crystalline polymer (LCP). Poly(p-oxybenzoate-co-p-phenyleneisophthalate) (HIQ) was chosen for this study. HIQ is well known to have a narrow process window and to be difficult to process reproducibly, both drawbacks for a commercial product. An in situ polymerization of the HIQ monomers in the presence of a fully polymerized LCP, such as poly(4-oxybenzoate-co-6-oxy-2-naphthoate) (CO) results in the “CO-HIQ” in situ blend. The in situ blend exhibited reduced biphasic morphology and was more homogeneous than was HIQ. The glass fiber-filled CO-HIQ resin also featured improved melt flow and more reproducible mechanical properties than those of HIQ. The in situ blends were characterized by optical microscopy, 1H-NMR, and melt rheology. The effect of the LCP level on the morphology of the in situ blend is discussed. © 1994 John Wiley & Sons, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1994.070530510
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  • 9
    Digitale Medien
    Digitale Medien
    An investigation of the effects of the molecular weight distribution on the sensitivity of positive electron resists (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 20 (1976), S. 2367-2375 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: The molecular weight distribution of two grades of poly(methyl methacrylate) was determined, and the solubility of that polymer in methyl ethyl ketone-2-propanol mixtures was studied. The effects of the molecular weight distribution of the polymer and the strength of the developing solvent on electron resist sensitivity were studied experimentally and compared with a computer model of the electron exposure process. The molecular weight and the molecular weight distribution were found to have a significant effect on the sensitivity of poly(methyl methacrylate) as an electron resist. Increased sensitivity is predicted for higher molecular weight and narrower molecular weight distribution.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1976.070200907
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  • 10
    Digitale Medien
    Digitale Medien
    Mechanical properties of a hydrophilic acrylic polymer (1972)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 6 (1972), S. 15-24 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Acrylic polymers with hydrophilic groups (i.e., ethyl hydroxyl) may be prepared that convert to a soft elastic gel by absorbing water. Such a polymer has been applied to dentistry for use as an elastic lining material for dentures. A commercial product, Hydron, was used in this study. The linear expansion of this material after 520 hr of water immersion was found to be 20.4%. Hardness was found to decrease from 15.7 KHN for the dry polymer to 1.5 KHN at 19% absorbed water content. The swelling pressure was measured and found to reach a maximum value of 157 psi after 204 hr of water immersion. The Young's modulus was found to decrease from a value of 295,300 psi to 290 psi with 19% absorbed water content. Values were determined on intermediate water contents for the properties studied.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820060204
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