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  • 1
    Electronic Resource
    Electronic Resource
    Rapid purification of transfected porcine muscle cells (2000)
    Springer
    Methods in cell science 22 (2000), S. 217-223 
    Details
    ISSN: 1573-0603
    Keywords: Flow cytometry ; Muscle cell ; Porcine ; Transfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A detailed methodology is described for fluorescence-activated cell sorting (FACS) of porcine muscle cells that have been transfected to express green fluorescent protein (GFP). Cells are liberated from porcine skeletal muscle and primary cultures are transfected with DNA encoding GFP. Primary cultures are subjected to immunocytochemistry using a primary muscle-specific monoclonal antibody followed by incubation with a phycoerythrin-conjugated second antibody. Transfected myoblasts aresorted from fibroblasts using forward angle light scatter and ninety degree light scatter, phycoerythrin fluorescence, and GFP fluorescence. These procedures allow for isolation of genetically-engineered porcine muscle cells more rapidly than traditional clonal selection procedures. Consequently, FACS provides porcine myoblast populations that retain the majority of their replicative capacity and are not contaminated with non-myogenic cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009856411749
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  • 2
    Electronic Resource
    Electronic Resource
    Stable resistance in barley to Pyrenophora teres f. teres isolates from the Nordic-Baltic region after increase on standard host genotypes (2000)
    Springer
    Euphytica 113 (2000), S. 71-77 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; net blotch ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Results from tests of a mixture of Finnish net blotch, Pyrenophora teres Drechs. f. teres Smedeg., isolates on a differential series of barley seedlings, comprising 17genotypes, indicated that patterns of infection response (IR)and percentage leaf area damaged (PLAD) were unaffected by differences in seedling size. Variation of the concentration of inoculum between 1,250 conidia ml-1 and 20,000 conidia ml-1 produced similar patterns of IR and PLAD on the differential series. IR and PLAD scored on the second seedling leaf differentiated resistance to P. teres f. teresamong the genotypes better than on the first seedling leaf. Ina second experiment, 120 single-spore P. teres f. teres isolates from Finland, Sweden, Norway, Latvia, Estonia and Ireland were used in tests conducted in the greenhouse to differentiate them in terms of virulence reaction on seedlings of six differential barley genotypes. Each isolate was tested directly following isolation from the leaf material and after having passaged each through barley cvs. Arve or Pohto, to produce 360 isolates in total. Virulence of the isolates differed significantly on the members of the differential series, but differences associated with country of origin and passaging, and interactions, were small. It is concluded that little variation between virulence of P. teres f. teres isolates is evident over a large geographic area, incorporating Nordic and Baltic countries, and Ireland. Barley genotype response to P. teres f. teres appeared to be of more significance than relative virulence of the pathogen isolates. This could simplify breeding barley for improved resistance to this phytopathogen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1003912825455
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  • 3
    Electronic Resource
    Electronic Resource
    Residual stress prediction and determination in 7010 aluminum alloy forgings (2000)
    Springer
    Experimental mechanics 40 (2000), S. 75-82 
    Details
    ISSN: 1741-2765
    Keywords: residual stress ; aluminum alloy ; forging ; solution heat treatment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Precipitation-hardened aluminum alloys gain their high strength through heat treatment involving a severe quenching operation, which can have the adverse effect of introducing residual stresses. The finite element code ABAQUS is used to simulate the quenching of aluminum alloy 7010 in an attempt to predict the residual stress distribution that develops in simple shapes. The rate of heat transfer from the material is determined using the finite element method to predict the heat transfer coefficient from surface cooling curves achieved experimentally. The flow stress of the material is assumed to be strain rate dependent and to behave in a perfectly plastic manner. The predicted residual stress magnitudes and directions are compared to values determined using the holedrilling strain gage method and the X-ray diffraction technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02327551
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