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  • 16S rRNA phylogeny  (3)
  • Methylococcus capsulatus (Bath)  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Methylosulfonomonas methylovora gen. nov., sp. nov., and Marinosulfonomonas methylotropha gen. nov., sp. nov.: novel methylotrophs able to grow on methanesulfonic acid (1997)
    Springer
    Archives of microbiology 167 (1997), S. 46-53 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsMethylosulfonomonas methylovora ; Marinosulfonomonas methylotropha ; Methanesulfonic ; acid ; 16S rRNA phylogeny ; 16S rDNA sequences ; Proteobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two novel genera of restricted facultative methylotrophs are described; both Methylosulfonomonas and Marinosulfonomonas are unique in being able to grow on methanesulfonic acid as their sole source of carbon and energy. Five identical strains of Methylosulfonomonas were isolated from diverse soil samples in England and were shown to differ in their morphology, physiology, DNA base composition, molecular genetics, and 16S rDNA sequences from the two marine strains of Marinosulfonomonas, which were isolated from British coastal waters. The marine strains were almost indistinguishable from each other and are considered to be strains of one species. Type species of each genus have been identified and named Methylosulfonomonas methylovora (strain M2) and Marinosulfonomonas methylotropha (strain PSCH4). Phylogenetic analysis using 16S rDNA sequencing places both genera in the α-Proteobacteria. Methylosulfonomonas is a discrete lineage within the α-2 subgroup and is not related closely to any other known bacterial genus. The Marinosulfonomonas strains form a monophyletic cluster in the α-3 subgroup of the Proteobacteria with Roseobacter spp. and some other partially characterized marine bacteria, but they are distinct from these at the genus level. This work shows that the isolation of bacteria with a unique biochemical character, the ability to grow on methanesulfonic acid as energy and carbon substrate, has resulted in the identification of two novel genera of methylotrophs that are unrelated to any other extant methylotroph genera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050415
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  • 2
    Electronic Resource
    Electronic Resource
    Taxonomic relationships ofThiobacillus halophilus, T. aquaesulis, and other species ofThiobacillus, as determined using 16S rDNA sequencing (1996)
    Springer
    Archives of microbiology 166 (1996), S. 394-398 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus taxonomy ; Thiobacillus aquaesulis ; Thiobacillus halophilus ; 16S rRNA phylogeny ; 16S rDNA sequences ; Proteobacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Total base sequences of the 16S rRNA genes ofThiobacillus halophilus andThiobacillus aquaesulis show that these bacteria fall into the gamma- and beta-subdivisions, respectively of the Proteobacteria. The closest relative ofT. halophilus isThiobacillus hydrothermalis (with 98.7% similarity), and the closest relative ofT. aquaesulis isThiobacillus thioparus (93.2% similarity). Physiological properties and mol% G+C content of their DNA serve to confirm that these four organisms are all distinct species. It is reiterated that the species currently assigned to the genusThiobacillus are clearly so diverse that they need reclassification into several genera. The type species,T. thioparus, is unequivocally placed in the beta-subdivision of the Proteobacteria, thus requiring that the use of the genus nameThiobacillus be restricted to the chemolithoautotrophic species falling into that group.T. aquaesulis andT. thioparus may thus be regarded as true species ofThiobacillus. The relatively large number of obligately chemolithoautotrophicThiobacillus species falling in the gamma-subdivision of the Proteobacteria need further study in order to assess the case for reclassification into one or more new or different genera.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01682985
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  • 3
    Electronic Resource
    Electronic Resource
    Autotrophic growth on carbon disulfide is a property of novel strains of Paracoccus denitrificans (1997)
    Springer
    Archives of microbiology 168 (1997), S. 225-236 
    Details
    ISSN: 1432-072X
    Keywords: Key words Paracoccus denitrificans ; Paracoccus ; versutus ; CS2 oxidation ; CS2 oxygenase ; Autotrophy ; 16S rRNA phylogeny ; Plasmids ; Megaplasmids ; Murein ; Gas chromatography ; SDS-PAGE profiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three distinct strains (KL1, KS1, and KS2) of facultatively chemolitho-autotrophic bacteria able to use carbon disulfide or carbonyl sulfide as sole energy substrates were identified as novel strains of Paracoccus denitrificans. Evidence for their identity as biovars of P. denitrificans and as close relatives of Paracoccus versutus is based on their DNA composition, total sequencing of the genes for their 16S rRNA, muropeptide profiles, amino acid composition of peptidoglycan, kinetics of murein degradation by lysozyme, possession of large plasmids (91–98 kb) and megaplasmids (〉 450 kb), and plasmid transfer between the strains and with P. denitrificans and P. versutus. No functions have been identified for the 91- to 98-kb plasmids of strains KL1 and KS2, but curing strain KL1 of its plasmid did not affect growth on carbon disulfide, thiosulfate or succinate. Emendation of the formal description of Paracoccus denitrificans is presented. Autotrophic growth on carbon disulfide and thiosulfate was confirmed by 14CO2 fixation. Evidence is presented for initiation of carbon disulfide oxidation by an NADH-dependent oxygenase. Cell-free extracts catalyzed (1) NADH-stimulated uptake of oxygen in the presence of carbon disulfide, and (2) carbon-disulfide-stimulated oxidation of NADH. The activity was not sedimented at 50,000 ×g. Intermediates in aerobic carbon disulfide metabolism were shown by GC and GC/MS to include carbonyl sulfide and hydrogen sulfide, but anaerobic production of COS and H2S from carbon disulfide did not occur. SDS-PAGE of cell-free extracts showed polypeptides that were unique to growth on carbon disulfide, common to carbon disulfide and carbonyl sulfide, or found after growth on carbon disulfide, carbonyl sulfide or thiosulfate. The possible identity of these as proteins involved in sulfur compound metabolism is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050492
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular analysis of methane monooxygenase from Methylococcus capsulatus (Bath) (1989)
    Springer
    Archives of microbiology 152 (1989), S. 154-159 
    Details
    ISSN: 1432-072X
    Keywords: Methanotroph ; Methane oxidation ; Methane monooxygenase ; Oligonucleotide probing ; Subunit genes ; Methylococcus capsulatus (Bath)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Methane monooxygenase (MMO) is the enzyme responsible for the conversion of methane to methanol in methanotrophic bacteria. In addition, this enzyme complex oxidizes a wide range of aliphatic and aromatic compounds in a number of potentially useful biotransformations. In this study, we have used biochemical data obtained from purification and characterization of the soluble MMO from Methylococcus capsulatus (Bath), to identify structural genes encoding this enzyme by oligonucleotide probing. The genes encoding the β and γ subunits of MMO were found to be chromosomally located and were linked in this organism. We report here on the analysis of a recombinant plasmid containing 12 kilobases of Methylococcus DNA and provide the first evidence for the localization and linkage of genes encoding the methane monooxygenase enzyme complex. DNA sequence analysis suggests that the primary structures of the β and γ subunit of MMO are completely novel and the complete sequence of these genes is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00456094
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  • 5
    Electronic Resource
    Electronic Resource
    Molecular genetics of methane oxidation (1994)
    Springer
    Biodegradation 5 (1994), S. 145-159 
    Details
    ISSN: 1572-9729
    Keywords: methane oxidation ; methane monooxygenase gene ; Methylococcus capsulatus (Bath) ; Methylosinus trichosporium OB3b
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Biological methane oxidation is carried out by methanotrophs, bacteria that utilize methane as their sole carbon and energy source. The enzyme they contain that is responsible for methane oxidation is methane monooxygenase, the most well studied being the soluble methane monooxygenase enzyme complexes fromMethylococcus capsulatus (Bath) andMethylosinus trichosporium OB3b. In both organisms, the genes encoding soluble methane monooxygenase have been found to be clustered on the chromosome in the ordermmoX, mmoY, mmoB, mmoZ, orfY andmmoC. These genes encode the α and β subunits of Protein A, Protein B, the γ subunit of Protein A, a protein of unknown function and Protein C respectively of the soluble methane monooxygenase complex. The complete DNA sequences of both gene clusters have been determined and they show considerable homology. Expression of soluble methane monooxygenase genes occurs under growth conditions where the copper-to-biomass ratio is low. Transcriptional regulation of the gene cluster fromMethylosinus occurred at an RpoN-like promoter, 5′ of themmoX gene.mmoB andmmoC ofMethylococcus have been expressed inE. coli and the proteins obtained were functionally active. Soluble methane monooxygenase mutants have been constructed by marker-exchange mutagenesis. They were found to be more stable than those generated using the suicide substrate dichloromethane. Soluble methane monooxygenase probes have been used to detect both methane monooxygenase gene-specific DNA and methanotrophs in natural environmental samples.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00696456
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