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  • Male sterility  (3)
  • Aerobic growth
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 167 (1997), S. 167-171 
    ISSN: 1432-072X
    Keywords: Key wordsZymomonas mobilis ; Biomass yield ; Aerobic growth ; Anaerobic growth ; Ethanol formation ; Pasteur effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Elevated values of molar growth yield (Yx/s = 14–26 g mol–1) were obtained during exponential growth (μ 〉 0.4 h–1) of Zymomonas mobilis ATCC 29191 by using reduced concentrations of glucose (6.25–100 mM) and increased oxygen supply (E h 〉 300 mV) in the growth medium, as compared to the Yx/s of anaerobic exponential growth (8–10 g mol–1). Aerobically grown cells showed an increased maximum growth rate (μmax), and a reduced specific glucose consumption rate (qs), and specific ethanol formation rate (qp), thus demonstrating a more pronounced energy-coupling growth under oxic conditions. These results can be neither explained by the concept of a solely operating Entner-Doudoroff pathway as an ATP source in aerobically growing cultures of Z. mobilis nor considered to be consistent with existing data on the lack of the Pasteur effect in this bacterium. Therefore, the results rather give evidence for the essential contribution of aerobic ATP generation under the reported conditions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 10 (1997), S. 22-26 
    ISSN: 1432-2145
    Keywords: Key words Maize ; Pollen ; Male sterility ; Gametophytic mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to dissect the complex genetic system that controls pollen development, we have undertaken a program of transposon insertion mutagenesis, with the purpose of producing mutations in gametophytically acting genes that are important for this process. The present work reports the developmental cytology of one of the mutants isolated, gaMS-2 (gametophytic male sterile-2). A peculiar feature of the mutant grains was lack of differentiation between the vegetative and the generative nuclei, leading to alteration in number, conformation and placement of nuclei. At anthesis, the grains carrying the mutant allele are about 40% of the normal grain size, contain a very reduced amount of starch and exhibit various nuclear abnormalities.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 9 (1996), S. 216-220 
    ISSN: 1432-2145
    Keywords: Key words Maize pollen ; Male sterility ; Microsporogenesis ; Gametophytic gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization of gametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis. gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, non-functional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Sexual plant reproduction 9 (1996), S. 216-220 
    ISSN: 1432-2145
    Keywords: Maize pollen ; Male sterility ; Microsporogenesis ; Gametophytic gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several pollen-specific genes from different species have been isolated and characterized at the molecular level, but the precise role of most of them is unknown. Mutant analysis represents a direct approach to uncovering gene function, but the paucity of available mutants affecting pollen development and/or function and the poor characterization of the known mutants have so far limited the exploitation of this approach. Here we present the cytological characterization ofgametophytic male sterile-1 (gaMS-1), a maize mutant that we identified in a program of transposon insertion mutagenesis for the production of mutations in gametophytically acting genes involved in microsporogenesis.gaMS-1 is expressed during or immediately after the first microspore division and leads to the production of immature, nonfunctional pollen grains. The mutation appears to affect the events leading to the developmental switch that follows the first microspore mitosis.
    Type of Medium: Electronic Resource
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