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  • 1
    ISSN: 1432-0878
    Keywords: Labial mucosa ; Buccal mucosa ; Macaca fascicularis ; Stereology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Lippen- und Wangenmukosa wurde an 7 Affen (6 M. fascicularis, 1 M. mulatta; 2,4±0,6 kg schwer) morphologisch und quantitativ untersucht. Nach einer Perfusionsfixation wurden Ober- und Unterlippen sowie ganze Wangen herauspräpariert und für Untersuchungen im Licht-, Raster und Transmissionselektronenmikroskop vorbereitet. Bestehende Programme (HISTOMEP, MUMANA II) und morphometrische Standardmethoden wurden verwendet, um lichtmikroskopisch die Epitheldicke, die Breite der kombinierten Lamina propria/Submukosa und die volumetrische Zusammensetzung der drüsenhaltigen Abschnitte der Lippen- und Wangenschleimhaut zu ermitteln. Das Wangenepithel war mehr als zweifach dicker als das Lippenepithel (0,46±0,04 und 0,21±0,02mm); die Epitheldicke war unabhängig von Geschlecht und Topographie. Die kombinierte Lamina propria/ Submukosa war im Wangenbereich 1,32±0,19, im Lippenbereich 1,50± 0,26 mm breit. Die Hauptelemente der Lippen- und Wangenschleimhaut sind Drüsen- und Bindegewebe sowie mit Ausführungsgängen assoziierte Lymphfollikel. In der Lippen-, nicht aber in der Wangenschleimhaut, ist das Volumen der die Drüsenacini umlagernden Plasmazellen positiv korreliert mit der Menge der Lymphfollikel an den benachbarten Ausführungsgängen. Es wird vermutet, daß das Ausführungsgang/Lymphfollikel-System zur lokalen Erkennung von Antigenen dienen könnte.
    Notes: Summary In seven monkeys (6 Macaca fascicularis, 1 M. mulatta; 2.4±0.6 kg in weight) the labial and buccal mucosae were studied morphologically and quantitatively. Following fixation by perfusion, the upper and lower lips and entire cheeks were dissected free and processed for light-, scanning and transmission electron microscopy. Established programs (HISTOMEP, MUMANA II) and appropriate morphometric techniques were used to estimate, at the light-microscopic level, the epithelial thickness, the width of the combined lamina propria/submucosa, and the volumetric composition of the gland-containing portions of lip and cheek mucosae. The cheek epithelium was more than twofold thicker than the lip epithelium, on the average 0.46±0.04 and 0.21±0.02 mm, respectively, with no differences related to sex or topographical sites. The combined lamina propria/submuscosa was 1.32 ±0.19 and 1.50±0.26 mm in width in cheeks and lips, respectively. The main mucosal constituents at both sites were glandular and connective tissue, and lymph follicles associated with secretory ducts. In lips, the volume of plasma cells around gland acini correlated positively with the amount of lymphoid tissue present around topographically related ducts. It is suggested that the duct/ lymph follicle assembly may serve as a local antigen-recognition system.
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  • 2
    ISSN: 1432-0878
    Keywords: Oral mucosa ; Age-dependent changes ; Lymphoid tissue ; Macaca fascicularis ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In three mature monkeys (Macaca fascicularis; 3.5±0.3 kg in weight), the labial, buccal and soft-palate mucosae were examined morphologically and stereologically. Using fixation by perfusion, standardized methods of tissue preparation and morphometric analysis at the light-microscopic level, the gross dimensions (i.e., epithelial thickness, width of combined lamina propria/submucosa) and the volumetric composition of the oral mucosae were estimated and compared with those of young animals examined previously. The data show (1) an age-related decline in the volume and prevalence of organized lymphoid tissue (i.e., lymphoid follicles associated with secretory ducts), (2) a stable plasma-cell density in the interglandular connective tissue, and (3) an increase of glandular tissue in mature versus young animals. It is suggested that the lymphoid follicles associated with secretory ducts, providing for plasma-cell generation, mirror the tonsillar lymphoid tissue declining after puberty.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 240 (1985), S. 223-232 
    ISSN: 1432-0878
    Keywords: Oral mucosa, simian ; Local mucosal immunity ; Minor salivary glands ; Duct/lymphoid follicle assemblies ; Ultrastructure ; Macaca fascicularis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous light-microscopic and ultra-immunohistochemical tracer studies revealed the existence of an independent local immune response of the simian oral mucosa. This local response is attributed to the presence of minor salivary gland (MSG) duct-related lymphoid tissue. Semithin sections from a total of 263 Epon-embedded tissue blocks from the labial and buccal mucosae of seven monkeys, Macaca fascicularis, were analysed light-microscopically, and 10 suitable MSG duct/follicle assemblies were investigated ultrastructurally. These duct/follicle assemblies include follicular and parafollicular compartments with distinct fine-structural elements. The follicular area or germinal centre contains numerous small and large lymphoid cells, mitotic figures, plasmablasts, macrophages, and cells resembling the follicular dendritic cells with distinct desmosomal junctions. The parafollicular area, which includes the heavily infiltrated duct wall, contains numerous small lymphocytes, T-lymphoblasts, plasma cells and reticular cells resembling fibroblasts. A distinct feature of this compartment is the presence of high endothelial venules (HEV). The presence of HEV and numerous blast cells, resembling blast-forming T-lymphocytes activated in vitro, in a specific area of the duct/follicle assembly strongly suggests that this area is structurally and physiologically identical to the thymus-dependent area of other lymphoid tissues. In other words, the duct/follicle assemblies of simian MSG contain the various specific fine-structural elements that are suitable for antigen recognition and processing. These elements are distributed in discrete compartments comparable to the B- and T-cell areas of “classical” lymphoid tissue.
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