Publication Date:
2001-02-27
Description:
The mammalian innate immune system retains from Drosophila a family of homologous Toll-like receptors (TLRs) that mediate responses to microbial ligands. Here, we show that TLR2 activation leads to killing of intracellular Mycobacterium tuberculosis in both mouse and human macrophages, through distinct mechanisms. In mouse macrophages, bacterial lipoprotein activation of TLR2 leads to a nitric oxide-dependent killing of intracellular tubercle bacilli, but in human monocytes and alveolar macrophages, this pathway was nitric oxide-independent. Thus, mammalian TLRs respond (as Drosophila Toll receptors do) to microbial ligands and also have the ability to activate antimicrobial effector pathways at the site of infection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Thoma-Uszynski, S -- Stenger, S -- Takeuchi, O -- Ochoa, M T -- Engele, M -- Sieling, P A -- Barnes, P F -- Rollinghoff, M -- Bolcskei, P L -- Wagner, M -- Akira, S -- Norgard, M V -- Belisle, J T -- Godowski, P J -- Bloom, B R -- Modlin, R L -- AI 07118/AI/NIAID NIH HHS/ -- AI 22553/AI/NIAID NIH HHS/ -- AI 47868/AI/NIAID NIH HHS/ -- AR 40312/AR/NIAMS NIH HHS/ -- New York, N.Y. -- Science. 2001 Feb 23;291(5508):1544-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Dermatology, Department of Microbiology and Immunology and Molecular Biology Institute, UCLA School of Medicine, Los Angeles, CA 90095, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11222859" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bacterial Proteins/immunology
;
Cell Line
;
Cells, Cultured
;
*Drosophila Proteins
;
Humans
;
Interferon-gamma/immunology/pharmacology
;
Ligands
;
Lipoproteins/*immunology
;
Macrophage Activation
;
Macrophages/immunology/metabolism/*microbiology
;
Macrophages, Alveolar/immunology/metabolism/microbiology
;
Macrophages, Peritoneal/immunology/metabolism/microbiology
;
Membrane Glycoproteins/*metabolism
;
Mice
;
Monocytes/immunology/metabolism/*microbiology
;
Mycobacterium tuberculosis/growth & development/*immunology
;
Nitric Oxide/*metabolism
;
Nitric Oxide Synthase/antagonists & inhibitors/metabolism
;
Nitric Oxide Synthase Type II
;
Receptors, Cell Surface/*metabolism
;
Signal Transduction
;
Toll-Like Receptor 2
;
Toll-Like Receptors
;
Tumor Necrosis Factor-alpha/immunology/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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