ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Modulation of cardiac myocyte phenotype in vitro by the composition and orientation of the extracellular matrix (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 161 (1994), S. 89-105 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cellular phenotype is the result of a dynamic interaction between a cell's intrinsic genetic program and the morphogenetic signals that serve to modulate the extent to which that program is expressed. In the present study we have examined how morphogenetic information might be stored in the extracellular matrix (ECM) and communicated to the neonatal heart cell (NHC) by the cardiac α1β1 integrin molecule. A thin film of type I collagen (T1C) was prepared with a defined orientation. This was achieved by applying T1C to the peripheral edge of a 100 mm culture dish. The T1C was then drawn across the surface of the dish in a continuous stroke with a sterile cell scraper and allowed to polymerize. When NHCs were cultured on this substrate, they spread, as a population, along a common axis in parallel with the gel lattice and expressed an in vivo-like phenotype. Individual NHCs displayed an elongated, rod-like shape and disclosed parallel arrays of myofibrils. These phenotypic characteristics were maintained for at least 4 weeks in primary culture. The evolution of this tissue-like organizational pattern was dependant upon specific interactions between the NHCs and the collagen-based matrix that were mediated by the cardiac α1β1 integrin complex. This conclusion was supported by a variety of expermental results. Altering the tertiary structure of the matrix or blocking the extracellular domains of either the cardiac α1, or β1 integrin chain inhibited the expression of the tissue-like pattern of organization. Neither cell-to-cell contact or contractile function were necessary to induce the formation of the rod-like cell shape. However, beating activity was necessary for the assembly of a well-differentiated myofibrillar apparatus. These data suggest that the cardiac α1 β1 integrin complex serves to detect and transduce phenotypic information stored within the tertiary structure of the surrounding matrix. © 1994 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041610112
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Action of phosphatidylcholine in protecting ram sperm from cold shock (1986)
    New York, NY : Wiley-Blackwell
    Gamete Research 15 (1986), S. 43-56 
    Details
    ISSN: 0148-7280
    Keywords: calcium ; ultrastructure ; motility ; respiration ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Rapid cooling (cold shocking) of washed ejaculated ram sperm to 0°C irreversibly reduced motility, tail beat frequency, and respiration and increased the uptake of 45Ca2+. The plasma membranes were removed from the sperm head, and the acrosomes were detached from the nuclei. The plasma membranes of the middle piece were removed, and the mitochondria contained pale and expanded cristae, similar in appearance to ATP-deprived mitochondria in the “condensed” configuration. The presence of 2.0 mg/ml phosphatidylcholine (lecithin) in the medium prevented ultrastructural damage on cold shock, and the motility, tail beat frequency, respiratory rate, and calcium uptake were maintained at levels similar to washed sperm. As the “protective” effect of phosphatidylcholine against cold shock was maintained to a certain extent after rewashing and centrifuging the sperm prior to cold shock, the interaction of phosphatidylcholine with ram sperm membranes may be fairly “tight” and not easily disrupted.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120150106
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Susceptibility of epididymal boar sperm to cold shock and protective action of phosphatidylcholine (1987)
    New York, NY : Wiley-Blackwell
    Gamete Research 17 (1987), S. 355-373 
    Details
    ISSN: 0148-7280
    Keywords: motility ; respiration ; calcium ; ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Rapidly cooling (cold shocking) washed cauda boar sperm irreversibly reduced motility and respiration and greatly increased the uptake of 45Ca2+; the plasma membranes were removed and the acrosomes detached from nuclei. The motility, respiration, and calcium uptake of the less mature caput sperm were largely unaffected; and there was little damage to the ultrastructure. This indicates that boar sperm becomes less resistant to cold shock as they mature in the epididymis.The oxygen uptake, glucose breakdown, and lactic acid production of control caput sperm was less than that of cauda sperm. This suggest that the maturation of sperm in the epididymis of the boar involves an increase in both the glycolytic and oxidative phases of glucose metabolism.The presence of 2.0 mg/ml phosphatidylcholine (lecithin) in the medium prevented ultrastructural damage to cauda sperm on cold shock, and motility and respiration were maintained at levels similar to those of control sperm. Although the presence of phospholipid reduced the large calcium influx following cold shock, it was still greater that that of control sperm.The “protective” effect against cold shock was not maintained after rewashing the sperm free of phosphatidylcholine prior to cold shock, indicating a fairly “loose” interaction of the phospholipid with boar sperm membranes that was easily disrupted.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1120170408
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Newly discovered activities for calcitriol (1,25-dihydroxyvitamin D3): Implications for future pharmacological use (1986)
    New York, NY : Wiley-Blackwell
    BioEssays 4 (1986), S. 65-70 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Recent studies have yielded new insights into the critical importance of adequate vitamin D3 intake and metabolism. Investigations of the actions of 1,25-dihydroxyvitamin D3 (calcitriol) on novel target tissues has revealed that this hormone has functions other than its recognized action in regulating blood calcium and phosphate levels. Reports have characterized calcitriol receptors and activities in organs and tissues as diverse as pancreas, skeletal and heart muscle, blood cells, brain, skin, pituitary, parathyroid, kidney, bone and intestine. These studies suggest functions for calcitriol as varied as the regulation of insulin and prolactin secretion, muscle contractility, immune cell metabolism, melanin synthesis and differentiation of blood cells. This information may ultimately help us to understand the etiologies of several kinds of organ dysfunction and lead to the development of tissue-specific agents for new therapies.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950040206
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Nucleosome Positioning In Vivo and In Vitro (1986)
    New York, NY : Wiley-Blackwell
    BioEssays 4 (1986), S. 172-176 
    Details
    ISSN: 0265-9247
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Nucleosome positioning refers to sequence-specific locations for histones interacting with the nucleic acid. Examples of occurrence of this phenomenon, its possible mechanisms and its significance are presented.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bies.950040408
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    The inhibition of bacterial luminescence by a homologous series of carbamatesThis study has been aided in part by a grant from the American Cancer Society, through the Committee on Growth of the National Research Council, and in part by an Institutional grant from the New Jersey Section of the American Cancer Society to the Department of Biology for fundamental biological research. Some of the carbamates were kindly supply by the Eli Lilly and by the Ciba Pharmaceutical companies. (1951)
    Philadelphia : Wiley-Blackwell
    Journal of Cellular and Comparative Physiology 37 (1951), S. 1-13 
    Details
    ISSN: 0095-9898
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1030370102
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Requirement for glucose during in vitro culture of sheep preimplantation embryos (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 31 (1992), S. 253-257 
    Details
    ISSN: 1040-452X
    Keywords: Glucose ; Pyruvate ; Lactate ; Embryo culture ; Sheep ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Glucose utilization by sheep embryos was examined in 8-cell (N = 36) and blastocyst (N = 36) stages, by measuring conversion of [5-3H]glucose to 3H2O. Fifty percent glucose utilization occurred at 0.79 ± 0.69 mM for 8-cell embryos and ∼0.06 ± 0.15 mM for blastocysts. Development of 1- and 2-cell sheep embryos (N = 264) was examined under different glucose concentrations (0, 1.5, 3, or 6 mM) and in the presence or absence of 0.33 mM pyruvate and 3.3 mM lactate (PL). Overall, the presence of glucose was detrimental (P 〈 0.001) to embryonic development. By contrast, the presence of pyruvate and lactate was beneficial (P〈0.001) to development. An interaction was observed between the concentration of glucose and presence or absence of PL (P 〈0.05). An optimum level of glucose occurs at 0-3 mM in the presence of PL (P ±0.1). Development to the blastocyst stage was observed in medium when supplemented with amino acids and albumin alone. Thus, glucose metabolism is not critical for embryonic development, but beneficial at low concentrations. High concentrations can inhibit development, possibly by inhibiting the tricarboxylic acid (TCA) cycle. Sheep embryos may also be using amino acids as an energy source for development.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080310405
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Ultrastructural study of the mature egg of the marine sponge Stelletta grubii (porifera demospongiae) (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 28 (1991), S. 346-350 
    Details
    ISSN: 1040-452X
    Keywords: Reproduction ; Oogenesis ; Yolk ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Stelletta grubii is an oviparous demosponge, which, during its reproductive period from summer to autumn, has small eggs (80-90 μm) dispersed uniformly in the mesohyl. The nucleolated nucleus is surrounded by dictyosomes containing small vesicles, which contribute to form reserve material. Vesicles, numerous food vacuoles, and groups of mitochondria are observed in the granular cytoplasm. Electron-dense yolk inclusions and lipids are found peripherally. The cortical portion of the egg cytoplasm possesses vacuoles with fibrillar contents. The egg forms pseudopodia, which could permit the capture of numerous bacteria present in the surrounding mesohyl. A thick layer of collagen fibrils, including lophocytes, separates the egg from the surrounding sponge mesohyl. Ultrastructural analysis has demonstrated the presence both of cellular components capable of autosynthetic activity (nutrient vesicles) and of phagocytosis mechanisms (pseudopod capture of bacteria) for the storage of nutrients by the egg.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080280406
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    The growth and differentiation of the regenerating spinal cord of the lizard, Anolis carolinensisSupported by grants from the American Cancer Society, the National Institutes of Health and the Multiple Sclerosis Society. (1970)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 131 (1970), S. 131-151 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The present work describes the ultrastructure of the spinal cord in the regenerating tail of the lizard, Anolis. The distal growing region of the tail contains the advancing ependymal tube which is relatively devoid of axons but already contains channels between ependymal cell processes which anticipate their ingrowth. More proximally, fascicles of naked axons having their origin in the stump are present in the ependymal channels. Therefore, the pattern of fiber regeneration in the spinal cord is prescribed by the ependyma and not by the growing axons. Details of the ultrastructure of proximal, intermediate, and distal regions of the regenerate are reported. Particular attention is paid to the structure and differentiation of the ependymal cells and the relation of the ependyma to other glial cells, to nerve fibers, and to meningeal tissues.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051310202
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Architectural and histochemical analysis of the semitendinousus muscle in mice, rats, guinea pigs, and rabbits (1984)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 181 (1984), S. 155-160 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The architectural and histochemical properties of the anatomically distinct compartments of the semitendinosus muscle (ST) of mice, rats, guinea pigs, and rabbits show that the ST is composed of two separate compartments aligned in series - a destal compartment (STd) and a proximal one (STp). The STp is further subdivided into a ventral head (STpv) and a dorsal head (STpd). The muscle fibers were arranged in parallel to the line of muscle pull within each compartment. The STd has the longest and the STpv the shortest fibers in all species. The physiological cross-sectional area and the estimated tetanic tension was greatest in the STd. Based on the staining pattern for myosin ATPase (alkaline preincubation) and an oxidative indicator (NADH or SDH), the STpv has the highest percentage of slow-oxidative (SO) or SO plus fast-oxidative-glycolytic (FOG) fibers of any portion of the muscle. The differences in fiber-type distributions and architectural designs of the separate compartments suggest a specialization of function of the individual compartments.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051810204
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...