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  • 1
    Electronic Resource
    Electronic Resource
    A computer program for the systematic sampling of sections in microscopic morphometry (1985)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 2 (1985), S. 481-487 
    Details
    ISSN: 0741-0581
    Keywords: Morphometry ; Electron microscopy ; Computer program ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A computer program is described that facilitates systematic and unbiased sampling in morphometry. Input data are coordinates of the four corners of a section as displayed in the position indicators of the microscope stage. Outputs are coordinate values in the form of a regular lattice that describes where to place the stage and perform the sampling on the section. In addition, some other data are provided by the program, such as total area of section, length of sides, etc. The program is written in BASIC but can be easily converted to other computer languages.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060020510
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of CHS4 (CAL2), a Gene of Saccharomyces cerevisiae Involved in Chitin Biosynthesis and Allelic to SKT5 and CSD4 (1997)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 13 (1997), S. 795-807 
    Details
    ISSN: 0749-503X
    Keywords: chitin synthase III ; morphogenesis ; Calcofluor resistance ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: We have cloned CHS4, a gene that complements the resistance to Calcofluor of the Saccharomyces cerevisiae cal2 mutant. We show that CHS4 is allelic to the previously described SKT5 and CSD4 genes. CHS4 encodes a 696 amino acids protein with no potential transmembrane domain. chs4-null mutants are resistant to Calcofluor white and exhibit a considerable reduction in cell wall chitin and in chitin synthase III (CSIII) activity. Biochemical characterization of chitin synthase III from these null mutants indicates that the defect is due to a reduced Vmax of the enzyme. This defect can be overcome in vitro by trypsin treatment of the membrane preparations. Chs4p does not act as a transcriptional or translational regulator of CHS3, the gene coding for the catalytic subunit of CSIII activity, and we therefore propose that Chs4p would be an essential component of the CSIII complex, acting as a post-translational regulator of this activity. In addition to the chitin defect, the chs4 mutant shows a severe defect in mating. © 1997 John Wiley & Sons, Ltd.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    A computer program for morphometry of circular and elliptical profiles on the microscope (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 7 (1987), S. 191-193 
    Details
    ISSN: 0741-0581
    Keywords: Morphometry ; Computer program ; Electron microscopy ; Stereology ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: We describe a short computer program, which is written in Pascal language, to measure the diameter of circular and elliptical profiles in sections. Coordinate pairs on the microscope stage, corresponding to two or three points of a profile, are input to obtain its diameter. The program enables one to take measurements directly on the microscope, thereby reducing photographic work and caliper measurement. While the program is largely designed for electron microscopy, it also may be useful for light microscopy morphometry.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060070307
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