Publication Date:
2009-10-10
Description:
Chronic fatigue syndrome (CFS) is a debilitating disease of unknown etiology that is estimated to affect 17 million people worldwide. Studying peripheral blood mononuclear cells (PBMCs) from CFS patients, we identified DNA from a human gammaretrovirus, xenotropic murine leukemia virus-related virus (XMRV), in 68 of 101 patients (67%) as compared to 8 of 218 (3.7%) healthy controls. Cell culture experiments revealed that patient-derived XMRV is infectious and that both cell-associated and cell-free transmission of the virus are possible. Secondary viral infections were established in uninfected primary lymphocytes and indicator cell lines after their exposure to activated PBMCs, B cells, T cells, or plasma derived from CFS patients. These findings raise the possibility that XMRV may be a contributing factor in the pathogenesis of CFS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lombardi, Vincent C -- Ruscetti, Francis W -- Das Gupta, Jaydip -- Pfost, Max A -- Hagen, Kathryn S -- Peterson, Daniel L -- Ruscetti, Sandra K -- Bagni, Rachel K -- Petrow-Sadowski, Cari -- Gold, Bert -- Dean, Michael -- Silverman, Robert H -- Mikovits, Judy A -- CA104943/CA/NCI NIH HHS/ -- HHSN26120080001E/PHS HHS/ -- New York, N.Y. -- Science. 2009 Oct 23;326(5952):585-9. doi: 10.1126/science.1179052. Epub 2009 Oct 8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Whittemore Peterson Institute, Reno, NV 89557, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19815723" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Antibodies, Viral/blood
;
B-Lymphocytes/immunology/virology
;
Base Sequence
;
Cell Line
;
Cell Line, Tumor
;
Coculture Techniques
;
DNA/genetics
;
Fatigue Syndrome, Chronic/*virology
;
Gammaretrovirus/genetics/immunology/*isolation & purification/physiology
;
Gene Products, env/analysis
;
Gene Products, gag/analysis
;
Genome, Viral
;
Humans
;
Leukocytes, Mononuclear/*virology
;
Lymphocyte Activation
;
Male
;
Mice
;
Molecular Sequence Data
;
Prostatic Neoplasms/virology
;
Retroviridae Infections/epidemiology/transmission/*virology
;
T-Lymphocytes/immunology/virology
;
Tumor Virus Infections/epidemiology/transmission/*virology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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