ISSN:
1617-4623
Keywords:
rap recombination adept with plasmids
;
ninG
;
Lambda vector
;
Recombinational library screening
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A phage lambda gene that gives a 100-fold increase in recombinant frequencies for RecABC pathway-mediated, phage-plasmid homologous recombination (Shen and Huang 1986) maps to ninG (orf-204) of lambda. We call this gene rap, for recombination adept with plasmid. A similar determinant exists in Charon 4A and maps in ϕ80-derived sequences, between nin5 and the Rz homology with lambda. The absence of the Rap+ phenotype from certain lambda vectors explains the inefficiency of screening the resulting phage libraries using phage-plasmid homologous recombination. The mapping of rap permits the construction of lambda vectors more suitable for this screening technique.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00325690
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