Summary
A phage lambda gene that gives a 100-fold increase in recombinant frequencies for RecABC pathway-mediated, phage-plasmid homologous recombination (Shen and Huang 1986) maps to ninG (orf-204) of lambda. We call this gene rap, for recombination adept with plasmid. A similar determinant exists in Charon 4A and maps in ϕ80-derived sequences, between nin5 and the Rz homology with lambda. The absence of the Rap+ phenotype from certain lambda vectors explains the inefficiency of screening the resulting phage libraries using phage-plasmid homologous recombination. The mapping of rap permits the construction of lambda vectors more suitable for this screening technique.
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Communicated by G.R. Smith
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Hollifield, W.C., Kaplan, E.N. & Huang, H.V. Efficient recABC-dependent, homologous recombination between coliphage lambda and plasmids requires a phage ninR region gene. Molec Gen Genet 210, 248–255 (1987). https://doi.org/10.1007/BF00325690
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DOI: https://doi.org/10.1007/BF00325690