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  • 1
    Electronic Resource
    Electronic Resource
    Hotspots of homologous recombination (1994)
    Springer
    Cellular and molecular life sciences 50 (1994), S. 234-241 
    Details
    ISSN: 1420-9071
    Keywords: Homologous recombination ; hotspots ; nucleases ; meiosis ; Escherichia coli ; Chi ; Schizosaccharomyces pombe ; M26
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Homologous recombination occurs at higher than average frequency at and near hotspots. Hotspots are special nucleotide sequences recognized by proteins that promote, directly or indirectly, a rate limiting step of recombination. This review focuses on two well-studied examples, the Chi sites of the bacteriumEscherichia coli and the M26 site of the fission yeastSchizosaccharomyces pombe. Chi, 5′ G-C-T-G-G-T-G-G 3′, is recognized by the RecBCD enzyme, which nicks the DNA near Chi and produces a 3′-ended single-stranded DNA ‘tail’; this tail is a potent substrate for homologous pairing by RecA and single-stranded DNA binding proteins. M26, 5′ A-T-G-A-C-G-T 3′, is recognized by a heterodimeric protein and stimulates, by an as-yet-unknown mechanism, meiotic recombination at and near theade6 gene. Additional hotspots in bacteria, fungi, and mammals enhance recombination directly or indirectly via a variety of mechanisms. Although hotspots are widespread among organisms, the biological role of their localized enhancement of recombination remains a matter of speculation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01924006
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  • 2
    Electronic Resource
    Electronic Resource
    Global control of meiotic recombination genes by Schizosaccharomyces pombe rec16 (rep1 ) (1998)
    Springer
    Molecular genetics and genomics 258 (1998), S. 663-670 
    Details
    ISSN: 1617-4623
    Keywords: Key words Meiosis ; Recombination ; Replication ; Transcript induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Schizosaccharomyces pombe rec16-125 mutation reduces meiotic recombination, delays pre-meiotic DNA synthesis, and reduces the accumulation of some but not other rec gene transcripts. To elucidate the function of the Rec16 global meiotic regulator, we cloned and sequenced rec16. The data revealed that rec16 is identical to rep1, which was previously shown to encode a protein with a zinc-finger motif required for pre-meiotic DNA synthesis. Transcripts of rec16 (rep1) were strongly induced and subsequently degraded during meiosis. In a rec16 (rep1) deletion mutant, meiotic induction of the seven rec genes tested, which appear to be directly involved in meiotic recombination, was significantly reduced or essentially abolished. Deletion of 80% of the gene essentially abolished meiotic recombination, whereas strains deleted for approximately one-quarter of the gene, from either end, retained partial activity. The rec16-125 mutation strongly reduced recombination in the intervals tested on chromosomes I and III, a phenotype characteristic of mutations in rec genes, such as rec7, whose expression requires Rec16 (Rep1). These results show that Rec16 (Rep1) does not have the regional specificity of Rec10. We infer that Rec16 (Rep1) is a transcriptional activator that is required for meiotic replication and recombination because it plays a role in the transcriptional induction of the rec and other meiosis-specific genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380050780
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