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  • 1
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Methane ; Clover ; Herb ley ; Cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Nitrous oxide (N2O) emissions and methane (CH4) consumption were quantified following cultivation of two contrasting 4-year-old pastures. A clover sward was ploughed (to 150–200 mm depth) while a mixed herb ley sward was either ploughed (to 150–200 mm depth) or rotovated (to 50 mm depth). Cumulative N2O emissions were significantly greater following ploughing of the clover sward, with 4.01 kg N2O-N ha–1 being emitted in a 48-day period. Emissions following ploughing and rotovating of the ley sward were much less and were not statistically different from each other, with 0.26 and 0.17 kg N2O-N ha–1 being measured, respectively, over a 55-day period. The large difference in cumulative N2O between the clover and ley sites is presumably due to the initially higher soil NO3 – content, greater water filled pore space and lower soil pH at the clover site. Results from a denitrification enzyme assay conducted on soils from both sites showed a strong negative relationship (r=–0.82) between soil pH and the N2O:(N2O+N2) ratio. It is suggested that further research is required to determine if control of soil pH may provide a relatively cheap mitigation option for N2O emissions from these soils. There were no significant differences in CH4 oxidation rates due to sward type or form of cultivation.
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  • 2
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Clover ; Herb ley ; Soil pH ; Allium cepa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  N2O emissions were measured from three contrasting onion (Allium cepa L.) production systems over an 8.5-month period. One system was established on soil where a clover sward had 3 months earlier been ploughed in (ploughed clover site). This production system followed conventional production management practices. The other two systems were established on soil where a mixed herb ley had 3 months earlier been either ploughed or rotovated. These last two production systems followed the guidelines of the International Federation of Organic Agriculture Movements (IFOAM). Cumulative N2O emissions were significantly greater from the ploughed clover site compared to the ploughed ley site (3.8 and 1.6 kg N2O-N ha–1, respectively), while cumulative N2O emissions from the ploughed ley and rotovated ley sites were not significantly different from each other. Emissions from all sites were dominated by episodes of high N2O flux activity following seedbed preparation and drilling, when soil water suction (SWS) was shown to be the rate-controlling variable. The decline in the N2O fluxes after these peak emissions followed clear exponential relationships of the form F=Ae– kt (r≥0.91), where F is the daily flux and A is the y-intercept. First-order decay constants (k) during these periods of declining N2O fluxes (corresponding to half-lives of 2.6–3.0 days) were not significantly different in magnitude from the first-order rate constants that characterised the increasing SWS. Gross differences in cumulative emissions between the clover and ley sites were attributed to the influence of differing soil pHs at the two sites on the N2O:(N2O+N2) ratio in the denitrification products. It also appeared that fertiliser applications to the clover site had both direct and indirect effects on N2O emissions by: (1) enhancing N2O emissions via potential nitrification, (2) increasing the NO3 – supply for enhanced N2O emissions via denitrification, and (3) influencing the N2O:(N2O+N2) ratio by lowering soil pH and increasing NO3 – concentrations. Onion crop yields were greater at the clover site, mainly due to the higher density of planting made possible under a conventional production philosophy. Expressing the yield on the basis of net N2O emissions, 23 t onions kg–1 N2O-N was obtained from the ploughed clover, which was double that obtained for the two systems based on the ley site. However, when the N2O emissions from the cultivation of the soils prior to the sowing of the onions was included, all three systems produced a similar yield per kilogram of N2O-N emitted, averaging 10 t kg–1.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 28 (1998), S. 51-55 
    ISSN: 1432-0789
    Keywords: Key words Nitrous oxide ; Nitrification ; Denitrification ; Soil cores ; Acetylene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Nitrous oxide (N2O) emissions via the nitrification (I nit) and denitrification (I den) pathways were successfully measured with in-field incubation of soil cores in preserving jars at 0 Pa and 5–10 Pa acetylene. From the incubations, fractions of nitrification – N2O over total N2O (I nit / I tot) – and denitrification – N2O over total N2O (I den / I tot) – were obtained. Actual field emissions of N2O via nitrification (F nit) and denitrification (F den) were calculated by multiplying the fractions from the incubation technique with the daily N2O emission (F day) determined with a direct soil cover method. The approach presented here was successful for a whole range of soil moisture conditions in intensive grassland. F nit and F den followed the trends of soil ammonium and soil nitrate.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 65 (1982), S. 19-26 
    ISSN: 1573-5036
    Keywords: Abscisic acid ; Gibberellin ; Rhizobium japonicum ; Roots ; Root nodules ; Soya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The content of endogenous gibberellin (GA)-like substances of roots and root nodules of SOya, and GA production byRhizobium japonicum cultures, were investigated by a combined thin layer chromatographic (TLC)-dwarf pea epicotyl bioassay technique. GAs were more concentrated in root nodules than in the roots, totalling 1.34 and 0.16 nM GA3 equivalents g−1 dry wt. respectively. GA production byR. japonicum cultures was demonstrated (1.00 nM GA3 equivalentsl −1) and comparison of the GA components of plant and bacterial culture medium extracts, suggested that rhizobial GA production may contribute to the nodule GA content. Cis-trans abscisic acid (ABA) was identified in root and nodule extracts by TLC-gas liquid chromatography (GLC), and amounted to 0.18 and 2.21 nM g−1 dry wt. respectively, whereas 0.30 and 4.63 nM ABA equivalents g−1 dry wt. were detected by a TLC-wheat embryo bioassay technique. ABA was not detected in extracts of bacterial cultures.
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  • 5
    ISSN: 1573-5036
    Keywords: Abscisic acid ; Gibberellin ; Polyethylene glycol ; Rhizobium ; Roots ; Nitrogenase ; Nodulation ; Soya ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of 2-day cycles of osmotically induced leaf moisture stress followed by partial recovery on the nodulation and nitrogenase activity of 2 soya cultivars was studied. Fourteen days after plant inoculation (mid-growth stage) the total leaf electrochemical water potential (ψwleaf) of control plants ranged from −0.8 to −1.9 bars, whereas the concentrations of osmoticum (polyethylene glycol 4000) induced ψwleaf values ranging from −1.4 (recovery value) to −3.1 bars (low stress), −1.8 to −4.4 bars (mild stress), and −2.2 to −6.2 bars (medium stress). The low stress treatment reduced nodule numbers and their specific activity in both cultivars, without affecting nodule size or the time required for nodule initiation. Nodule initiation was delayed in both cultivars by the mild and medium stress treatments, the former treatment reducing the number and size of the nodules and such nodules exhibited very low specific activity. The medium stress treatment prevented the further development of nodule initials, which remained inactive throughout the experiment. Such results imply an effect of water stress on the infection process and on nodule morphogenesis. The reduction in nodule numbers observed in water stressed plants was not associated with a reduced number of rhizobia in the rhizoplane nor due to an effect on root growth or root hair formation. At a stage prior to the formation of macroscopic nodule initials, the roots of plants under medium stress (ψwleaf=−5.5 bar)s) had a higher content of abscisic acid (ABA) (4-fold increase) and a lower content of gibberellin (GA)-like substances (21.4% reduction) as compared to control plants (ψwleaf=−1.0 bar). Although the medium stress treatment slightly increased the stomatal resistance of leaves, photosynthetic and transpiration rates were unaffected. Similar alterations of the hormononal balance occurred in the nodulated roots of plants subjected to naturally induced leaf moisture stress. Since the foliar application of ABA (1.92×10−5 M) to unstressed plants inhibited nodulation (45% reduction in nodule numbers), the increased endogenous content of thishormone in the roots of plants under leaf moisture stress may provide some physiological insight into the inhibitory effect of water stress on the nodulation process.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 77 (1984), S. 53-60 
    ISSN: 1573-5036
    Keywords: β-chloroethyltrimethylammonium chloride ; Gibberellins ; Nodulation ; Rhizobium japonicum ; Soya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of exogenous applications of gibberellins (GAs) or the growth retardant β-chloroethyltrimethylammonium chloride (CCC) on root nodule formation and activity (C2H2-reduction) in soya was studied. Daily foliar application of GA3 (2.89×10−6 M) delayed the formation of nodule initials and reduced the numbers mass nodule−1 and specific activity of nodules by 43%, 31% and 47% respectively, without affecting plant growth. Similar effects on nodulation were produced by foliar application of GA4 (3.01×10−5 M) or GA7 (3.03×10−5 M), or by the addition of GA3 (2.89×10−6 M) to the rooting medium. GA effectiveness in reducing nodule numbers was decreased by delaying its application until after the initial infection process had occurred, but the nodules formed were smaller and less active than those of the untreated control plants. The GA effect on nodulation and nodule activity was not associated with alterations in root exudate or due to a direct inhibitory effect of the hormone on the nitrogenase system. When the endogenous root content of GA-like substances was reduced (86% decrease) by foliar application of CCC (6.30×10−5 M), nodule numbers were increased by 56%, but nodule size and total nodule activity were similar to those of control plants. The GA and CCC treatments had no effect on rhizobial growth in liquid culture nor on root colonisation by rhizobia. The results suggest that the endogenous content of root GA may have a regulatory role in both the infection process and in subsequent nodule morphogenesis, thus controlling both the number and effectiveness of the root nodules formed.
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