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  • 1
    Electronic Resource
    Electronic Resource
    A multi-nucleate male-sterile mutant of maize with gametophytic expression (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 22-26 
    Details
    ISSN: 1432-2145
    Keywords: Key words Maize ; Pollen ; Male sterility ; Gametophytic mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to dissect the complex genetic system that controls pollen development, we have undertaken a program of transposon insertion mutagenesis, with the purpose of producing mutations in gametophytically acting genes that are important for this process. The present work reports the developmental cytology of one of the mutants isolated, gaMS-2 (gametophytic male sterile-2). A peculiar feature of the mutant grains was lack of differentiation between the vegetative and the generative nuclei, leading to alteration in number, conformation and placement of nuclei. At anthesis, the grains carrying the mutant allele are about 40% of the normal grain size, contain a very reduced amount of starch and exhibit various nuclear abnormalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050063
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of genetic factors for alachlor tolerance in maize by molecular markers analysis (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 551-555 
    Details
    ISSN: 1617-4623
    Keywords: Key words Maize ; Herbicide ; Molecularmarkers ; Gametophytic selection ; Linkage analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genetic factors controlling tolerance to the herbicide Alachlor in maize were localised by means of two different strategies. In the first approach, backcross (BC) plants, derived from pollen which had been subjected to selective pressure for resistance to the herbicide, were analysed for segregation distortion at 47 RFLP loci and compared to BC plants obtained from non-selected pollen. Preferential transmission of five chromosomal regions where putative QTLs (Quantitative Trait Loci) are localised was revealed in the BC plants from selected pollen. A second approach was based on a classical linkage analysis for segregation of the same set of RFLPs and factors controlling the trait, in a BC population of 210 individuals, by means of regression analysis. This study detected seven significant loci in four genomic regions. Overall, two loci revealed both segregation distortion and association with the expression of the trait, indicating linkage to genes expressed in both gametophytic and sporophytic phase. Three chromosomal regions appeared to carry factors involved in plant tolerance to Alachlor which are not expressed in pollen. Conversely, three loci were linked to factors selectable in pollen, but did not reveal significant association with tolerance in the plant in the segregating populations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173644
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    Paper (German National Licenses)
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