ISSN:
1432-2242
Schlagwort(e):
Key words Haynaldia villosa
;
Triticum aestivum
;
C-banding
;
Genomic in situ hybridization
;
Alien chromosome addition
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract Genomic in situ hybridization (GISH) offers a convenient and effective method for cytological detection, but can not determine the identity of the chromosomes involved. We integrated C-banding with GISH to identify Haynaldia villosa chromosomes in a wheat background. All chromosomes of H. villosa showed C-bands, either in telomeric regions or in both telomeric and centromeric regions, which allowed unequivocal identification of each H. villosa chromosome. The seven pairs of H. villosa chromosomes were differentiated as 1–7 according to their characteristic C-bands. Using a sequential C-banding and GISH technique, we have analyzed somatic cells of F3 plants from the amphiploid Triticum aestivum-H. villosaבYangmai 158’ hybrids. Three plants (94009/5-4, 94009/5-8 and 94009/5-9) were shown to contain H. villosa chromosome(s). 94009/5-4 (2n=45) had three H. villosa chromosomes (2, 3 and 4); 94009/5-8 (2n=45) possessed one chromosome 4 and a pair of chromosome 5, and 94009/5-9 (2n=43) was found to have one chromosome 6 of H. villosa. The combination of GISH with C-banding described here provides a direct comparison of the cytological and molecular landmarks. Such a technique is particularly useful for identifying and localizing alien chromatin and DNA sequences in plants.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/s001220050101
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