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  • 1
    ISSN: 1432-2242
    Keywords: Key words Haynaldia villosa  ;  Triticum aestivum  ; C-banding  ;  Genomic in situ hybridization  ;  Alien chromosome addition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Genomic in situ hybridization (GISH) offers a convenient and effective method for cytological detection, but can not determine the identity of the chromosomes involved. We integrated C-banding with GISH to identify Haynaldia villosa chromosomes in a wheat background. All chromosomes of H. villosa showed C-bands, either in telomeric regions or in both telomeric and centromeric regions, which allowed unequivocal identification of each H. villosa chromosome. The seven pairs of H. villosa chromosomes were differentiated as 1–7 according to their characteristic C-bands. Using a sequential C-banding and GISH technique, we have analyzed somatic cells of F3 plants from the amphiploid Triticum aestivum-H. villosaבYangmai 158’ hybrids. Three plants (94009/5-4, 94009/5-8 and 94009/5-9) were shown to contain H. villosa chromosome(s). 94009/5-4 (2n=45) had three H. villosa chromosomes (2, 3 and 4); 94009/5-8 (2n=45) possessed one chromosome 4 and a pair of chromosome 5, and 94009/5-9 (2n=43) was found to have one chromosome 6 of H. villosa. The combination of GISH with C-banding described here provides a direct comparison of the cytological and molecular landmarks. Such a technique is particularly useful for identifying and localizing alien chromatin and DNA sequences in plants.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Haynaldia villosa ; Triticum aestivum ; C-banding ; Genomic in situ hybridization ; Alien chromosome addition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genomic in situ hybridization (GISH) offers a convenient and effective method for cytological detection, but can not determine the identity of the chromosomes involved. We integrated C-banding with GISH to identify Haynaldia villosa chromosomes in a wheat background. All chromosomes of H. villosa showed C-bands, either in telomeric regions or in both telomeric and centromeric regions, which allowed unequivocal identification of each H. villosa chromosome. The seven pairs of H. villosa chromosomes were differentiated as 1–7 according to their characteristic C-bands. Using a sequential C-banding and GISH technique, we have analyzed somatic cells of F3 plants from the amphiploid Triticum aestivum-H. villosa x ‘Yangmai 158’ hybrids. Three plants (94009/5-4,94009/5-8 and 94009/5-9) were shown to contain H. villosa chromosome(s). 94009/5-4 (2n = 45) had three H. villosa chromosomes (2, 3 and 4); 94009/5-8 (2n = 45) possessed one chromosome 4 and a pair of chromosome 5, and 94009/5-9 (2n = 43) was found to have one chromosome 6 of H. villosa. The combination of GISH with C-banding described here provides a direct comparison of the cytological and molecular landmarks. Such a technique is particularly useful for identifying and localizing alien chromatin and DNA sequences in plants.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2016-01-01
    Description: The spatial mapping of losses attributable to such disasters is now well established as a means of describing the spatial patterns of disaster risk, and it has been shown to be suitable for many types of major meteorological disasters. However, few studies have been carried out by developing a regression model to estimate the effects of the spatial distribution of meteorological factors on losses associated with meteorological disasters. In this study, the proposed approach is capable of the following: (a) estimating the spatial distributions of seven meteorological factors using Bayesian maximum entropy, (b) identifying the four mapping methods used in this research with the best performance based on the cross validation, and (c) establishing a fitted model between the PLS components and disaster losses information using partial least squares regression within a specific research area. The results showed the following: (a) best mapping results were produced by multivariate Bayesian maximum entropy with probabilistic soft data; (b) the regression model using three PLS components, extracted from seven meteorological factors by PLS method, was the most predictive by means ofPRESS/SStest; (c) northern Hunan Province sustains the most damage, and southeastern Gansu Province and western Guizhou Province sustained the least.
    Print ISSN: 1687-9309
    Electronic ISSN: 1687-9317
    Topics: Geosciences , Physics
    Published by Hindawi
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