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  • Cloning vectors  (1)
  • Iso-1-cytochrome c  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Regulation of transcription of the Saccharomyces cerevisiae CYC1 gene: Identification of a DNA region involved in heme control (1984)
    Springer
    Current genetics 8 (1984), S. 45-48 
    Details
    ISSN: 1432-0983
    Keywords: Iso-1-cytochrome c ; Saccharomyces cerevisiae ; Heme ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Saccharomyces cerevisiae mutant (hem1 cycl-1) was transformed with plasmids bearing a chromosomal centromer (CEN3) and a 2 μm DNA replication origin. In one of the plasmids a functional CYC1 gene was present, in a second plasmid an XhoI fragment located between bases -245 and -678 upstream from the translation initiation codon had been deleted, in a third plasmid this region had been inverted. Results of hybridization experiments carried out with mRNA isolated from heme-deficient and heme-containing transformants indicated that heme controls transcription of the CYC1 gene and that DNA sequences located within the upstream XhoI fragment are involved in activation of the gene by heme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00405431
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  • 2
    Electronic Resource
    Electronic Resource
    Construction of a yeast plasmid cloning vector with high stability inSaccharomyces cerevisiae strains deficient in 2 µmDNA (1984)
    Springer
    Monatshefte für Chemie 115 (1984), S. 1229-1235 
    Details
    ISSN: 1434-4475
    Keywords: Cloning vectors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung PlasmidpCP1 wurde ausgehend von klonierterSaccharomyces cerevisiae-2 µmDNA und PlasmidpJDB207 konstruiert. Der Vektor enthält die vollständige, imFLP-Gen unterbrocheneB-Form der 2 µmDNA, vomEscherichia coli-Plasmidp AT153 abgeleiteteDNA und eine wenig aktive Variante desS. cerevisiae-LEU2-Gens. Der neue Vektor weist unter nichtselektiven Wachstumsbedingungen incir +- undcir 0-Stämmen eine niedrige Verlustrate auf und ist incir 0-Stämmen stabil gegen Umlagerungen. Seine Verwendbarkeit für die Umwandlung voncir +-Stämmen incir 0-Stämme durch Verdrängung endogener 2 µm-DNA wurde nachgewiesen.
    Notes: Abstract PlasmidpCP1 was constructed from cloned 2 µmDNA ofSaccharomyces cerevisiae and from plasmidpJDB207. VectorpCP1 contains the completeB form of 2 µmDNA interrupted in theFLP gene, together withDNA derived from theEscherichia coli plasmidpAT153 and a low expression variant of theS. cerevisiae LEU2 gene. The new vector is lost at a low frequency from yeastcir + orcir 0 strains under non-selective growth conditions and is stable against rearrangements incir 0 strains. Its usefulness for curingcir + strains from endogenous 2 µmDNA and for their conversion tocir 0 strains was demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00809354
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    Paper (German National Licenses)
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