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  • Cell & Developmental Biology  (271)
  • INSTRUMENTATION AND PHOTOGRAPHY  (270)
  • 1980-1984  (521)
  • 1935-1939  (20)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Gamete Research 4 (1981), S. 203-217 
    ISSN: 0148-7280
    Keywords: α-chlorohydrin ; antifertility agent ; ram ; sperm metabolism ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The effects of the male antifertility agent, α-chlorohydrin, six of its derivatives, and glycidol were studied on the metabolism of washed ram spermatozoa in vitro with fructose as substrate. The α-chlorohydrin derivatives were the amino, the phosphorylated, and four glycol-bridge (ketal) compounds. All compounds except glycidol, in a concentration between 0.1 and 100 mM, reduced the aerobic glycolsis and/or oxidation of fructose. However, there was not a high correlation between the ability of these compounds to inhibit the metabolism of ram spermatozoa in vitro and their antifertility activity when administered to male rats. Other factors are clearly involved in their antifertility activity, eg, the concentration of the compounds in the epididymis and their conversion of either more or less spermicidal compounds in the body.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 112 (1982), S. 10-18 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Intermediate subviral particles (ISVP) derived from reovirus represent a simple model system for the switch-on of transcriptase function. In such particles the endogenous transcriptase is present in a switched-off form, one step removed from the switched-on state. Switch-on of transcriptase function is an active process in this system and can be triggered by K+ ions. A variety of agents which affect gene expression in cells were tested for an effect on switch-on in ISVP. Marked effects on switch-on in ISVP were observed with a diverse group of test agents, including DMSO and other solvents, BUdR, TdR, caffeine, theophylline, and temperature. The correlation in response between ISVP and cells suggests that the ISVP system may be useful as a model for studying the biochemical mechanisms underlying the perturbative effects of such agents on gene expression in cells.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0148-7280
    Keywords: spermatozoa ; flow cytometry ; DNA staining ; nuclear morphology ; ultrastructure ; mammals ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The morphological and ultrastructural changes that occur during preparation of porcine, bovine, and murine spermatozoa for flow cytometric quantification of the relative DNA content of the X- and Y-chromosome-bearing sperm populations were examined. Ejaculated spermatozoa from the boar and bull were washed using a series of dimethyl sulfoxide (DMSO) solutions prior to fixation, whereas the epididymal mouse spermatozoa were washed only in phosphate-buffered saline (PBS). Spermatozoa from all three species were then fixed in ethanol and processed for fluorochrome staining by a treatment regimen consisting of sulfhydryl reduction and proteolysis. The processed sperm nuclei were stained for DNA with the fluorochrome, 4′-6-diamidino-2-phenylindole (DAPI) before quantification by flow cytometry. Scanning and transmission electron micrographs of sperm heads taken at various steps of the preparation and staining procedures show 1) that the rigorous washing procedure disrupted the plasma and outer acrosomal membranes, 2) that ethanol fixation resulted in removal of the outer membranes and disintegration of the nuclear envelope, and 3) that thiol and proteolysis treatment removed the remaining cellular organelles including the tail and rapidly induced partial decondensation of the tightly packed chromatin. Sequential micrographs showed that the nuclear matrix of all three species increased in thickness about twofold during the preparation and staining. Consequently, the harsh procedures currently used for quantitative staining of DNA for high-resolution flow cytometric analyses destroy most cellular organelles and thereby prevent simultaneous characterization of DNA content and other sperm cell constituents.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Publication Date: 2019-07-27
    Description: An experimental version of a thinned illuminated buried-channel 512 x 320 pixel CCD with reduced amplifier input capacitance has been produced which is characterized by lower readout noise. Changes made to the amplifier are discussed, and readout noise measurements obtained by several different techniques are presented. The single energetic electron response of the CCD in the electron-bombarded mode and the single 5.9 keV X-ray pulse height distribution are reported. Results are also given on the dark current versus temperature and the spatial frequency response as a function of signal level.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Solid state imagers for astronomy; June 10, 11, 1981; Cambridge, MA
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  • 5
    Publication Date: 2011-08-19
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: NASA, Washington Upper Atmosphere Res. Program; p 234-235
    Format: text
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  • 6
    Publication Date: 2011-08-18
    Description: A diode-laser-based, ultrahigh resolution IR heterodyne spectrometer for laboratory and field use has been developed for operation between 7.5 and 8.5 microns. The local oscillator is a PbSe tunable diode laser kept continuously at operating temperatures of 12-60 K using a closed-cycle cooler. The laser output frequency is controlled and stabilized using a high-precision diode current supply, constant temperature controller, and a shock isolator mounted between the refrigerator cold tip and the diode mount. The system largely employs reflecting optics to minimize losses from internal reflection and absorption and to eliminate chromatic effects. Spectral analysis of the diode-laser output between 0 and 1 GHz reveals excess noise at many diode current settings, which limits the IR spectral regions over which useful heterodyne operation can be achieved. Observations have been made of atmospheric N2O, O3, and CH4 between 1170 and 1200/cm, using both a single-frequency swept IF channel and a 64-channel RF spectral line receiver with a total IF coverage of 1600 MHz.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Applied Optics; 21; Jan. 15
    Format: text
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  • 7
    Publication Date: 2011-08-19
    Description: Attention is given to 1.2 years of data from two Michelson-Gale long fluid tiltmeters, one long center pressure tiltmeter, and a shallow borehole tiltmeter, which have been in operation at Pinon Flat Observatory in Southern California. The most stable tilt record shows a secular rate of 0.28 microrad/a, which may be real. Comparisons undertaken among instruments have identified more and less successful measurement techniques. It is concluded that even the best existing sensors must be improved in order to measure continuous tectonic motions.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Geophysical Research Letters (ISSN 0094-8276); 11; 963-966
    Format: text
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  • 8
    Publication Date: 2016-06-07
    Description: A redesign of the antenna electronics package for the NBS/GPS C/A receiver has resulted in significantly reduced cost and improved performance. Major improvements include a simplified and more reliable multiplier/mixer, elimination of all twelve piston trimmer tuning capacitors in the original design, elimination of expensive bandpass filters, less expensive antenna and a simplified packaging scheme.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: NASA. Goddard Space Flight Center Proc. of the 16th Ann. Precise Time and Time Interval (PTTI) Appl. and Planning Meeting; p 467-474
    Format: application/pdf
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  • 9
    Publication Date: 2019-07-27
    Description: A soft X-ray/XUV imaging camera which uses a thinned, back-illuminated, all-buried channel RCA CCD for radiation sensing has been built and tested. The camera is a slow-scan device which makes possible frame integration if necessary. The detection characteristics of the device have been tested over the 15-1500 eV range. The response was linear with exposure up to 0.2-0.4 erg/sq cm; saturation occurred at greater exposures. Attention is given to attempts to resolve single photons with energies of 1.5 keV.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Solid state imagers for astronomy; June 10, 11, 1981; Cambridge, MA
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  • 10
    Publication Date: 2019-07-27
    Description: The employment of area focal plane arrays (FPA) has made it possible to obtain second generation infrared imaging systems with high resolution and sensitivity. The Short Wavelength Infrared (SWIR) region (1-2.5 microns) is of importance for imaging objects at high temperature and under conditions of reflected sunlight. The present investigation is concerned with electrooptical characterization results for 32 x 32 SWIR detector arrays and FPAs which are suitable for use in a prototype imaging spectrometer. The employed detector material is Hg(1-x)Cd(x)Te grown by liquid phase epitaxy on a CdTe transparent substrate. Attention is given to details of processing, the design of the detector array, the multiplexer, the fabrication of the hybrid FPA, and aspects of performance.
    Keywords: INSTRUMENTATION AND PHOTOGRAPHY
    Type: Technical issues in infrared detectors and arrays; April 5, 6, 1983; Arlington, VA
    Format: text
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