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  • 1
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    Transgene and transposon silencing in Chlamydomonas reinhardtii by a DEAH-box RNA helicase (2000)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2000-11-10
    Description: The molecular mechanism(s) responsible for posttranscriptional gene silencing and RNA interference remain poorly understood. We have cloned a gene (Mut6) from the unicellular green alga Chlamydomonas reinhardtii that is required for the silencing of a transgene and two transposon families. Mut6 encodes a protein that is highly homologous to RNA helicases of the DEAH-box family. This protein is necessary for the degradation of certain aberrant RNAs, such as improperly processed transcripts, which are often produced by transposons and some transgenes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wu-Scharf, D -- Jeong, B -- Zhang, C -- Cerutti, H -- New York, N.Y. -- Science. 2000 Nov 10;290(5494):1159-62.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉School of Biological Sciences and Plant Science Initiative, University of Nebraska-Lincoln, E211 Beadle Center, Post Office Box 880666, Lincoln, NE 68588, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/11073454" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Motifs ; Amino Acid Sequence ; Animals ; Chlamydomonas reinhardtii/enzymology/*genetics ; Cloning, Molecular ; *DNA Transposable Elements ; *Gene Silencing ; Humans ; Molecular Sequence Data ; RNA/metabolism ; RNA Helicases/chemistry/*genetics/*metabolism ; RNA, Messenger/metabolism ; Retroelements ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Alignment ; Sequence Homology, Amino Acid ; *Transgenes
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase (1988)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1988-01-29
    Description: A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus, greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10(5) cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Saiki, R K -- Gelfand, D H -- Stoffel, S -- Scharf, S J -- Higuchi, R -- Horn, G T -- Mullis, K B -- Erlich, H A -- New York, N.Y. -- Science. 1988 Jan 29;239(4839):487-91.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2448875" target="_blank"〉PubMed〈/a〉
    Keywords: Cloning, Molecular ; DNA/*genetics ; DNA, Recombinant ; DNA-Directed DNA Polymerase/*metabolism ; Electrophoresis, Agar Gel ; Globins/genetics ; *Hot Temperature ; Humans ; *Nucleic Acid Amplification Techniques ; Nucleic Acid Denaturation ; Nucleic Acid Hybridization ; RNA/genetics ; Thermus/enzymology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Antibodies in HIV-1 vaccine development and therapy (2013)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2013-09-14
    Description: Despite 30 years of study, there is no HIV-1 vaccine and, until recently, there was little hope for a protective immunization. Renewed optimism in this area of research comes in part from the results of a recent vaccine trial and the use of single-cell antibody-cloning techniques that uncovered naturally arising, broad and potent HIV-1-neutralizing antibodies (bNAbs). These antibodies can protect against infection and suppress established HIV-1 infection in animal models. The finding that these antibodies develop in a fraction of infected individuals supports the idea that new approaches to vaccination might be developed by adapting the natural immune strategies or by structure-based immunogen design. Moreover, the success of passive immunotherapy in small-animal models suggests that bNAbs may become a valuable addition to the armamentarium of drugs that work against HIV-1.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970325/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3970325/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Klein, Florian -- Mouquet, Hugo -- Dosenovic, Pia -- Scheid, Johannes F -- Scharf, Louise -- Nussenzweig, Michel C -- AI 100148-01/AI/NIAID NIH HHS/ -- AI 100663-01/AI/NIAID NIH HHS/ -- P01 AI081677/AI/NIAID NIH HHS/ -- P01 AI100148/AI/NIAID NIH HHS/ -- UM1AI100663/AI/NIAID NIH HHS/ -- Howard Hughes Medical Institute/ -- New York, N.Y. -- Science. 2013 Sep 13;341(6151):1199-204. doi: 10.1126/science.1241144.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA. fklein@rockefeller.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/24031012" target="_blank"〉PubMed〈/a〉
    Keywords: AIDS Vaccines/*therapeutic use ; Acquired Immunodeficiency Syndrome/prevention & control/*therapy ; Antibodies, Neutralizing/biosynthesis/genetics/*immunology ; HIV Antibodies/biosynthesis/genetics/*immunology ; HIV Infections/*therapy ; HIV-1/*immunology ; Humans ; Immunotherapy ; Viral Envelope Proteins/immunology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Rebound insomnia: a new clinical syndrome (1978)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1978-09-15
    Description: Rebound insomnia followed the withdrawal of three benzodiazepine hypnotic drugs, each of which had been administered in a single nightly dose for only short-term periods. The intense worsening of sleep is attributed to the short duration of the action of these drugs. A hypothesis involving benzodiazepine receptors in the brain is proposed in which there is a delay or lag in replacement of endogenous benzodiazepine-like molecules after the abrupt withdrawal of exogenous drugs.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kales, A -- Scharf, M B -- Kales, J D -- New York, N.Y. -- Science. 1978 Sep 15;201(4360):1039-41.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/684426" target="_blank"〉PubMed〈/a〉
    Keywords: Benzodiazepines/*adverse effects/metabolism ; Brain/metabolism ; Flunitrazepam/adverse effects/metabolism ; Humans ; Hypnotics and Sedatives/*adverse effects/metabolism ; Nitrazepam/adverse effects/metabolism ; Receptors, Drug/drug effects/metabolism ; Sleep Initiation and Maintenance Disorders/*etiology/metabolism ; *Substance Withdrawal Syndrome/metabolism ; Syndrome ; Time Factors ; Triazolam/adverse effects/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 5
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    Cigarette smoking associated with sleep difficulty (1980)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1980-02-01
    Description: A group of 50 smokers experienced greater sleep difficulty than a group of 50 nonsmokers matched by age and sex. The two groups did not differ in personality patterns or drug consumption. Also, sleep patterns significantly improved in a group of eight chronic smokers when they abstained from cigarette smoking. These findings are consistent with reports on the stimulant effects of nicotine.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Soldatos, C R -- Kales, J D -- Scharf, M B -- Bixler, E O -- Kales, A -- New York, N.Y. -- Science. 1980 Feb 1;207(4430):551-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/7352268" target="_blank"〉PubMed〈/a〉
    Keywords: Adult ; Coffee/adverse effects ; Female ; Humans ; Male ; Sleep Stages ; Sleep Wake Disorders/*etiology ; Smoking/*complications ; Substance Withdrawal Syndrome/physiopathology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia (1985)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1985-12-20
    Description: Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies. In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end-labeled oligonucleotide probe hybridized in solution to the amplified beta-globin sequences. The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Saiki, R K -- Scharf, S -- Faloona, F -- Mullis, K B -- Horn, G T -- Erlich, H A -- Arnheim, N -- New York, N.Y. -- Science. 1985 Dec 20;230(4732):1350-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2999980" target="_blank"〉PubMed〈/a〉
    Keywords: Alleles ; Anemia, Sickle Cell/*diagnosis/genetics ; Base Sequence ; Clinical Laboratory Techniques ; DNA Restriction Enzymes ; DNA-Directed DNA Polymerase ; Escherichia coli ; *Gene Amplification ; Globins/*genetics ; Humans ; Nucleic Acid Hybridization ; Polymorphism, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    Direct cloning and sequence analysis of enzymatically amplified genomic sequences (1986)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1986-09-05
    Description: A method is described for directly cloning enzymatically amplified segments of genomic DNA into an M13 vector for sequence analysis. A 110-base pair fragment of the human beta-globin gene and a 242-base pair fragment of the human leukocyte antigen DQ alpha locus were amplified by the polymerase chain reaction method, a procedure based on repeated cycles of denaturation, primer annealing, and extension by DNA polymerase I. Oligonucleotide primers with restriction endonuclease sites added to their 5' ends were used to facilitate the cloning of the amplified DNA. The analysis of cloned products allowed the quantitative evaluation of the amplification method's specificity and fidelity. Given the low frequency of sequence errors observed, this approach promises to be a rapid method for obtaining reliable genomic sequences from nanogram amounts of DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Scharf, S J -- Horn, G T -- Erlich, H A -- New York, N.Y. -- Science. 1986 Sep 5;233(4768):1076-8.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/3461561" target="_blank"〉PubMed〈/a〉
    Keywords: Base Sequence ; Cloning, Molecular/*methods ; Coliphages/*genetics ; DNA Polymerase I/metabolism ; Gene Amplification ; *Genetic Vectors ; Globins/*genetics ; HLA-DQ Antigens ; Histocompatibility Antigens Class II/*genetics ; Humans ; In Vitro Techniques ; Polymorphism, Genetic
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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