Publication Date:
2010-10-12
Description:
For proper partitioning of chromosomes in mitosis, the chromosomal passenger complex (CPC) including Aurora B and survivin must be localized at the center of paired kinetochores, at the site called the inner centromere. It is largely unknown what defines the inner centromere and how the CPC is targeted to this site. Here, we show that the phosphorylation of histone H3-threonine 3 (H3-pT3) mediated by Haspin cooperates with Bub1-mediated histone 2A-serine 121 (H2A-S121) phosphorylation in targeting the CPC to the inner centromere in fission yeast and human cells. H3-pT3 promotes nucleosome binding of survivin, whereas phosphorylated H2A-S121 facilitates the binding of shugoshin, the centromeric CPC adaptor. Haspin colocalizes with cohesin by associating with Pds5, whereas Bub1 localizes at kinetochores. Thus, the inner centromere is defined by intersection of two histone kinases.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yamagishi, Yuya -- Honda, Takashi -- Tanno, Yuji -- Watanabe, Yoshinori -- New York, N.Y. -- Science. 2010 Oct 8;330(6001):239-43. doi: 10.1126/science.1194498.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20929775" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Aurora Kinase B
;
Aurora Kinases
;
Cell Cycle Proteins/metabolism
;
Centromere/*metabolism
;
Chromatin/metabolism
;
Chromosomal Proteins, Non-Histone/metabolism
;
Chromosome Segregation
;
Chromosomes, Fungal/*physiology
;
Chromosomes, Human/*physiology
;
HeLa Cells
;
Heterochromatin/metabolism
;
Histones/*metabolism
;
Humans
;
Inhibitor of Apoptosis Proteins
;
Intracellular Signaling Peptides and Proteins/chemistry/genetics/*metabolism
;
Kinetochores/metabolism
;
Microtubule-Associated Proteins/metabolism
;
Mitosis
;
Molecular Sequence Data
;
Mutation
;
Nucleosomes/metabolism
;
Phosphorylation
;
Protein Binding
;
Protein Interaction Domains and Motifs
;
Protein-Serine-Threonine Kinases/chemistry/genetics/*metabolism
;
Schizosaccharomyces/*genetics/metabolism
;
Schizosaccharomyces pombe Proteins/genetics/*metabolism
;
Serine/metabolism
;
Threonine/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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