Publication Date:
2009-12-08
Description:
Bub1 is a multi-task protein kinase required for proper chromosome segregation in eukaryotes. Impairment of Bub1 in humans may lead to chromosomal instability (CIN) or tumorigenesis. Yet, the primary cellular substrate of Bub1 has remained elusive. Here, we show that Bub1 phosphorylates the conserved serine 121 of histone H2A in fission yeast Schizosaccharomyces pombe. The h2a-SA mutant, in which all cellular H2A-S121 is replaced by alanine, phenocopies the bub1 kinase-dead mutant (bub1-KD) in losing the centromeric localization of shugoshin proteins. Artificial tethering of shugoshin to centromeres largely restores the h2a-SA or bub1-KD-related CIN defects, a function that is evolutionally conserved. Thus, Bub1 kinase creates a mark for shugoshin localization and the correct partitioning of chromosomes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kawashima, Shigehiro A -- Yamagishi, Yuya -- Honda, Takashi -- Ishiguro, Kei-ichiro -- Watanabe, Yoshinori -- New York, N.Y. -- Science. 2010 Jan 8;327(5962):172-7. doi: 10.1126/science.1180189. Epub 2009 Nov 19.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratory of Chromosome Dynamics, Institute of Molecular and Cellular Biosciences, University of Tokyo, Yayoi, Tokyo 113-0032, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19965387" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Centromere/*metabolism
;
*Chromosomal Instability
;
Chromosomal Proteins, Non-Histone/genetics/*metabolism
;
*Chromosome Segregation
;
Chromosomes, Fungal/metabolism
;
Histones/*metabolism
;
Humans
;
Kinetochores/metabolism
;
Meiosis
;
Mice
;
Mitosis
;
Nucleosomes/metabolism
;
Phosphorylation
;
Protein-Serine-Threonine Kinases/genetics/*metabolism
;
Recombinant Proteins/metabolism
;
Saccharomyces cerevisiae/genetics/metabolism
;
Saccharomyces cerevisiae Proteins/genetics/metabolism
;
Schizosaccharomyces/cytology/genetics/*metabolism
;
Schizosaccharomyces pombe Proteins/genetics/*metabolism
;
Serine/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
